Comparative microbiome analysis reveals bacterial communities associated with Candidatus Liberibacter asiaticus infection in the Huanglongbing insect vector Diaphorina citri

Liu, Kai; Pang, Rui; Guan, Ziying; Zhong, Mingzhao; He, Jiawei; Han, Qunxin

doi:10.1016/j.aspen.2022.101884

Cited by 6 publications

(10 citation statements)

References 47 publications

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“…Wolbachia plays critical roles on host immunity ( Teixeira, Ferreira & Ashburner, 2008 ; Osborne et al, 2012 ), immunocompetence ( Braquart-Varnier et al, 2008 ), fecundity ( Weeks et al, 2007 ), and metabolic activity ( Darby et al, 2014 ). Notably simple bacterial communities like these have been reported for other psyllids species, including the different haplotypes of B. cockerelli ( Morrow et al, 2020 ; Štarhová Serbina et al, 2022 ; Cooper et al, 2022 ; Liu et al, 2022 ; Nakabichi, Inoue & Hirose, 2022a , 2022b ; Jiang et al, 2023 ).…”

Section: Discussionsupporting

confidence: 64%

“…These findings are in accordance with previous reports where populations of Asian citrus psyllid ( Diaphorina citri ) are analyzed infected and non-infected with ‘ Ca . Liberibacter asiaticus ’ infection significantly increased in higher alpha diversity metrics than in non-infected individuals ( Liu et al, 2022 ; Jiang et al, 2023 ). Similar results were observed in insects of the Cicadellidae and Cixiidae family ( Euscelis incisus , Euscelidius variegatus , and Hyalesthes obsoletus ) infected with ‘Candidatus Phytoplasma solani’ ( Moussa et al, 2020 ).…”

Section: Discussionmentioning

confidence: 98%

“…The presence of plant pathogenic bacteria in the vector insects affects the abundance of some bacterial genera ( Fagen et al, 2012 ; Hosseinzadeh et al, 2019 ; Moussa et al, 2020 ; Liu et al, 2022 ; Jiang et al, 2023 ). In the present work, we determined that the samples with more presence of the ‘ Ca .…”

Section: Discussionmentioning

confidence: 99%

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Bacterial communities of the psyllid pest Bactericera cockerelli (Hemiptera: Triozidae) Central haplotype of tomato crops cultivated at different locations of Mexico

Caamal-Chan,

Barraza,

Loera-Muro

et al. 2023

PeerJ

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Background The psyllid, Bactericera cockerelli, is an insect vector of ‘Candidatus Liberibacter’ causing “Zebra chip” disease that affects potato and other Solanaceae crops worldwide. In the present study, we analyzed the bacterial communities associated with the insect vector Bactericera cockerelli central haplotype of tomato crop fields in four regions from Mexico. Methods PCR was used to amplify the mitochondrial cytochrome oxidase I gene (mtCOI) and then analyze the single nucleotide polymorphisms (SNP) and phylogenetic analysis for haplotype identification of the isolated B. cockerelli. Moreover, we carried out the microbial diversity analysis of several B. cockerelli collected from four regions of Mexico through the NGS sequencing of 16S rRNA V3 region. Finally, Wolbachia was detected by the wsp gene PCR amplification, which is the B. cockerelli facultative symbiont. Also we were able to confirm the relationship with several Wolbachia strains by phylogenetic analysis. Results Our results pointed that B. cockerelli collected in the four locations from Mexico (Central Mexico: Queretaro, and Northern Mexico: Sinaloa, Coahuila, and Nuevo Leon) were identified, such as the central haplotype. Analyses of the parameters of the composition, relative abundance, and diversity (Shannon index: 1.328 ± 0.472; Simpson index 0.582 ± 0.167), showing a notably relatively few microbial species in B. cockerelli. Analyses identified various facultative symbionts, particularly the Wolbachia (Rickettsiales: Anaplasmataceae) with a relative abundance higher. In contrast, the genera of Sodalis and ‘Candidatus Carsonella’ (Gammaproteobacteria: Oceanospirillales: Halomonadaceae) were identified with a relatively low abundance. On the other hand, the relative abundance for the genus ‘Candidatus Liberibacter’ was higher only for some of the locations analyzed. PCR amplification of a fragment of the gene encoding a surface protein (wsp) of Wolbachia and phylogenetic analysis corroborated the presence of this bacterium in the central haplotype. Beta-diversity analysis revealed that the presence of the genus ‘Candidatus Liberibacter’ influences the microbiota structure of this psyllid species. Conclusions Our data support that the members with the highest representation in microbial community of B. cockerelli central haplotype, comprise their obligate symbiont, Carsonella, and facultative symbionts. We also found evidence that among the factors analyzed, the presence of the plant pathogen affects the structure and composition of the bacterial community associated with B. cockerelli.

