Comparative in vitro Activity of 8 Cephalosporins on 109 Strains of &lt;i&gt;Neisseria gonorrhoeae&lt;/i&gt; and 60 Strains of &lt;i&gt;Neisseria meningitidi&lt;/i&gt;&lt;i&gt;s&lt;/i&gt;

Thabaut, A; Durosoir, J. L.; Saliou, Philippe

doi:10.1159/000238024

Cited by 13 publications

(8 citation statements)

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“…Preliminary studies suggest that a single dose of between 50 and 125 mg of ceftriaxone per kg is adequate for the treatment of uncomplicated gonorrhea (4). In vitro susceptibility tests have shown that the minimal inhibitory concentration of ceftriaxone for P-lactamase-producing N. gonorrhoeae is approximately 0.01 ,ug/ml (10). These observations and the results of the current study suggest that ceftriaxone would be effective as a single-dose regimen against mixed infections with T. pallidum and ,1-lactamase-positive N. gonorrhoeae.…”

Section: Discussionsupporting

confidence: 67%

Comparison of the activities of ceftriaxone and penicillin G against experimentally induced syphilis in rabbits

Johnson¹,

Bey²,

Wolgamot³

1982

Antimicrob Agents Chemother

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The activity of ceftriaxone, a newly developed cephalosporin, against early cutaneous infections with Treponema pallidum in rabbits was compared with that of equimolar doses of penicillin G. Activity was related to the time required for cutaneous lesions to become dark-field negative, serological response, and the disappearance of T. pallidum from the popliteal lymph nodes. Both antibiotics were very effective in the treatment of syphilis in this animal model. The 50% curative dose for penicillin G was 0.8 ,umol/kg (0.29 mg or 480 U/kg) and for ceftriaxone, it was 1.45 xmol/kg (0.96 mg/kg). Overall, ceftriaxone was slightly less effective than penicillin G was. Transmission and scanning electron microscopy studies of testicular aspirates obtained from rabbits treated with ceftriaxone revealed alterations in the treponeme surface which apparently resulted in changes in cell permeability and morphology.Ceftriaxone is a newly developed parenteral cephalosporin. It has a broader spectrum of activity, higher potency, and better r-lactamase stability than the older semisynthetic cephalosporins; it is also cleared from the body more

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Section: Discussionsupporting

confidence: 67%

Comparison of the activities of ceftriaxone and penicillin G against experimentally induced syphilis in rabbits

Johnson¹,

Bey²,

Wolgamot³

1982

Antimicrob Agents Chemother

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“…'9 20 Ceftriaxone is a new third generation cephalosporin that has been found to be active against both PPNG and non-PPNG strains.5 6 In the study published here the non-PPNG strains were more susceptible than PPNG strains to ceftriaxone, a finding noted previously by Thabaut et …”

Section: Discussionsupporting

confidence: 59%

Randomised comparative study of ceftriaxone and spectinomycin in gonorrhoea.

Panikabutra¹,

Ariyarit²,

Chitwarakorn³

et al. 1985

Sexually Transmitted Infections

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“…Cephalothin, a first-generation cephalosporin that is 325-fold less effective than ceftriaxone against N. gonorrhoeae (89), permitted the growth of the Δngo1205 mutant, resulting in a final mutant OD 600 of 0.101, while the WT did not grow at all (File S1). However, the presence of moxalactam, a third-generation cephalosporin that is over 20-fold more effective than cephalothin (89), allowed the WT to grow to an OD 600 of 0.195, while the mutant achieved an OD 600 of 0.13 (File S1).…”

Section: Figmentioning

confidence: 99%

Deciphering the Function of New Gonococcal Vaccine Antigens Using Phenotypic Microarrays

et al. 2017

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The function and extracellular location of cell envelope proteins make them attractive candidates for developing vaccines against bacterial diseases, including challenging drug-resistant pathogens, such as Neisseria gonorrhoeae. A proteomicsdriven reverse vaccinology approach has delivered multiple gonorrhea vaccine candidates; however, the biological functions of many of them remain to be elucidated. Herein, the functions of six gonorrhea vaccine candidates-NGO2121, NGO1985, NGO2054, NGO2111, NGO1205, and NGO1344 -in cell envelope homeostasis were probed using phenotype microarrays under 1,056 conditions and a ΔbamE mutant (Δngo1780) as a reference of perturbed outer membrane integrity. Optimal growth conditions for an N. gonorrhoeae phenotype microarray assay in defined liquid medium were developed, which can be useful in other applications, including rapid and thorough antimicrobial susceptibility assessment. Our studies revealed 91 conditions having uniquely positive or negative effects on one of the examined mutants. A cluster analysis of 37 and 57 commonly beneficial and detrimental compounds, respectively, revealed three separate phenotype groups: NGO2121 and NGO1985; NGO1344 and BamE; and the trio of NGO1205, NGO2111, and NGO2054, with the last protein forming an independent branch of this cluster. Similar phenotypes were associated with loss of these vaccine candidates in the highly antibiotic-resistant WHO X strain. Based on their extensive sensitivity phenomes, NGO1985 and NGO2121 appear to be the most promising vaccine candidates. This study establishes the principle that phenotype microarrays can be successfully applied to a fastidious bacterial organism, such as N. gonorrhoeae. IMPORTANCE Innovative approaches are required to develop vaccines against prevalent and neglected sexually transmitted infections, such as gonorrhea. Herein, we have utilized phenotype microarrays in the first such investigation into Neisseria gonorrhoeae to probe the function of proteome-derived vaccine candidates in cell envelope homeostasis. Information gained from this screening can feed the vaccine candidate decision tree by providing insights into the roles these proteins play in membrane permeability, integrity, and overall N. gonorrhoeae physiology. The optimized screening protocol can be applied in investigations into the function of other hypothetical proteins of N. gonorrhoeae discovered in the expanding number of whole-genome sequences, in addition to revealing phenotypic differences between clinical and laboratory strains.KEYWORDS Neisseria gonorrhoeae, vaccine antigens, cell envelope, phenotypic microarrays, antibiotics, Biolog I n Gram-negative bacteria, the cell envelope is composed of the outer membrane, the cell wall, and the cytoplasmic or inner membrane, each of which can be further broken down into its constituents (1). The outer membrane is a bilayer composed of a lipopolysaccharide (LPS) or lipooligosaccharide (LOS) outer leaflet facing the extracel-

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Comparative in vitro Activity of 8 Cephalosporins on 109 Strains of <i>Neisseria gonorrhoeae</i> and 60 Strains of <i>Neisseria meningitidi</i><i>s</i>

Cited by 13 publications

References 0 publications

Comparison of the activities of ceftriaxone and penicillin G against experimentally induced syphilis in rabbits

Comparison of the activities of ceftriaxone and penicillin G against experimentally induced syphilis in rabbits

Randomised comparative study of ceftriaxone and spectinomycin in gonorrhoea.

Deciphering the Function of New Gonococcal Vaccine Antigens Using Phenotypic Microarrays

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