Comparative map between chicken Chromosome 15 and human chromosomal region 12q24 and 22q11-q12

Abstract

The physical and comparative map of GGA15 was improved by the construction of 9 BAC contigs around loci previously mapped on GGA15 by linkage analysis. In total, 240 BAC clones were isolated, covering 30-35% of GGA15, and 120 STS were developed (104 STS derived from BAC end sequences and 18 STS derived within genes). Seventeen chicken orthologues of human genes located on human Chr 22q11-q12 were directly mapped within BAC contigs of GGA15. Furthermore, the partial sequences of the chicken BAC clones were compared with sequences present in the EMBL/GenBank databases and revealed matches to 26 genes, ESTs, and genomic clones located on HSA22q11-q12 and HSA12q24. These results provide a better alignment of GGA15 with the corresponding regions in human and mouse, and improve our knowledge of the evolution and dynamics of the vertebrate genome.

The ZW Micro-Sex Chromosomes of the Chinese Soft-Shelled Turtle (<i>Pelodiscus sinensis</i>, Trionychidae, Testudines) Have the Same Origin as Chicken Chromosome 15

“…Avian and reptilian genomes are generally characterized by an extremely high level of karyotype conservation and/or conserved linkage homology of chromosomal segments within and between these taxa Guttenbach et al, 2003;de Oliveira et al, 2005;Matsuda et al, 2005;Matsubara et al, 2006;Nishida-Umehara et al, 2007;Kawai et al, 2007Kawai et al, , 2009Nishida et al, 2008]. This genetic linkage of the P. sinensis Z chromosome shared with GGA15 showed conserved synteny with human chromosomes 12 and 22 [Jennen et al, 2003;International Chicken Genome Sequencing Consortium, 2004;Bourque et al, 2005;Schmid et al, 2005]. This study is the first report showing conserved linkage homology of the turtle sex chromosome with avian and mammalian autosomes.…”

Second report on chicken genes and chromosomes 2005

“…Moreover, although human/chicken comparative mapping has been well studied so far Ladjali-Mohammedi et al, 2001;Buitenhuis et al, 2002;Jennen et al, 2002Jennen et al, , 2003Nanda et al, 2002a;, RH-mapping data will increase the resolution of the gene maps within the conserved syntenies. It will thus help in refining the synteny breakpoints as well as detecting internal rearrangements, as it has been done in other species (Barbazuk et al, 2000;Goureau et al, 2001;Smith S.F.…”

“…Four of these BAC libraries (Ren et al, 2003;Pieter de Jong, unpublished results) are derived from the one inbred Red Jungle fowl bird used in whole genome sequencing and the fifth library was derived from a White Leghorn bird (Crooijmans et al, 2000), a breed phenotype selected for egg production. Using chromosome walking, partial BAC contig maps have been constructed for a number of different chromosomes Buitenhuis et al, 2002;Jennen et al, 2002Jennen et al, , 2003 but these maps only covered between 10-50 % of these chromosomes. The construction of a physical BAC map of a complete vertebrate genome the size of that of the chicken is not feasible using chromosome walking.…”

Libraries of Large-Insert Genomic Clones as a Tool for Molecular Cytogenetic Analysis of Avian Genome

“…For instance, orthology of chromosome GGA15 regions and the regions of HAS12q24 and HSA22q11-q12 was established [39]. In addition, an association between GGA5 and HSA19 [40], as well as between GGA10 and HSA15 [41] was demonstrated.…”