Comparative genomic hybridization analysis detected frequent overrepresentation of chromosome 3q in squamous cell carcinoma of the lung

Chujo, Masao; Noguchi, Tsuyoshi; Miura, Takashi; Arinaga, Mitsuyuki; Uchida, Yuzo; Tagawa, Yutaka

doi:10.1016/s0169-5002(02)00151-4

Cited by 64 publications

(47 citation statements)

References 30 publications

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“…Amplification of 7p11.2 is frequently found in many cancer types, including lung cancer, breast cancer, and glioblastomas (27)(28)(29). By overlapping the amplified regions among different samples, we further verified that the minimal amplicon contained only EGFR and SEC61G.…”

Section: Discussionmentioning

confidence: 56%

Functional Genomic Analysis Identified Epidermal Growth Factor Receptor Activation as the Most Common Genetic Event in Oral Squamous Cell Carcinoma

et al. 2009

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A 250K single-nucleotide polymorphism array was used to study subchromosomal alterations in oral squamous cell carcinoma (OSCC). The most frequent amplification was found at 7p11.2 in 9 of 29 (31%) oral cancer patients. Minimal genomic mapping verified a unique amplicon spanning from 54.6 to 55.3 Mb on chromosome 7, which contains SEC61G and epidermal growth factor receptor (EGFR). Results from fluorescence in situ hybridization, transcriptome, and immunohistochemistry analyses indicated that the expression level of EGFR , but not of SEC61G, was up-regulated and tightly correlated with DNA copy number in 7p11.2 amplified tumors. Among the members of the erbB family, EGFR (HER1) was found to be the most frequently amplified and highly expressed gene in both human and mouse oral tumors (P < 0.01). Genes for downstream effectors of EGFR, including KRAS, mitogen-activated protein kinase 1, and CCND1, were also found amplified or mutated, which resulted in activation of EGFR signaling in 55% of OSCC patients. Head and neck squamous cancer cells with different EGFR expression levels showed differential sensitivity to antitumor effects of AG1478, a potent EGFR inhibitor. AG1478-induced EGFR inactivation significantly suppressed tumor development and progression in a mouse oral cancer model. Our data suggest that EGFR signaling is important in oral cancer development and that anti-EGFR therapy would benefit patients who carry the 7p11.2 amplicon in their tumors.

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Section: Discussionmentioning

confidence: 56%

Functional Genomic Analysis Identified Epidermal Growth Factor Receptor Activation as the Most Common Genetic Event in Oral Squamous Cell Carcinoma

et al. 2009

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“…Copy number increases of the 3q26-qter region have been identified in different SCCs, including lung, oesophagus and cervix. [27][28][29][30] The presence of frequent copy number gains/amplifications suggested the presence of a relevant proto-oncogene in this region.…”

Section: Discussionmentioning

confidence: 99%

High sex determining region Y-box 2 expression is a negative predictor of occult lymph node metastasis in early squamous cell carcinomas of the oral cavity

Züllig

Roessle

Weber

et al. 2013

European Journal of Cancer

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“…The gain of 3q has been reported in numerous tumour types, most frequently in those of squamous origin, including HNSCC, squamous lung carcinomas and cervical carcinoma. (Chujo et al, 2002;Struski, 2002). In squamous cell carcinoma of the uterine cervix, the gain of 3q has been shown to define the transition from severe dysplasia to invasive carcinoma (Heselmeyer et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of genomic alterations in head and neck squamous cell carcinoma detected by comparative genomic hybridisation

et al. 2003

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A total of 45 primary head and neck squamous cell carcinomas were analysed by comparative genomic hybridisation to identify regions of chromosomal deletion and gain. Multiple regions of copy number aberration were identified including gains affecting chromosomes 3q, 8q, 5p, 7q, 12p and 11q and deletion of material from chromosomes 3p, 11q, 4p, 5q, 8p, 10q, 13q and 21. KaplanMeier survival analysis revealed significant correlations between gain of 3q25 -27 and deletion of 22q with reduced disease-specific survival. In addition, gain of 17q and 20q, deletion of 19p and 22q and amplification of 11q13 were significantly associated with reduced disease-free survival. A Cox proportional hazards regression model identified deletion of 22q as an independent prognostic marker. The data presented here provide further evidence that the creation of a genetically based tumour classification system will soon be possible, complementing current histopathological characterisation.

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Comparative genomic hybridization analysis detected frequent overrepresentation of chromosome 3q in squamous cell carcinoma of the lung

Cited by 64 publications

References 30 publications

Functional Genomic Analysis Identified Epidermal Growth Factor Receptor Activation as the Most Common Genetic Event in Oral Squamous Cell Carcinoma

Functional Genomic Analysis Identified Epidermal Growth Factor Receptor Activation as the Most Common Genetic Event in Oral Squamous Cell Carcinoma

High sex determining region Y-box 2 expression is a negative predictor of occult lymph node metastasis in early squamous cell carcinomas of the oral cavity

Prognostic value of genomic alterations in head and neck squamous cell carcinoma detected by comparative genomic hybridisation

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