Comparative mitogenomics and phylogenetic analyses of the genus Menida (Hemiptera, Heteroptera, Pentatomidae)

Abstract

In order to explore the genetic diversity and phylogenetic relationship of the genus Menida Motschulsky, 1861 and reveal the molecular evolution of the family Pentatomidae, subfamily Pentatominae, complete mitochondrial genomes of three species of Menida were sequenced, and the phylogenetic relationships of tribes within the subfamily Pentatominae were studied based on these results. The mitochondrial genomes of Menida musiva (Jakovlev, 1876), M. lata Yang, 1934, and M. metallica Hsiao & Cheng, 1977 were 16,663 bp, 16,463 bp, and 16,418 bp, respectively, encoding 37 genes and including 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes, and a control region. The mitochondrial genome characteristics of Menida were compared and analyzed, and the phylogenetic tree of the Pentatominae was constructed based on the mitochondrial genome datasets using Bayesian inference (BI) and maximum likelihood (MI) methods. The results showed that gene arrangements, nucleotide composition, codon preference, gene overlaps, and RNA secondary structures were highly conserved within the Menida and had more similar characteristics in Pentatominae. The phylogenetic analysis shows a highly consistent topological structure based on BI and ML methods, which supported that the genus Menida belongs to the Pentatominae and is closely related to Hoplistoderini. The examined East Asian species of Menida form a monophyletic group with the internal relationships: (M. musiva + (M. lata + (M. violacea + M. metallica))). In addition, these results support the monophyly of Eysarcorini and Strachiini. Placosternum and Cappaeini are stable sister groups in the evolutionary branch of Pentatominae. The results of this study enrich the mitochondrial genome databases of Pentatominae and have significance for further elucidation of the phylogenetic relationships within the Pentatominae.

Describe the morphology and mitochondrial genome of Mecidea indica Dallas, 1851 (Hemiptera, Pentatomidae), with its phylogenetic position

“…typica was the first species to differentiate from Pentatomidae. These same results were obtained by Lian et al (2022) [ 66 ] and Ding et al (2023) [ 65 ]. Our study results rejected the monophyly of Pentatominae and Podopinae, and supported Asopinae as monophyletic.…”

“…indica using second-generation sequencing technology. The arrangement of the 37 genes was consistent with that of published Pentatomidae species [ 52 , 53 , 65 ], indicating that no gene rearrangements have occurred. The nucleotide composition of the mitochondrial genome of M .…”

“…In this study, we sequenced the complete mitochondrial genome of M. indica using secondgeneration sequencing technology. The arrangement of the 37 genes was consistent with that of published Pentatomidae species [52,53,65], indicating that no gene rearrangements have occurred. The nucleotide composition of the mitochondrial genome of M. indica exhibits high AT content, and base composition heterogeneity is common in Heteroptera species [49].…”

“…Our results agreed with those of Lian et al (2022) [66], who supported the monophyletic group of Phyllocephalinae. The monophyly of Eysarcorini and Strachiini is supported in many studies [24,53,65]. Halyini and Caystrini are closely related, forming a stable sister group relationship.…”

Evolutionary and phylogenetic insights from the mitochondrial genomic analysis of Diceraeus melacanthus and D. furcatus (Hemiptera: Pentatomidae)

“…In this study, AT (positive) and GC (negative) skews resemble the patterns normally reported for insects 29 , 91 – 93 . The length of concatenated PCGs of D. melacanthus (10,533 bp) was close to that of D. furcatus (10,351 bp), corresponding to approximately 61 and 67% of their mitogenomes, similarly to various Pentatomidae 14 , 29 , 70 , 82 , 94 . Nine PCGs ( nad2, cox1, cox2, atp8, atp6, cox3, nad3, nad6, cytb ) are encoded on the main strand and four on the complementary strand ( nad5, nad4, nad4L, nad1 ) (Fig.…”