Comparative in vitro cytotoxicity study of silver nanoparticle on two mammalian cell lines

Mukherjee, Sanchali Gupta; O’Claonadh, Niall; Casey, Alan; Chambers, Gary

doi:10.1016/j.tiv.2011.12.004

Cited by 173 publications

(124 citation statements)

References 45 publications

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“…A previous report showed that cell lines exhibiting low GSH levels were more sensitive to AgNPs than those with a greater GSH content. 48 In agreement with that study, we observe that selective depletion of GSH by BSO results in increased AgNP toxicity. Interestingly, modulation of GSH levels in triplenegative MDA-MB-231 cells has only a small effect on the cytotoxicity of AgNPs.…”

supporting

confidence: 92%

Differential cytotoxic and radiosensitizing effects of silver nanoparticles on triple-negative breast cancer and non-triple-negative breast cells

Swanner¹,

Mims²,

Carroll³

et al. 2015

IJN

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Identification of differential sensitivity of cancer cells as compared to normal cells has the potential to reveal a therapeutic window for the use of silver nanoparticles (AgNPs) as a therapeutic agent for cancer therapy. Exposure to AgNPs is known to cause dose-dependent toxicities, including induction of oxidative stress and DNA damage, which can lead to cell death. Triple-negative breast cancer (TNBC) subtypes are more vulnerable to agents that cause oxidative stress and DNA damage than are other breast cancer subtypes. We hypothesized that TNBC may be susceptible to AgNP cytotoxicity, a potential vulnerability that could be exploited for the development of new therapeutic agents. We show that AgNPs are highly cytotoxic toward TNBC cells at doses that have little effect on nontumorigenic breast cells or cells derived from liver, kidney, and monocyte lineages. AgNPs induced more DNA and oxidative damage in TNBC cells than in other breast cells. In vitro and in vivo studies showed that AgNPs reduce TNBC growth and improve radiation therapy. These studies show that unmodified AgNPs act as a self-therapeutic agent with a combination of selective cytotoxicity and radiation dose-enhancement effects in TNBC at doses that are nontoxic to noncancerous breast and other cells.

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supporting

confidence: 92%

Differential cytotoxic and radiosensitizing effects of silver nanoparticles on triple-negative breast cancer and non-triple-negative breast cells

Swanner¹,

Mims²,

Carroll³

et al. 2015

IJN

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“…Reduction of this enzyme could increase ROS leading to impairment of mitochondria. Studies have also shown that depletion of glutathione level and increased ROS were found in association with mitochondrial perturbation, suggesting that oxidative stress might mediate the cytotoxicity of silver nanoparticles (Chen and Schluesener, 2008;Kim et al, 2009;Mukherjee et al, 2012). These studies are in agreement with our findings.…”

Section: Discussionsupporting

confidence: 93%

In vitro effect of nanosilver on gene expression of superoxide dismutases and nitric oxide synthases in chicken sertoli cells

Hassanpour

Mirshokraei

Sadrabad

et al. 2015

Animal

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To evaluate effects of different concentrations of nanosilver colloid on the cell culture of Sertoli cells, the proportion of lipid peroxidation, antioxidant capacity, nitric oxide (NO) production and genes expression of superoxide dismutases (SOD1 and SOD2) and nitric oxide synthases (eNOS and iNOS) were measured. Sertoli cells were incubated at concentrations of 25, 75 and 125 ppm nanosilver for 48 h. There was progressive lipid peroxidation in treatments according to increasing of nanosilver. Lipid peroxidation, as indicated by malondialdehyde levels, was significantly elevated by the highest concentration of silver colloid (125 ppm), although antioxidant capacity, as measured by ferric ion reduction, was unaffected. Nitrite, as an index of NO production was reduced only in 125 ppm of nanosilver. Expression of SOD1 gene was reduced in nanosilver-treated cells at all concentrations, whereas expression of SOD2 gene was reduced only in cells treated with 125 ppm nanosilver. Expression of iNOS gene was progressively increased with higher concentrations of nanosilver. Expression of eNOS gene was also increased in 125 ppm of nanosilver. In conclusion, toxic effects of nanosilver could be due to high lipid peroxidation and suppression of antioxidant mechanisms via reduced expression of SOD genes and increased expression of NOS genes.

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“…In vitro assays demonstrated toxic effects on macrophages, lung epithelial cells, stem cells, liver cells, neuronal cells, osteoblasts and many others (Arora et al 2008, Asare et al 2012, AshaRani et al 2009, Hussain et al 2005, Moaddab et al 2011, Mukherjee et al 2012. As regards the mechanisms of toxic effects of silver nanoparticles, the infl uence on the formation of free radicals is primarily mentioned.…”

Section: Introductionmentioning

confidence: 99%

Effects of silver nanoparticles of different sizes on cytotoxicity and oxygen metabolism disorders in both reproductive and respiratory system cells

Zapór

2016

Archives of Environmental Protection

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Silver nanoparticles (AgNPs) are widely used in numerous industries and areas of daily life, mainly as antimicrobial agents. The particles size is very important, but still not suffi ciently recognized parameter infl uencing the toxicity of nanosilver. The aim of this study was to investigate the cytotoxic effects of AgNPs with different particle size ( 10, 40 and 100 nm). The study was conducted on both reproductive and pulmonary cells (CHO-9, 15P-1 and RAW264.7). We tested the effects of AgNPs on cell viability, cell membrane integrity, mitochondrial metabolic activity, lipid peroxidation, total oxidative and antioxidative status of cells and oxidative DNA damage. All kinds of AgNPs showed strong cytotoxic activity at low concentrations (2÷13 μg/ml), and caused an overproduction of reactive oxygen species (ROS) at concentrations lower than cytotoxic ones. The ROS being formed in the cells induced oxidative damage of DNA in alkaline comet assay. The most toxic was AgNPs<10 nm. The results indicate that the silver nanoparticles, especially less than 10 nm, may be harmful to the organisms. Therefore, risk should be considered when using nanosilver preparations and provide appropriate protective measures when they are applied.

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Comparative in vitro cytotoxicity study of silver nanoparticle on two mammalian cell lines

Cited by 173 publications

References 45 publications

Differential cytotoxic and radiosensitizing effects of silver nanoparticles on triple-negative breast cancer and non-triple-negative breast cells

Differential cytotoxic and radiosensitizing effects of silver nanoparticles on triple-negative breast cancer and non-triple-negative breast cells

In vitro effect of nanosilver on gene expression of superoxide dismutases and nitric oxide synthases in chicken sertoli cells

Effects of silver nanoparticles of different sizes on cytotoxicity and oxygen metabolism disorders in both reproductive and respiratory system cells

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