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Section: Discussionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 98%

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Bacterial communities of the psyllid pest Bactericera cockerelli (Hemiptera: Triozidae) Central haplotype of tomato crops cultivated at different locations of Mexico

Caamal-Chan,

Barraza,

Loera-Muro

et al. 2023

PeerJ

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“…As two dominant genera in the D. citri gut, it is unclear why Profftella and Wolbachia changed significantly with host plants. In addition to host plants, temperature [ 46 ], gender [ 46 ], and C Las-infected or not [ 47 ] could significantly affect the change of these two symbionts. Pantoea , Stenotrophomonas , Sphingomonas , Methylobacterium , Microbacterium , and Lactobacillus were classified as environmental or plant-associated microbes, which can be found during D. citri landing/evaluation/feeding steps on the host plants.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Gut Bacterial Communities of Asian Citrus Psyllid (Diaphorina citri) Reared on Different Host Plants

Meng

ChangXiu

Zhou

et al. 2022

Insects

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Diaphorina citri Kuwayama (Hemiptera: Liviidae) can cause severe damage to citrus plants, as it transmits Candidatus Liberibacter spp., a causative agent of Huanglongbing disease. Symbiotic bacteria play vital roles in the ecology and biology of herbivore hosts, thereby affecting host growth and adaptation. In our research, the effects of Rutaceous plants (i.e., Citrus reticulata cv. Shatangju, Citrus poonensis cv. Ponkan, Murraya paniculata (orange jasmine), Citrus limon (lemon), and Citrus sinensis (navel orange)) on the gut microbiota (GM) and microbial diversity of D. citri adults were investigated by 16S rRNA high-throughput sequencing. It was found that Proteobacteria dominated the GM communities. The gut microbe diversity was the highest in the ponkan-feeding population, and the lowest in the Shatangju-feeding population. The NMDS analysis revealed that there were obvious differences in the GM communities among the different hosts. PICRUSt function prediction indicated significant differences in host function, and those pathways were crucial for maintaining population reproduction, growth, development, and adaptation to environmental stress in D. citri. Our study sheds new light on the interactions between symbionts, herbivores, and host plants and expands our knowledge on host adaptation related to GM in D. citri.

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“…The gut microbes are indispensable because they offer benefits to the host in multiple aspects, including detoxification of plant allelochemicals, provision of nutrition for host insects, and digestion of complex carbohydrates [ 15 , 16 ]. Intestinal microorganisms are engaged in several life processes of host insects, such as maintaining intestinal homeostasis, increasing oviposition, fecundity, and longevity of the host, controlling the insect immune system to protect against injury from external organisms, and altering insect behavior [ 17 – 19 ]. The microorganisms of D. citri have also recently received widespread attention.…”

Section: Introductionmentioning

confidence: 99%

The comparison of gut gene expression and bacterial community in Diaphorina citri (Hemiptera: Liviidae) adults fed on Murraya exotica and ‘Shatangju’ mandarin (Citrus reticulate cv. Shatangju)

et al. 2023

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Background Diaphorina citri Kuwayama is an important citrus pest. It serves as the vector for the transmission of Candidatus Liberibacter asiaticus (CLas), which induced a destructive disease, Huanglongbing, and caused huge economic losses. During the interaction between insects and plants, insects have evolved a series of mechanisms to adapt to various host plants. Murraya exotica and ‘Shatangju’ mandarin (Citrus reticulate cv. Shatangju) are the Rutaceae species from different genera that have been discovered as suitable hosts for D. citri adults. While the adaptation mechanism of this pest to these two host plants is unclear. Results In this study, RNA-seq and 16 S rDNA amplification sequencing were performed on the gut of D. citri adults reared on M. exotica and ‘Shatangju’ mandarin. RNA-seq results showed that a total of 964 differentially expressed genes were found in different gut groups with two host plant treatments. The impacted genes include those that encode ribosomal proteins, cathepsins, and mitochondrial respiratory chain complexes. According to 16 S rDNA sequencing, the compositions of the gut bacterial communities were altered by different treatments. The α and β diversity analyses confirmed that the host plant changes influenced the gut microbial diversity. The functional classification analysis by Tax4Fun revealed that 27 KEGG pathways, mostly those related to metabolism, including those for nucleotide metabolism, energy metabolism, metabolism of cofactors and vitamins, amino acid metabolism, carbohydrate metabolism, xenbiotics biodegradation and metabolism, lipid metabolism, and biosynthesis of other secondary metabolites, were significantly altered. Conclusion Our preliminary findings shed light on the connection between D. citri and host plants by showing that host plants alter the gene expression profiles and bacterial community composition of D. citri adults.

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Comparative microbiome analysis reveals bacterial communities associated with Candidatus Liberibacter asiaticus infection in the Huanglongbing insect vector Diaphorina citri

Cited by 6 publications

References 47 publications

Bacterial communities of the psyllid pest Bactericera cockerelli (Hemiptera: Triozidae) Central haplotype of tomato crops cultivated at different locations of Mexico

Bacterial communities of the psyllid pest Bactericera cockerelli (Hemiptera: Triozidae) Central haplotype of tomato crops cultivated at different locations of Mexico

Investigation of Gut Bacterial Communities of Asian Citrus Psyllid (Diaphorina citri) Reared on Different Host Plants

The comparison of gut gene expression and bacterial community in Diaphorina citri (Hemiptera: Liviidae) adults fed on Murraya exotica and ‘Shatangju’ mandarin (Citrus reticulate cv. Shatangju)

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