Comparative genomics of vancomycin-resistant Enterococcus spp. revealed common resistome determinants from hospital wastewater to aquatic environments

Ekwanzala, Mutshiene Deogratias; Dewar, John Barr; Kamika, Ilunga; Momba, Maggy Ndombo Benteke

doi:10.1016/j.scitotenv.2020.137275

Cited by 31 publications

(24 citation statements)

References 79 publications

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Section: Persistence Of Vre Strains In Portuguese Food Animals Anmentioning

confidence: 99%

“…VRE are found in wastewater treatment plants and in pig breeding facilities. The environmental prevalence of VRE is troublesome, as the release of effluents into watercourses and the use of sludge in agriculture might actively contribute to the dissemination of VRE strains, resistant bacteria and resistance genes throughout the environment [64]. Furthermore, the persistence of VRE in food-producing animals and related environments (years after avoparcin withdrawal) indicates that coselection with other antimicrobial agents increased fitness of strains and the presence of specific mobile genetic elements cannot be ruled out.…”

Section: Persistence Of Vre Strains In Portuguese Food Animals An Examplementioning

confidence: 99%

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Enterococci, from Harmless Bacteria to a Pathogen

et al. 2020

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Enterococci are gastrointestinal commensals whose hardiness allowed them to colonize very diverse environments, including soils, water, food, and feed. This ability to overcome adverse conditions makes enterococci problematic once they colonize hospital niches. Together with the malleability of their genomes, the capacity to acquire and disseminate determinants of antibiotic resistance has contributed to converting what was once just another opportunistic pathogen into a first-class clinical problem. This review discusses the dimension of the emergence of enterococcal resistance to key antimicrobial agents, the dissemination of this resistance, and its significance in terms of public health, with the aim of raising awareness of the need to devise and implement surveillance programs and more effective antibiotic stewardship.

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Section: Persistence Of Vre Strains In Portuguese Food Animals Anmentioning

confidence: 99%

Section: Persistence Of Vre Strains In Portuguese Food Animals An Examplementioning

confidence: 99%

Enterococci, from Harmless Bacteria to a Pathogen

et al. 2020

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“…Thus, its assessment should shed light on the transfer of antibiotics from WWTP to agricultural soils. On the other hand, when it comes to RS, recent reports have highlighted a high occurrence of resistant bacteria and genes [ 26 , 27 ]. Knowing that riverbed sediment can resuspend and re-enter the water column [ 34 ], it is important to assess such a vital component of the RW system.…”

Section: Discussionmentioning

confidence: 99%

“…Samples collected as part of a 24-h composite sample were collected on arrival at the municipal wastewater plant from the IW, AS, and EW compartments. For the sampling of HW and RW, a method previously used by Ekwanzala et al [ 26 , 27 ] was followed, where one sample consisted of a composite of five grab samples collected at 30-min intervals for better environmental representativeness of the matrix sampled. RW samples were collected within 100 m of the WWTP outlet (EW).…”

Section: Methodsmentioning

confidence: 99%

Environmental Dissemination of Selected Antibiotics from Hospital Wastewater to the Aquatic Environment

et al. 2020

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The environmental dissemination of selected antibiotics from hospital wastewater into municipal wastewater and lastly to a receiving water body was investigated. Selected antibiotics (azithromycin (AZM), ciprofloxacin (CIP), clindamycin (CDM), doxycycline (DXC) and sulfamethoxazole (SMZ)) present in effluents of academic hospital wastewater, influents, sewage sludge, and effluents of municipal wastewater, receiving water, and its benthic sediment samples were quantified using the Acquity® Waters Ultra-Performance Liquid Chromatography System hyphenated with a Waters Synapt G2 coupled to a quadrupole time-of-flight mass spectrometer. The overall results showed that all assessed antibiotics were found in all matrices. For solid matrices, river sediment samples had elevated concentrations with mean concentrations of 34,834, 35,623, 50,913, 55,263, and 41,781 ng/g for AZM, CIP, CDM, DXC, and SMZ, respectively, whereas for liquid samples, hospital wastewater and influent of wastewater had the highest concentrations. The lowest concentrations were observed in river water, with mean concentrations of 11, 97, 15, and 123 ng/L, except for CDM, which was 18 ng/L in the effluent of wastewater. The results showed that the highest percentages of antibiotics removed was SMZ with 90%, followed by DXC, AZM and CIP with a removal efficiency of 85%, 83%, and 83%, respectively. The antibiotic that showed the lowest removal percentage was CDM with 66%. However, the calculated environmental dissemination analysis through the use of mass load calculations revealed daily release of 15,486, 14,934, 1526, 922, and 680 mg/d for SMZ, CIP, AZM, DXC, and CDM, respectively, indicating a substantial release of selected antibiotics from wastewater to the river system, where they are possibly adsorbed in the river sediment. Further research into the efficient removal of antibiotics from wastewater and the identification of antibiotic sources in river sediment is needed.

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“…VRE are found in wastewater treatment plants and in pig breeding facilities. The environmental prevalence of VRE is troublesome, as the release of effluents into watercourses and the use of sludge in agriculture might actively contribute to the dissemination of VRE strains, resistant bacteria and resistance genes throughout the environment [64]. Furthermore, the persistence of VRE in food-producing animals and related environments (years after avoparcin withdrawal) indicates that co-selection with other antimicrobial agents increased fitness of strains and the presence of specific mobile genetic elements cannot be ruled out.…”

Section: Persistence Of Vre Strains In Portuguese Food Animals An Examplementioning

confidence: 99%

Enterococci, from Harmless Bacteria to a Pathogen

Ramos

Silva

Dapkevicius

et al. 2020

Preprint

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Enterococci are gastrointestinal commensals whose hardiness allowed them to colonize very diverse environments, including soils, water, food and feed. This ability to overcome adverse conditions makes enterococci problematic once they colonize hospital niches. Together with the malleability of their genomes, the capacity to acquire and disseminate determinants of antibiotic resistance have contributed to convert what was once just another opportunistic pathogen into a first-class clinical problem. This review discusses the dimension of the emergence of enterococcal resistance to key antimicrobial agents, the dissemination of this resistance and its significance in terms of public health, with the aim of raising the awareness to the need to devise and implement monitoring programmes and effective antibiotic usage guidelines.

show abstract

Comparative genomics of vancomycin-resistant Enterococcus spp. revealed common resistome determinants from hospital wastewater to aquatic environments

Cited by 31 publications

References 79 publications

Enterococci, from Harmless Bacteria to a Pathogen

Enterococci, from Harmless Bacteria to a Pathogen

Environmental Dissemination of Selected Antibiotics from Hospital Wastewater to the Aquatic Environment

Enterococci, from Harmless Bacteria to a Pathogen

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