Comparative Immunological, Biological and Biophysical Behaviour of Meningopneumonitis Viral Antigenic Preparations

Parikh, G. C.; Shechmeister, I. L.

doi:10.1038/2041013a0

Cited by 5 publications

(1 citation statement)

References 9 publications

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“…embryo-allantoic fluids as well as in media of we reasoned that if the rapid destructive action infected cultured cells (7,8,30,31). Culture of C. psittaci upon mouse macrophages in the medium from 6BC infected L cells was therefore intact animal (1 5) and the toxic effect of C. used for the production of agent-free agglutinat-psittaci in mouse rnacrophages in vitro are ing material as described previously (8).…”

Section: Cj~totoxic Eflect In~lucerl By Heat-inactivatedmentioning

confidence: 99%

Lysosomes and the "toxicity" of Rickettsiales. I. Cytochemical studies of macrophages inoculated in vitro with C. psittaci 6BC

Kordová¹,

Wilt²

1972

Can. J. Microbiol.

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Toxic doses of C. psittaci 6BC strain produced within 3–6 h after inoculation a rapidly progressive irreversible damage of mouse macrophages in vitro, an early leakage of a lysosomal enzyme, acid phosphatase, as well as a rapid disintegration of neutral red-stained cytoplasmic granules i.e. lysosomes. Pretreatment of the agent with immune serum neutralized the cytotoxic action of the agent and a reversible activation of lysosomes occurred. Pretreatment of the agent with undiluted control serum protected the macrophages from the early "toxic" injury; however, a cytopathic effect (CPE) occurred 48 h after inoculation and was accompanied by a release of lysosomal acid phosphatase. Heat-inactivated Chlamydiae produced an early agglutination of macrophages with leakage of lysosomal acid phosphatase, disintegration of neutral red-stained lysosomes, as well as a progressive irreversible damage of the macrophage. Concentrated, heat-inactivated agent-free, leuco-agglutinating material from 6BC infected L cells produced a similar injury of macrophages and their lysosomes. The findings are related to an earlier report of a rapid destruction of macrophages by C. psittaci in the intact animal (15). The possible mechanisms and factors of the "toxicity" of Rickettsiales are discussed.

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Section: Cj~totoxic Eflect In~lucerl By Heat-inactivatedmentioning

confidence: 99%

Lysosomes and the "toxicity" of Rickettsiales. I. Cytochemical studies of macrophages inoculated in vitro with C. psittaci 6BC

Kordová¹,

Wilt²

1972

Can. J. Microbiol.

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Agglutination of white blood cells by meningopneumonitis organisms

Parikh

Shechmeister

1967

J. Pathol.

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Multiplication of meningopneumonitis organisms in white blood cells

Shechmeister

Parikh

1968

J. Pathol.

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REPORTS on phagocytosis of viruses and the organisms of the psittacosislymphogranuloma-trachoma group are sporadic (Sabin, 1935 ; Florman and Enders, 1942; Merling, 1945).Observations of the fate of intracellular virus are comparatively more recent (Barski and Robbe-Fossat, 1957; Benedict and McFarland, 1958). The interaction of viruses with leucocytes has been shown to lead to (1) alteration in certain of the metabolic and phagocytic activities of the leucocytes (Merchant and Morgan, 1950; Fisher and Ginsberg, 1956), (2) agglutination of the leucocytes (Nungester, Gordon and Collins, 1950; Boand, Kempf and Hanson, 1957; Nishmi and Fkrnkopf, 1958; Shechmeister and Parikh, 1961 ; Parikh and Shechmeister, 1967), and (3) phagocytosis and multiplication of the virus (Dunne, Luedke and Hokanson, 1958; Parikh, Shechmeister and Yen, 1963). Smorodmtsev (1960), in reviewing the role of leucocytes in viral infections, stated that phagocytosis is not an important natural defence mechanism, except with the psittacosis-lymphogranuloma-trachoma group. However, the role of blood leucocytes, macrophages and other cells of the reticulo-endothelial system in the viral diseases has not been studied systematically. This is particularly true for phagocytosis of viruses by leucocytes in vitro and in vivo, which may explain in a relatively simple manner certain aspects of virus-host cell interaction.This report presents evidence for phagocytosis and multiplication of meningopneumonitis organisms (MP) in leucocytes (WBC) and stresses the importance of the MP:WBC ratio in determining the extent of intraleucocytic replication. MATERIALS AND hETHOD.5 Preparation of MP organismsProduction and concentration of MP suspensions. The Cal 10 strain of MP organisms, originally isolated by Francis and Magill (1938) was kindly supplied by Dr J. M. Moulder of the University of Chicago. The organisms were grown in the yolk sac of embryonated hen eggs by inoculating 0.5 ml. of a 1 in 100 dilution of the agent in each 5-to 6-day-old chick embryo and harvesting the yolk sac after 5 days' incubation at 37°C. No antibiotics were used, and freedom from bacterial contamination was determined by culturing in thioglycollate broth. Preliminary estimates of the concentration of MP were made by examination of smears of yolksac membrane stained with Macchiavello's stain. Infected yolk sac was ground in a homogeniser, or by mortar and pestle, in nutrient broth, the ratio of yolk-sac material to broth being 1 in 10, and then centrifuged at 4°C at 3020g for 30 min. Yolk-sac material and debris were discarded, and the supernatant was recentrifuged

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Comparative Immunological, Biological and Biophysical Behaviour of Meningopneumonitis Viral Antigenic Preparations

Cited by 5 publications

References 9 publications

Lysosomes and the "toxicity" of Rickettsiales. I. Cytochemical studies of macrophages inoculated in vitro with C. psittaci 6BC

Lysosomes and the "toxicity" of Rickettsiales. I. Cytochemical studies of macrophages inoculated in vitro with C. psittaci 6BC

Agglutination of white blood cells by meningopneumonitis organisms

Multiplication of meningopneumonitis organisms in white blood cells

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