Comparative histopathology in mouse typhoid among genetically diverse mice

Abstract

Genetically resistant CBA and A/J mice and susceptible BALB/c and C57BL/6 mice were challenged with either an identical infective dose or a minimal lethal dose of Salmonella typhimurium. The histopathological progression of the disease was examined in tissue sections prepared by the JB-4 Plus resin embedding method and compared between the resistant and susceptible mice. In a fatal disease, the lesions in both animal hosts began with focal abscesses within the first three days post infection. Mononuclear cell infiltration started by day 4 and transformed the lesions into granulomata. Well-formed granulomata were evident by day 7 and persisted in sublethally infected resistant mice. Massive bacterial proliferation and extensive tissue degeneration marked the terminal stage of a lethal challenge. There were no distinguishable features that would identify the tissue response to infection in a resistant host from a susceptible one, except that the lesions in the sublethally infected resistant mice advanced slower and were discrete and self-limiting.

Terminal Deoxynucleotidyl Transferase Is Not Required for Antibody Response to Polysaccharide Vaccines against Streptococcus pneumoniae and Salmonella enterica Serovar Typhi

“…The liver lesions observed in S. Typhimurium infection in mice (33)(34)(35)(36)(37) are very similar to those described for human typhoid patients (38)(39)(40). We confirmed that systemic infection of naive mice with live S. Typhimurium strain RC60 resulted in extensive liver damage, as shown by large areas of hepatocyte necrosis and infiltration of mononuclear cells (Fig.…”