Comparative ovicidal activity of Moringa oleifera leaf extracts on Fasciola gigantica eggs

Abstract

Background:Fasciolosis is an important zoonotic disease affecting the productive performance of farm animals in Egypt.Aim:The aim of the present study was comparing the ovicidal effect of different extracts as an alcoholic (Methanolic and Ethanolic) and aqueous Moringa oleifera leaf extracts on Fasciola gigantica non-embryonated and developed eggs.Materials and Methods:Tested concentrations of extracts ranged from 12.5 to 800 mg/ml. Nitroxynil was used as reference drug with a dose of 100 mg/ml.Results:M. oleifera alcoholic and aqueous extracts showed a concentration-dependent ovicidal effect on F. gigantica non-embryonated and developed eggs. Based on LC50 values, water extract showed the highest ovicidal activity since it registered the lowest values of 2.6 mg/ml on non-embryonated eggs. Non-embryonated eggs were more susceptible to aqueous extract than developed eggs. On the other hand, the developed eggs were more susceptible to ethanolic extract than non-embryonated eggs even the lowest LC50 (12.38 mg/ml).Conclusion:M. oleifera leaf extracts especially aqueous extract could be a promising step in the field of controlling fascioliasis. Further, in vivo studies are needed to enlighten the therapeutic potential of M. oleifera extracts in treating F. gigantica infection.

Cellular immune response and scanning electron microscopy in the evaluation of Moringa leaves aqueous extract effect on Cryptosporidium parvum in buffalo intestinal tissue explants

“…oleifera leaves were purchased from local market, Cairo, Egypt. Moringa leaves were dried in shade and extract was prepared according to Harpone (1984). In brief, 200 gm leaves were ground into powder, extracted in 2000 ml of distilled water with stirring, left for 24 h in cold conditions 4°C and filtered with sterile filter papers (Whattman No.1) then, dried under reduced pressure in a rotary evaporator at a temperature below 50°C and stored at -20°C until use.…”

Anticryptosporidial effect of pomegranate peels water extract in experimentally infected mice with special reference to some biochemical parameters and antioxidant activity

“…Many agents had been tested and there was no effective or approved treatment for cryptosporidiosis. In the scope of food safety and public health, plants and their compounds offered an alternative approach to treat parasitic diseases (Hegazi et al 2018). Some reports tested the effect of plant extracts in the treatment of cryptosporidiosis such as: garlic , cinnamon and onion (Abu El Ezz et al 2011) and pomegranate (Al-Mathal and Alsalem 2013).…”

Antibacterial efficacy of Moringa oleifera leaf extract against pyogenic bacteria isolated from a dromedary camel (Camelus dromedarius) abscess

“…According to the World Health Organization, 80% of the populations in developing countries prefer to use herbal extract and their active components as traditional medicine therapy[4]. M. oleifera is commonly known as drumstick and has many active components such as alkaloids, tannins, flavonoids, saponins, and triterpenoids [15,16] with potent anthelmintic activity [17] and antibacterial effect [18]. The phytochemical analysis of M. oleifera showed its bioactive compounds [19,20] with their pharmacological activity [21].…”