Comparative high‐throughput analysis of sperm membrane proteins from crossbred bulls with contrasting fertility

Saraf, Kaustubh Kishor; Kumaresan, A.; Arathi, Bangalore Prabhashankar; Sundaresan, Nagalingam R.; Datta, Tirtha Kumar

doi:10.1111/and.14451

Cited by 3 publications

(4 citation statements)

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“…D’Amours et al found the most of the DAPs in HF bull sperm were related to energy metabolism, specifically, the glycolytic pathway member TPI1; adenylate kinase-8, which is involved in ATP production; the transaminase OAT, which enables ATP production through the entry of its product (glutamate) into the tricarboxylic citric acid cycle; and the protein DBIL5, which has been shown to have structural and binding similarity to acetyl CoA binding protein (ACBP) . In addition, Saraf et al found that OXPHOS and the citrate cycle were the most significantly affected pathways in low-fertility crossbred bull sperm …”

Section: Discussionmentioning

confidence: 99%

“…22 In addition, Saraf et al found that OXPHOS and the citrate cycle were the most significantly affected pathways in low-fertility crossbred bull sperm. 27 The hallmarks in LF bull spermatozoa are isoforms of the chaperone HSP90 (HSP90AA1 and HSP90B1), members of the chaperonin containing the TCP1 complex, and components of the 20S core proteasome complex (PSMA4 and PSMB4). These results could probably indicate defects in protein folding and proteostasis for this group.…”

Section: Discussionmentioning

confidence: 99%

“…New advances in technology have improved proteomics techniques in the last years, and mass spectrometry (MS) has become the method of choice due to its unrivaled ability to analyze complex protein mixtures like spermatozoa. Data-dependent acquisition (DDA) is a widely used mode of data collection in tandem with mass spectrometry (MS/MS) sperm proteins from Holstein Friesian − or crossbreed bulls. − In 2013, Gillet et al proposed a new proteomic strategy using a data-independent acquisition (DIA) method called SWATH-MS (sequential window acquisition of all theoretical mass spectra). The SWATH-MS method is particularly innovative among the DIA methods and presents several advantages (reproducibility and consistency, data acquisition, and quantifiable proteins) compared with DDA-based methods and targeted proteomics. , Sperm proteomic analysis using SWATH-MS provides highly valuable information on cellular components or sperm function, which, together with the data obtained from the in vitro parameters obtained by FC or CASA, offers a more complete picture of biological events.…”

Section: Introductionmentioning

confidence: 99%

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Bull Sperm SWATH-MS-Based Proteomics Reveals Link between High Fertility and Energy Production, Motility Structures, and Sperm–Oocyte Interaction

Gacem,

Castello-Ruiz,

Hidalgo

et al. 2023

J. Proteome Res.

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The prediction of male or semen fertility potential remains a persistent challenge that has yet to be fully resolved. This work analyzed several in vitro parameters and proteome of spermatozoa in bulls cataloged as high-(HF; n = 5) and low-field (LF; n = 5) fertility after more than a thousand artificial inseminations. Sperm motility was evaluated by computer-assisted sperm analysis. Sperm viability, mitochondrial membrane potential (MMP) and reactive oxygen species (mROS) of spermatozoa were assessed by flow cytometry. Proteome was evaluated by the SWATH-MS procedure. Spermatozoa of HF bulls showed significantly higher total motility than the LF group (41.4% vs 29.7%). Rates of healthy sperm (live, high MMP, and low mROS) for HF and LF bull groups were 49% and 43%, respectively (p > 0.05). Spermatozoa of HF bulls showed a higher presence of differentially abundant proteins (DAPs) related to both energy production (COX7C), mainly the OXPHOS pathway, and the development of structures linked with the motility process (TPPP2, SSMEM1, and SPAG16). Furthermore, we observed that equatorin (EQTN), together with other DAPs related to the interaction with the oocyte, was overrepresented in HF bull spermatozoa. The biological processes related to protein processing, catabolism, and protein folding were found to be overrepresented in LF bull sperm in which the HSP90AA1 chaperone was identified as the most DAP. Data are available via ProteomeXchange with identifier PXD042286.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bull Sperm SWATH-MS-Based Proteomics Reveals Link between High Fertility and Energy Production, Motility Structures, and Sperm–Oocyte Interaction

