Comparative histological study on wound healing on rat's skin treated with Mitomycin C or Clobetasol propionate

Carvalho, Maria de Fátima Pereira; Pereira, Celina Siqueira Barbosa; Fregnani, José Humberto Tavares Guerreiro; Ribeiro, Fernando de Andrade Quintanilha

doi:10.1590/s0102-865020150090000002

Cited by 10 publications

(6 citation statements)

References 15 publications

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“…Hair of dorsum of all experimental rats was shaved at the same time using a standard hair shaver. Clobetasol propionate was applied in form of 0.05% cream at a dosage of 0.25g/kg once a day for 15 successive days [10] .…”

Section: • Induction Of Skin Atrophymentioning

confidence: 99%

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Effect of Curcumin versus Hyaluronic Acid on Glucocorticoid Induced Skin Atrophy and Subsequent Skin Abrasions in Rats

Fawzy

Ahmed

Elshafie

et al. 2019

Egyptian Journal of Histology

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Background: Skin is the largest organ of the body in surface area and weight. One of the most important side effects of topical glucocorticoids therapy is skin atrophy. This study aimed to evaluate the possible therapeutic effects of curcumin versus aftamed gel (Hyaluronic Acid) on glucocorticoid-induced skin atrophy, as well as on subsequently induced abrasion wounds. Methods: Forty two adult male albino rats were divided into 4 groups: Group1 (Control group) of 6 rats, Group II (Steroid-Exposed Group), Group III (Curcumin-Treated Group) and Group IV (Aftamed-treated group). Each of group II, III and IV were subdivided into two subgroups IIA, IIIA, IVA (subjected to clobetasol propionate cream) each included 6 rats and IIB, IIIB, IVB (subjected to clobetasol propionate cream, then exposed to skin abrasion) each included 6 rats. Skin biopsies were taken from the dorsum of rats 12th days from the stoppage of clobetasol propionate application. Sections were stained with hematoxylin and eosin, Masson's trichrome and immunohistochemichal stain for PCNA & CK19. Epidermal thicknesses, mean area percent of collagen fibers and mean number of PCNA & CK19 immunopositive cells were measured. Results: Steroid-exposed group showed thinning of epidermal and dermal layers with scanty hair follicles and sebaceous glands as well as delay in wound healing. Increased epidermal thickness and reorganized dermal structural elements with increased PCNA & CK19 immunopositive cells were more obvious in curcumin treated group than aftamed treated group. Conclusion: topical application of curcumin had more improving effect on skin and its abrasions rather than aftamed gel.

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Section: • Induction Of Skin Atrophymentioning

confidence: 99%

“…• Subgroup IIA: subjected to clobetasol propionate 0.05% cream topically in a dose of (0.25g/kg) once daily for 15 days [10] . Then this group were lifted untreated for 12 days, then were sacrificed.…”

Section: • Experimental Designmentioning

confidence: 99%

Effect of Curcumin versus Hyaluronic Acid on Glucocorticoid Induced Skin Atrophy and Subsequent Skin Abrasions in Rats

Fawzy

Ahmed

Elshafie

et al. 2019

Egyptian Journal of Histology

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“…These observations were also confirmed by histology, in which 5 days post-injury, a healthy healing process was evidenced by the formation of a crust and by the appearance of granulation tissue (marked by green arrows). This healthy process was verified when fresh epidermis tissue was created after 15 days. , After 12 days, both PVA groups showed complete re-epithelization and hair growth, compared with the untreated group, which required 15 days to heal.…”

Section: Resultsmentioning

confidence: 78%

“…This healthy process was verified when fresh epidermis tissue was created after 15 days. 38,39 After 12 days, both PVA groups showed complete re-epithelization and hair growth, compared with the untreated group, which required 15 days to heal. The incorporation of B. subtilis into PVA microparticles, which demonstrated excellent in vitro performance, was relatively limited in the case of the in vivo healing process.…”

