Comparative immunogenicity of outer membrane protein K and whole‐cell antigens of
            <i>Vibrio parahaemolyticus</i>
            for diagnosis

Wang, Wenbin; Guo, Songlin; Gao, Yuwei; Liang, Xianwei; Liu, Lei; Pan, Saikun

doi:10.1111/lam.13536

Cited by 6 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…But the synthesis of the OmpU protein is regulated by NaCl content of the cultured medium, OmpU accounts for 30% of the total outer membrane protein when V. cholerae cultured in 1.0% NaCl medium and 60% in without NaCl [ 39 ]. Besides, the outer membrane protein OmpK with nucleoside transmembrane transport protein activity is highly conserved in Vibrio , which has been recognized [ 41 ]. There have some proteins with efflux transmembrane transporter activity also conserved in various NaCl stress cultured, such as outer membrane channel protein ( tolC ), outer membrane protein ( VIBR0546_13282 ), and outer membrane protein ( VIBR0546_20323 ) that have not been reported.…”

Section: Resultsmentioning

confidence: 99%

Effects of NaCl Concentration on the Behavior of Vibrio brasiliensis and Transcriptome Analysis

Wang

et al. 2022

Foods

View full text Add to dashboard Cite

The growth of Vibrio bacteria is affected by environmental conditions, and unfavorable conditions will produce different degrees of stress on Vibrio. The cells respond to the stress on the bacteria through changes in biological characteristics and transcriptomes. To study the effect of NaCl concentration on Vibrio brasiliensis, we have determined the biological characteristics of the 0%, 1%, 2%, 3%, 5%, and 7% NaCl concentrations cultured V. brasiliensis to research the salt stress to bacteria. We found that the biological properties of V. brasiliensis cultured with different NaCl concentrations were different, and the expression of outer membrane proteins of V. brasiliensis changed when it was grown under different NaCl concentrations. When bacteria cultured in higher NaCl concentrations (3%, 5% and 7% NaCl), the sodium-type flagellar protein MotY was found. Finally, the transcriptome analysis of V. brasiliensis cultured with 0% NaCl and 7% NaCl was carried out to find out the differentially expressed genes. We found that the same gene have opposite up-regulated and down-regulated expression in two treatments, indicating that these types of genes are regulated different in low and high osmotic stress.

show abstract

Section: Resultsmentioning

confidence: 99%

Effects of NaCl Concentration on the Behavior of Vibrio brasiliensis and Transcriptome Analysis

Wang

et al. 2022

Foods

View full text Add to dashboard Cite

show abstract

“…For instance, in a study using recombinant OmpK, recombinant GAPDH, and a fusion of both antigens as possible vaccines, although antibody titration from vaccinated groups was significantly different from the control group, the protective effect upon challenge was better using the fusion of both antigens ( 88 ). In another study ( 89 ), serum from mice immunized with recombinant OmpK from V. parahaemolyticus only reacted with homologous V. parahaemolyticus , indicating that recombinant OmpK may not be the ideal target antigen for the development of a polyvalent vaccine.…”

Section: Discussionmentioning

confidence: 99%

Oral vaccination of fish against vibriosis using spore-display technology

et al. 2022

View full text Add to dashboard Cite

Oral vaccines are highly demanded by the aquaculture sector, to allow mass delivery of antigens without using the expensive and labor-intensive injectable vaccines. These later require individual handling of fish, provoking stress-related mortalities.One possible strategy to create injection-free vaccine delivery vehicles is the use of bacterial spores, extremely resistant structures with wide biotechnological applications, including as probiotics, display systems, or adjuvants. Bacterial spores, in particular those of Bacillus subtilis, have been shown to behave as mucosal vaccine adjuvants in mice models. However, such technology has not been extensively explored against fish bacterial disease.In this study, we used a laboratory strain of B. subtilis, for which a variety of genetic manipulation tools are available, to display at its spores surface either a Vibrio antigenic protein, OmpK, or the green fluorescence protein, GFP. When previously vaccinated by immersion with the OmpK- carrying spores, zebrafish survival upon a bacterial challenge with V. anguillarum and V. parahaemolyticus, increased up to 50 - 90% depending on the pathogen targeted. Further, we were able to detect anti-GFP-antibodies in the serum of European seabass juveniles fed diets containing the GFP-carrying spores and anti-V. anguillarum antibodies in the serum of European seabass juveniles fed the OmpK-carrying spores containing diet. More important, seabass survival was increased from 60 to 86% when previously orally vaccinated with in-feed OmpK- carrying spores. Our results indicate that B. subtilis spores can effectively be used as antigen-carriers for oral vaccine delivery in fish.

show abstract

“…A study on cross-protection also found a recombinant cell vaccine had successfully induced an immune response to V. parahaemolyticus in juvenile sea bass by expressing the OmpK of Vibrio [ 27 ]. At the same time, another study has pointed out the limitation of recombinant OmpK in preparing diagnostic antibodies [ 44 ]. For this reason, using modern methods for understanding and developing new V. parahaemolyticus immunogenic proteins and antibodies are necessary [ 45 , 46 ].…”

Section: Control and Prevention Strategies Of Vibriosmentioning

confidence: 99%

Strategies for Prevention and Control of Vibriosis in Asian Fish Culture

Wang

Yang

et al. 2022

Vaccines

View full text Add to dashboard Cite

It is estimated that vibriosis account for about half of the economic losses in Asian fish culture. Consequently, the prevention and control of vibriosis is one of the priority research topics in the field of Asian fish culture disease. Relevant measures have been proposed to control some Vibrios that pose a threat to Asian fish culture, but there are currently only a few effective vaccines available to combat these Vibrios. The purpose of our review is to sum up the main prevention methods and the latest control strategies of seven Vibrio species that cause great harm to Asian aquaculture, including Vibrio harveyi, Vibrio vulnificus, Vibrio parahaemolyticus, Vibrio mimicus, Vibrio anguillarum, Vibrio alginolyticus and Vibrio cholerae. Strategies such as antibiotics, probiotics, bacteriophages, antimicrobials from plants and other natural sources, as well as vaccines, are compared and discussed here. We expect this review will provide some new views and recommendations for the future better prevention and control of vibriosis in Asian fish culture.

show abstract

Comparative immunogenicity of outer membrane protein K and whole‐cell antigens of Vibrio parahaemolyticus for diagnosis

Cited by 6 publications

References 34 publications

Effects of NaCl Concentration on the Behavior of Vibrio brasiliensis and Transcriptome Analysis

Effects of NaCl Concentration on the Behavior of Vibrio brasiliensis and Transcriptome Analysis

Oral vaccination of fish against vibriosis using spore-display technology

Strategies for Prevention and Control of Vibriosis in Asian Fish Culture

Contact Info

Product

Resources

About