Comparative immunological recognition of proteins from New Guinea "C" dengue virus type 2 prototype and from a dengue virus type 2 strain isolated in the State of Rio de Janeiro, Brazil

Côrtes, Luzia Monteiro de Castro; Barth, Ortrud Monika; Pantoja, Josery R; Alves, Carlos Roberto

doi:10.1590/s0074-02762003000300007

Cited by 3 publications

(4 citation statements)

References 18 publications

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“…Several authors have reported a significant antibody response to NS3 in both primary and secondary infections (Churdboonchart et al 1991, Valdes et al 2000, Cortes et al 2003. In contrast, a low but significant and specific antibody response to NS3 in 79% of the confirmed dengue cases was observed in this study, and none of the sera from healthy individuals was able to recognize this protein.…”

contrasting

confidence: 87%

“…Later the same group of researchers (AbuBakar et al 2002) demonstrated, using infected A. albopictus (C6/36) mosquito cells as antigen, antibody responses to the antigenic intermediate core protein (C)-prM and to the nonstructural proteins NS1 and NS2A. Cortes et al (2003) evaluated antibody recognition profiles of DENV-2 (Rio de Janeiro isolated) and DENV-2 NGC proteins and specific recognition for proteins NS3, E, NS1, C, and prM was detected. It is clear that the antigen quality and origin will produce different results, and some antibody responses to some viral proteins may not be detected.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Antibody Response in Human Dengue Patients from the Mexican Coast Using Recombinant Antigens

Lázaro-Olan

Mellado-Sánchez²,

García‐Cordero

et al. 2008

Vector-Borne and Zoonotic Diseases

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This study was undertaken to evaluate the feasibility of using recombinant dengue proteins to discriminate between acute dengue infections versus uninfected dengue samples. Dengue virus proteins E, NS1, NS3, and NS4B were cloned as fusion proteins and expressed in Escherichia coli. Recombinant products were tested in 100 serum samples obtained from acute dengue fever cases collected from 3 states of Mexico where dengue is endemic. Sera from 75 healthy individuals living in nonendemic areas for dengue were used as a control group. In sera from the dengue patients group, antibody responses to E protein were demonstrated in 91% of cases and NS1 protein was recognized to various extents (99%) within the first 7 days of infection. The antibody responses to NS3 and NS4B were frequently of low magnitude. Consistent negative antibody responses to all proteins were found in sera from the control group. These data suggest that the glutathione-S-transferase (GST)-dengue fusion proteins may be feasible antigens for a sensitive and specific serological assay.

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confidence: 87%

Section: Introductionmentioning

confidence: 99%

Analysis of Antibody Response in Human Dengue Patients from the Mexican Coast Using Recombinant Antigens

Lázaro-Olan

Mellado-Sánchez²,

García‐Cordero

et al. 2008

Vector-Borne and Zoonotic Diseases

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“…A good correlation between the results of immunoblot strips and type-specific RT-PCR was observed in primary infections (see below) [54]. Other immunoblot kits for dengue diagnosis have been available for several years [3,47,55,56], despite being usually unable to detect antibodies to the NS1 protein in primary infections. A recent report demonstrated the detection of dengue NS1 antigen in serum or plasma in the absence of anti-dengue IgM antibodies, with some advantages over RT-PCR (see section on PCR-based techniques), namely the possibility of storing the samples at 4 C for retrospective testing and the absence of contamination due to the higher stability of antigens [57].…”

Section: Dot-blot Immunoassaymentioning

confidence: 85%

Trends in dengue diagnosis

2005

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The conventional diagnosis of dengue virus infections includes the detection of the virus in serum or tissue samples, both by isolation in culture or through detection of specific viral molecules (genome RNA or dengue antigens) and detection of specific anti-dengue antibodies (serology). Isolation of dengue virus provides the most direct and conclusive approach to diagnosis, despite the demand for high-level equipment, technical skills and manpower. However, it is useless in early diagnosis because several days are required to isolate and classify the virus. Serology, despite being simpler, is not able to afford an accurate early diagnosis in primary infections because 4-5 days are required for the immune system to produce a sufficient amount of antibodies. Moreover, it leads to misleading results in secondary infections owing to cross-reactivity among serotype-specific antibodies and with other flavivirus antibodies. The RT-PCR and other PCR-based techniques are fast, serotype-discriminating, more sensitive and easier to carry out than conventional nucleic-acid hybridisation, but are handicapped by easy sample contamination and high technological demands. Recently, advances in bioelectronics have generated commercial kits and new techniques for detection of dengue antibodies and RNA, based on biosensor technology. Most of them are rapid, easy to operate, reusable, cheap, sensitive and serotype-specific. Nevertheless, their accuracy is still questionable because most still lack validation and standardisation. This review summarises and describes the techniques currently employed and anticipated in the near future for diagnosis of dengue disease.

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“…NS3 protein plays a predominant role in the pathogenesis of the disease and together with its role during the processing of the precursor of the viral polyprotein makes it an interesting protein for evaluating host responses. Some authors have reported a significant antibody response to NS3 in both primary and secondary infections [39–41]. However, Valdes et al, 2000 [40], showed a significant antibody response in secondary cases when the infecting serotype was DEN2.…”

Section: Discussionmentioning

confidence: 99%

Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus

Álvarez-Rodríguez

Ramos-Ligonio

Rosales‐Encina

et al. 2012

Journal of Tropical Medicine

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Dengue is one of the major public health concerns in the world. Since all the four serotypes are actively circulating in Mexico, there is a need to develop an efficient diagnosis system to improve case management of the patients. There exist few studies evaluating the use of the NS3 protein as a protective antigen against dengue virus (DENV). In this paper we show the expression of a recombinant NS3 protein from all serotypes of dengue virus (GST-DVNS3-1-4) and report a reliable “in-house detection system” for the diagnosis of dengue infection which was field-tested in a small village (Tezonapa) in the state of Veracruz, Mexico. The fusion proteins were immunogenic, inducing antibodies to be able to recognize to antigens up to a 1 : 3200 dilution. The purified proteins were used to develop an in-house detection system (ELISA) and were further tested with a panel of 239 serum samples. The in-house results were in excellent agreement with the commercial kits with κ = 0.934 ± 0.064 (95% CI = 0.808–1.061), and κ = 0.872 ± 0.048 (95% CI = 0.779–0.965) for IgM and IgG, respectively. The agreement between the NS1 antigen detection versus the rNS3 ELISA, κ = 0.837 ± 0.066 (95% CI = 0.708–0.966), was very good. Thus, these results demonstrate that recombinant NS3 proteins have potential in early diagnosis of dengue infections.

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Comparative immunological recognition of proteins from New Guinea "C" dengue virus type 2 prototype and from a dengue virus type 2 strain isolated in the State of Rio de Janeiro, Brazil

Abstract: The protein profiles of the New Guinea "C" dengue virus type 2

Cited by 3 publications

References 18 publications

Analysis of Antibody Response in Human Dengue Patients from the Mexican Coast Using Recombinant Antigens

Analysis of Antibody Response in Human Dengue Patients from the Mexican Coast Using Recombinant Antigens

Trends in dengue diagnosis

Expression, Purification, and Evaluation of Diagnostic Potential and Immunogenicity of a Recombinant NS3 Protein from All Serotypes of Dengue Virus

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