Gacem,

Castello-Ruiz,

Hidalgo

et al. 2023

J. Proteome Res.

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“…Therefore, proteomic studies would be beneficial to decipher the roles of sperm proteins on fertility. During the last decade, the proteomic approach helped to find out answers for many of hitherto unanswered questions related to sperm function and bull fertility [17][18][19][20] . Very recently, a preliminary study conducted using 2D-DIGE approach reported that sperm proteins associated with energy metabolism and capacitation were differentially expressed between high-and low-fertile buffalo bulls 21 .…”

mentioning

confidence: 99%

Establishment of a repertoire of fertility associated sperm proteins and their differential abundance in buffalo bulls (Bubalus bubalis) with contrasting fertility

Kumaresan

Sinha²,

Paul³

et al. 2023

Sci Rep

Self Cite

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Sperm harbours a wide range of proteins regulating their functions and fertility. In the present study, we made an effort to characterize and quantify the proteome of buffalo bull spermatozoa, and to identify fertility associated sperm proteins through comparative proteomics. Using high-throughput mass spectrometry platform, we identified 1305 proteins from buffalo spermatozoa and found that these proteins were mostly enriched in glycolytic process, mitochondrial respiratory chain, tricarboxylic acid cycle, protein folding, spermatogenesis, sperm motility and sperm binding to zona pellucida (p < 7.74E−08) besides metabolic (p = 4.42E−31) and reactive oxygen species (p = 1.81E−30) pathways. Differential proteomic analysis revealed that 844 proteins were commonly expressed in spermatozoa from both the groups while 77 and 52 proteins were exclusively expressed in high- and low-fertile bulls, respectively. In low-fertile bulls, 75 proteins were significantly (p < 0.05) upregulated and 176 proteins were significantly (p < 0.05) downregulated; these proteins were highly enriched in mitochondrial respiratory chain complex I assembly (p = 2.63E−07) and flagellated sperm motility (p = 7.02E−05) processes besides oxidative phosphorylation pathway (p = 6.61E−15). The down regulated proteins in low-fertile bulls were involved in sperm motility, metabolism, sperm-egg recognition and fertilization. These variations in the sperm proteome could be used as potential markers for the selection of buffalo bulls for fertility.

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Bisphenol A Negatively Impacts Human Sperm MicroRNA and Protein Profiles

Santiago,

Simková,

Silva

et al. 2024

Expo Health

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Bisphenol A (BPA), a widely prevalent endocrine disruptor, has detrimental effects on human health and male reproduction. Elevated BPA levels have been linked to reduced sperm quality and production, while paternal exposure may result in poor reproductive outcomes and offspring health. For many years, the effects of BPA exposure in human reproduction were mainly attributed to the deregulation of the hypothalamic–pituitary–gonadal (HPG) axis. In the past decades, other mechanisms of action have been proposed, and studies highlighting epigenetic alterations linked to BPA in sperm emerged. Nevertheless, the impact of BPA on human sperm miRNA and protein profiles and its implications for fertilization and post-fertilization events remain unexplored. This study aimed to investigate the association between BPA concentration in seminal plasma and seminal quality and to examine the alterations in human sperm miRNA and protein expression among men with varying BPA levels in seminal plasma. BPA levels were measured in seminal plasma from 102 Portuguese men, and it was detected in 88% of the samples. No correlation was observed between BPA levels and age or seminal parameters. The small RNA content of 15 normozoospermic samples was evaluated using small RNA sequencing, revealing that 15 miRNAs exhibited a correlation with BPA levels. Gene ontology analysis of their target genes indicated their involvement in embryonic development and response to stress. The proteome of 20 normozoospermic human sperm samples, categorized into four groups based on BPA levels, was accessed by liquid chromatography–mass spectrometry. Data are available via ProteomeXchange with the identifier PXD047280. Sixty-two differentially expressed proteins were identified across groups. The biological processes most affected in samples with higher BPA levels were “protein sumoylation” and “cytoplasmic translation”. The altered miRNAs and proteins identified in spermatozoa from men environmentally exposed to BPA represent potential biological markers of exposure to this endocrine disruptor and may help elucidate cases of idiopathic male infertility, fertilization failure, and abnormal embryo development.

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Comparative high‐throughput analysis of sperm membrane proteins from crossbred bulls with contrasting fertility

Cited by 3 publications

References 69 publications

Bull Sperm SWATH-MS-Based Proteomics Reveals Link between High Fertility and Energy Production, Motility Structures, and Sperm–Oocyte Interaction

Bull Sperm SWATH-MS-Based Proteomics Reveals Link between High Fertility and Energy Production, Motility Structures, and Sperm–Oocyte Interaction

Establishment of a repertoire of fertility associated sperm proteins and their differential abundance in buffalo bulls (Bubalus bubalis) with contrasting fertility

Bisphenol A Negatively Impacts Human Sperm MicroRNA and Protein Profiles

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