Section: Surfactin and Fengycinmentioning

confidence: 97%

Bacillus subtilis in PVA Microparticles for Treating Open Wounds

et al. 2021

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Open wound dressings should provide a moist environment, protect the wound from bacterial contamination, and shield it from further damage. These requirements, however, are hard to accomplish since such wounds are colonized by pathogenic bacteria, including resistant species such as methicillin-resistant Staphylococcus aureus (MRSA). A new approach for treating open wounds that is based on sticky and dissolvable polyvinyl alcohol (PVA) microparticles containing live Bacillus subtilis (B. subtilis) is described. Microparticles, fabricated by the spray-drying technique, were administered directly to an open wound while B. subtilis continuously produced and secreted antimicrobial molecules. B. subtilis in PVA microparticles demonstrated remarkable antibacterial activity against MRSA and S. aureus. In in vivo experiments, both B. subtilis and empty PVA microparticles were effective in decreasing healing time; however, B. subtilis microparticles were more effective during the first week. There was no evidence of skin irritation, infection, or other adverse effects during the 15 day postoperative observation period. This concept of combining live secreting bacteria within a supportive delivery system shows great promise as a therapeutic agent for open wounds and other infectious skin disorders.

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“…After rats were anaesthetized and skin hairs on the back were removed, the skin was sterilized by using ethanol (70%), and next, a 20-mm diameter circle was drawn. A circular cut was made around the drawn surface area of skin; the skin was then carefully dissected to create a full thickness wound [ 54 ]. The wound of the excision region was recorded instantly.…”

Section: Methodsmentioning

confidence: 99%

A Gelatin Hydrogel-Containing Nano-Organic PEI–Ppy with a Photothermal Responsive Effect for Tissue Engineering Applications

et al. 2018

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The introduction and designing of functional thermoresponsive hydrogels have been recommended as recent potential therapeutic approaches for biomedical applications. The development of bioactive materials such as thermosensitive gelatin-incorporated nano-organic materials with a porous structure and photothermally triggerable and cell adhesion properties may potentially achieve this goal. This novel class of photothermal hydrogels can provide an advantage of hyperthermia together with a reversibly transformable hydrogel for tissue engineering. Polypyrrole (Ppy) is a bioorganic conducting polymeric substance and has long been used in biomedical applications owing to its brilliant stability, electrically conductive features, and excellent absorbance around the near-infrared (NIR) region. In this study, a cationic photothermal triggerable/guidable gelatin hydrogel containing a polyethylenimine (PEI)–Ppy nanocomplex with a porous microstructure was established, and its physicochemical characteristics were studied through dynamic light scattering, scanning electronic microscopy, transmission electron microscopy, an FTIR; and cellular interaction behaviors towards fibroblasts incubated with a test sample were examined via MTT assay and fluorescence microscopy. Photothermal performance was evaluated. Furthermore, the in vivo study was performed on male Wistar rat full thickness excisions model for checking the safety and efficacy of the designed gelatin–PEI–Ppy nanohydrogel system in wound healing and for other biomedical uses in future. This photothermally sensitive hydrogel system has an NIR-triggerable property that provides local hyperthermic temperature by PEI–Ppy nanoparticles for tissue engineering applications. Features of the designed hydrogel may fill other niches, such as being an antibacterial agent, generation of free radicals to further improve wound healing, and remodeling of the promising photothermal therapy for future tissue engineering applications.

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Comparative histological study on wound healing on rat's skin treated with Mitomycin C or Clobetasol propionate

Abstract: The actions of Mitomycin C and Clobetasol were equivalent and not interfere in fibroplasias and in angiogenesis. Both drugs initially cause a decrease in collagen over a 30-day period and an increase over a 60-day period, demonstrating a delay in the wound healing.

Cited by 10 publications

References 15 publications

Effect of Curcumin versus Hyaluronic Acid on Glucocorticoid Induced Skin Atrophy and Subsequent Skin Abrasions in Rats

Effect of Curcumin versus Hyaluronic Acid on Glucocorticoid Induced Skin Atrophy and Subsequent Skin Abrasions in Rats

Bacillus subtilis in PVA Microparticles for Treating Open Wounds

A Gelatin Hydrogel-Containing Nano-Organic PEI–Ppy with a Photothermal Responsive Effect for Tissue Engineering Applications

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