Comparative nuclear magnetic resonance studies on water diffusional permeability of red blood cells from mice and rats

Benga, Gheorghe; Matei, Horea; Borza, Tudor; Poruţiu, Dorin; Lupşe, C.

doi:10.1016/0300-9629(93)90453-b

Cited by 24 publications

(18 citation statements)

References 24 publications

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“…The value of 2.1 · 10 À3 cm s À1 is similar to the previous results for human erythrocytes, such as by Stanisz et al [9] À2.8 · 10 À3 cm s À1 and Finkelstein [1] À3 · 10 À3 cm s À1 . It also falls well into the range of membrane permeability measured for different mammals [21,22,25,[55][56][57][58][59][60].…”

Section: Ddif Spectrummentioning

confidence: 83%

Study of diffusion in erythrocyte suspension using internal magnetic field inhomogeneity

Lisitza

Warren

Song

2007

Journal of Magnetic Resonance

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Section: Ddif Spectrummentioning

confidence: 83%

Study of diffusion in erythrocyte suspension using internal magnetic field inhomogeneity

Lisitza

Warren

Song

2007

Journal of Magnetic Resonance

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“…Subsequently, it was found that a significant fraction of the population of spins in the high-energy state exchanged across the membrane within the longitudinal relaxation time; hence the transmembrane exchange rate was able to be determined with a 1D-EXSY (12) experiment ( Figure 1). Figure 1A shows the fully relaxed 19 F NMR spectrum of FE in a suspension of RBCs. The separation between the intracellular and extracellular FE resonances (δν) was 69 Hz, or 0.18 ppm.…”

Section: Exchangementioning

confidence: 99%

“…An initial 19 F NMR experiment showed that FE became equilibrated between the intra-and extracellular compartments of RBC suspensions in the time it took to obtain the first spectrum, ∼2 min. Subsequently, it was found that a significant fraction of the population of spins in the high-energy state exchanged across the membrane within the longitudinal relaxation time; hence the transmembrane exchange rate was able to be determined with a 1D-EXSY (12) experiment ( Figure 1).…”

Section: Exchangementioning

confidence: 99%

“…Ammonia/ammonium (NH 4 + ), methylamine (MA), and ethylamine (EA) were added to suspensions of RBCs containing 8.0 mM FE and 8.0 mM 3FG, at 37°C. The rate constants for exchange of the FE and 3FG (18) were determined from 19 F NMR 1D-EXSY experiments. These measurements were also made in the absence of 3FG; no effect was seen on the exchange rate of FE in this situation (data not shown).…”

Section: ∆ψ )mentioning

confidence: 99%

“…We discovered that an organic amine, 2-fluoroethylamine (FE), which is readily detectable by 19 F nuclear magnetic resonance (NMR) spectroscopy, has a characteristic that enables measurement of its rapid transmembrane exchange. The exchange is measured under conditions whereby the solute is in electrochemical equilibrium across the membrane of the cell; the situation is called equilibrium exchange, and the NMR experiment to measure the transport is akin to adding a radioactive tracer to a solute-transport system that is already in electrochemical equilibrium.…”

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confidence: 99%

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Rapid Exchange of Fluoroethylamine via the Rhesus Complex in Human Erythrocytes: ¹⁹F NMR Magnetization Transfer Analysis Showing Competition by Ammonia and Ammonia Analogues

et al. 2006

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A remarkable recent discovery in red blood cell function is that the Rhesus antigen complex that for so long was considered to be simply a means of cell recognition is also the ammonia transporter. It catalyzes transmembrane exchange of ammonia on the subsecond time scale, and yet because of a lack of rapid-exchange methodology its kinetics had not been characterized. The flux of ammonia varies appreciably in diverse clinical states, and a convenient method for its characterization would be of basic and of clinical diagnostic value. Fluoroethylamine is water-soluble and when added to a suspension of human red blood cells (RBCs) displays the experimentally useful property of giving separate 19F NMR spectral peaks for the populations inside and outside the cells. By using two-site, one-dimensional magnetization exchange spectroscopy (1D-EXSY), the transmembrane exchange of fluoroethylamine was measured; it was found to occur on the subsecond time scale with an apparent first-order rate constant for efflux, under the equilibrium exchange conditions, of 3.4 s(-1). The method was used to characterize the concentration, temperature, and pH dependence of the exchange rate constant. We determined the extent of competitive inhibition exhibited by ammonia and two molecules that contain an amine group (ethylamine and methylamine). Inhibition of the exchange by incubating the suspension with anti-RhAG antibody, and no inhibition by anti-RhD antibody, suggested specificity of exchange via the RhAG protein of the Rh complex.

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Water channel proteins (later called aquaporins) and relatives: Past, present, and future

Benga

2009

IUBMB Life

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123

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SummaryWater channels or water channel proteins (WCPs) are transmembrane proteins that have a specific three-dimensional structure with a pore that can be permeated by water molecules. WCPs are large families (over 450 members) that are present in all kingdoms of life. The first WCP was discovered in the human red blood cell (RBC) membrane in 1980s. In 1990s other WCPs were discovered in plants, microorganisms, various animals, and humans; and it became obvious that the WCPs belong to the superfamily of major intrinsic proteins (MIPs, over 800 members). WCPs include three subfamilies: (a) aquaporins (AQPs), which are water specific (or selective water channels); (b) aquaglyceroporins (and glycerol facilitators), which are permeable to water and/or other small molecules; and (c) ''superaquaporins'' or subcellular AQPs. WCPs (and MIPs) have several structural characteristics which were better understood after the atomic structure of some MIPs was deciphered. The structure-function relationships of MIPs expressed in microorganisms (bacteria, archaea, yeast, and protozoa), plants, and some multicellular animal species [nematodes, insects, fishes, amphibians, mammals (and humans)] are described. A synthetic overview on the WCPs from RBCs from various species is provided. The physiological roles of WCPs in kidney, gastrointestinal system, respiratory apparatus, central nervous system, eye, adipose tissue, skin are described, and some implications of WCPs in various diseases are briefly presented. References of detailed reviews on each topic are given. This is the first review providing in a condensed form an overview of the whole WCP field that became in the last 20 years a very hot area of research in biochemistry and molecular cell biology, with wide and increasing implications.

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Comparative nuclear magnetic resonance studies on water diffusional permeability of red blood cells from mice and rats

Cited by 24 publications

References 24 publications

Study of diffusion in erythrocyte suspension using internal magnetic field inhomogeneity

Study of diffusion in erythrocyte suspension using internal magnetic field inhomogeneity

Rapid Exchange of Fluoroethylamine via the Rhesus Complex in Human Erythrocytes: ¹⁹F NMR Magnetization Transfer Analysis Showing Competition by Ammonia and Ammonia Analogues

Water channel proteins (later called aquaporins) and relatives: Past, present, and future

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Comparative nuclear magnetic resonance studies on water diffusional permeability of red blood cells from mice and rats

Cited by 24 publications

References 24 publications

Study of diffusion in erythrocyte suspension using internal magnetic field inhomogeneity

Study of diffusion in erythrocyte suspension using internal magnetic field inhomogeneity

Rapid Exchange of Fluoroethylamine via the Rhesus Complex in Human Erythrocytes: 19F NMR Magnetization Transfer Analysis Showing Competition by Ammonia and Ammonia Analogues

Water channel proteins (later called aquaporins) and relatives: Past, present, and future

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Rapid Exchange of Fluoroethylamine via the Rhesus Complex in Human Erythrocytes: ¹⁹F NMR Magnetization Transfer Analysis Showing Competition by Ammonia and Ammonia Analogues