Comparative Multiparametric Analysis of HeLa and RD Cell Culture Reactions to Solcoseryl

Aim:To evaluate the effectiveness of Solcoseryl on bone regeneration in rabbit calvarial bony defect through histological examination and histomorphometric analysis. Materials and Methods: Twenty New Zealand rabbits were divided into 4 groups. Two cavities were drilled in the calvarial bone of each rabbit. Defects were left empty in group A as a control group or filled with either Solcoseryl in group B, DM Bone in group C or (Solcoseryl & DM Bone) mixture in group D. Rabbits were sacrificed on day 14; the defects were removed and prepared for histological and histomorphometric analysis. The area percent of newly formed bone was estimated. Comparison of the results of all groups were statistically analyzed by ANOVA and Post-hoc tests. Results: Histological evaluation revealed presence of granulation tissues in control group while woven bone formation was evident in all experimental groups with evident osteoid tissue in group C & D. Histomorphometric analysis revealed that the quantity of newly formed bone was the greatest in mixture group. The greatest area percent of uncalcified bone was recorded in control. Conclusion: Combination of Solcoseryl and DM Bone has a synergistic effect on bone formation quantitatively and qualitatively.

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Determination of clinical efficiency of deproteinized dialysate from blood of milk-fed calves after endoprosthetics in patients with ventral hernias

Иванов

Викторович²,

Иванов

et al. 2020

I.P. Pavlov Russian Medical Biological Herald

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Aim. To study the influence of deproteinized dialysate from blood of milk-fed calves on the type of exudative discharge and dynamics of inflammatory reaction after hernia repair with plastics of the anterior abdominal wall with hernioendoprosthesis of polypropylene. Materials and Methods. The study involved 59 patients being on stationary treatment in the surgical department of Kursk Regional Clinical Hospital. Patients were hospitalized for the herniation of small or medium dimension. The patients were divided to two groups: the main (n=30) and control (n=29) groups. After endoprosthetics, the patients of the control group were given complex conservative treatment. The patients of the main group, besides standard treatment, were administered deproteinized dialysate from blood of milk-fed calves intravenously in drips 10 ml + 200 ml of 0.9% sodium chloride solution within 7 days. For cytological examination and determination of the type of cytograms, the traumatic discharge was collected and analyzed using the method of M.F. Kamaev and M.A. Palthsev. Results. Cytomorphometric examination was conducted on the third, fifth and seventh day after endoprosthetics with the aim of studying dynamic changes. Determination of cell composition and also of its changes characteristic of each studied period, was necessary for obtaining further information characterizing inflammatory process in the region of placement of the endoprosthesis. After endoprosthetics in patients who were administered hemodialysate, the inflammatory reaction was less pronounced than in patients who did not receive the preparation. This was associated with a more dynamic change of stages of the inflammatory process. In patients of the main group who received deproteinized dialysate, regenerative type of inflammation first appeared on the fifth day and made 6.9%; by the seventh day the share of patients with the regenerative type rose to 17.5%, while in the patients of the control group no regeneration stage was observed in both periods. Conclusion. Analysis of the efficiency of influence of hemodialysate on the inflammatory reaction in plastics of the anterior abdominal wall with polypropylene endoprosthesis evidences faster course of all stages of inflammation and reduction of its intensity by 10%.

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Comparative Multiparametric Analysis of HeLa and RD Cell Culture Reactions to Solcoseryl

Cited by 3 publications

References 6 publications

The Environmental Pollutant Bisphenol A Interferes with Nucleolar Structure

The Environmental Pollutant Bisphenol A Interferes with Nucleolar Structure

Evaluation of the Effect of Solcoseryl on Promotion of Bone Regeneration in Calvarial Bony Defect – An Experimental Pilot study

Determination of clinical efficiency of deproteinized dialysate from blood of milk-fed calves after endoprosthetics in patients with ventral hernias

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