Comparative Pathogenicity of Aeromonas spp. in Cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus)

Azzam-Sayuti, Mohamad; Ina‐Salwany, Yasin; Zamri‐Saad, Mohd; Salleh, Annas; Yusof, Mohd Termizi; Monir, Shirajum; Mohamad, Aslah; Muhamad-Sofie, Mohd Hafiz Ngoo; Lee, Jing Yie; Chin, Yong Kit; Amir-Danial, Zahaludin; Asyiqin, Addenan; Basri, Lukman; Liles, Mark R.; Amal, Mohammad Noor Azmai

doi:10.3390/biology10111192

Cited by 25 publications

(17 citation statements)

References 46 publications

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“…farm practices can increase the susceptibility of fishes to motile Aeromonas septicemia (MAS) outbreak. However, polymorphic phenotypes and genotypes can lead to misidentification of Aeromonas isolates from diseased fish (AzzAM-SAyuTI et al, 2021). So, to achieve appropriate treatment for bacterial diseases, we should first accurately identify the bacterial species, for this, conventional microbiological methods used for the identification of pathogenic bacteria are very laborious and timeconsuming.…”

Section: Pellin Et Almentioning

confidence: 99%

“…Aeromonas bacteria can cause an infection characterized by septicemia and is one of the most common pathogens in tropical fish. This disease is responsible for high morbidity and mortality rates, causing considerable losses in aquaculture (MARInHO-nETO et al, 2019;AzzAM-SAyuTI et al, 2021), thus, the understanding of its pathophysiology is crucial to develop control strategies of this bacterial infection in farmed fish (MARInHO-nETO et al, 2019) Aeromonas species are ubiquitous in diverse aquatic habitats. High stocking density and improper RESUMO: O objetivo deste estudo foi identificar molecularmente diferentes espécies de Aeromonas isoladas de tambaqui (Colossoma macropomum) de cultivo do norte do Brasil e avaliar seu potencial patogênico pela presença de genes de virulência.…”

Section: Introductionmentioning

confidence: 99%

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Aeromonas from farmed tambaqui from North Brazil: molecular identification and pathogenic potential

et al. 2023

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The aim of this study was to molecularly identify different species of Aeromonas isolated from farmed tambaqui (Colossoma macropomum) from North Brazil, and evaluate their pathogenic potential by the presence of virulence genes. From the extraction of bacterial DNA, PCR (polymerase chain reaction) of the primers 16S rDNA, aerA (cytolytic enterotoxin), ast (cytotoxic enterotoxin) and act (cytotoxic enterotoxin) were performed. Of 24 isolates evaluated, eight amplified the ast gene, one amplified the act gene, but the areA gene was not amplified in any isolate. Sequencing of the 16S rRNA primer revealed a predominance of Aeromonas jandaei specie (92%). Aeromonas taiwanensis (4%), for the first time isolated from fish in Brazil, and Aeromonas hydrophila (4%) each appeared as just one isolate. Results showed that 32% of Aeromonas isolated from farmed tambaqui have considerable pathogenic potential for systemic damage, since the selected PCR primers are encoding the most common virulence genes in Aeromonas with high pathogenic intensity.

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Section: Pellin Et Almentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aeromonas from farmed tambaqui from North Brazil: molecular identification and pathogenic potential

et al. 2023

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“…The reason that the initial species richness decreased may be because (1) in this study, crawfish were vacuum packaged at 4 °C, and the SSOs that we detected belong to psychrophilic and facultative bacteria [ 36 , 37 , 38 ]. Anaerobic and low-temperature environments provide favorable conditions for the growth of SSOs.…”

Section: Resultsmentioning

confidence: 99%

The Quality and Bacterial Community Changes in Freshwater Crawfish Stored at 4 °C in Vacuum Packaging

Qiu

Zhao

et al. 2022

Molecules

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Crawfish can be easily spoiled due to their rich nutrition and high water content, which is difficult to preserve. In this study, the dominant spoilage organisms in crawfish which were stored at 4 °C in vacuum packaging were identified by high-throughput sequencing technology; after sequencing the full-length 16S rRNA gene, the changes in the bacterial community structure, diversity and quality (texture, flavor, etc.) were analyzed. Our results reflected that the specific spoilage organisms (SSOs) of crawfish were Aeromonas sobria, Shewanella putrefaciens, Trichococcus pasteurii and Enterococcus aquimarinus, since their abundances significantly increased after being stored for 12 days at 4 °C under vacuum conditions. At the same time, the abundance and diversity of the microbial community decreased with storage time, which was related to the rapid growth of the dominant spoilage organisms and the inhibition of other kinds of microorganisms at the end of the spoilage stage. Function prediction results showed that the gene which contributed to metabolism influenced the spoilage process. Moreover, the decline in texture of crawfish was negatively correlated to the richness of SSOs; this may be because SSOs can produce alkaline proteases to degrade the myofibrillar protein. On the contrary, the unpleasant flavor of crawfish, resulting from volatile flavor compounds such as S-containing compounds and APEOs, etc., is negatively correlated to the richness of SSOs, due to the metabolism of SSOs by secondary metabolites such as terpenoids, polyketides and lips, which can lead to decarboxylation, deamination and enzymatic oxidation. These results are very important to achieve the purpose of targeted inhibition of crawfish spoilage at 4 °C in vacuum packaging.

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“…Sectioning was done at 4 µm, and the tissues were stained with Hematoxylin and Eosin. These sections were examined and scored based on a previously described method [ 22 ]. Briefly, eight random fields were examined at magnifications 40× to 400×.…”

Section: Methodsmentioning

confidence: 99%

Evaluating the Intestinal Immunity of Asian Seabass (Lates calcarifer, Bloch 1790) following Field Vaccination Using a Feed-Based Oral Vaccine

et al. 2023

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This study describes the levels of gut lysozyme and IgM, the number, size and density of gut-associated lymphoid tissue (GALT) regions, and the lymphocyte population in Asian seabass following field oral administration of a feed-based vaccine. Fish in a grow-out farm were selected and divided into two groups; Group 1 was vaccinated at week 0, 2, and 6, while Group 2 was not vaccinated. Samplings were done at 2-week intervals when the fish were observed for clinical signs, and gross lesions were recorded. The intestinal tissue and gut lavage fluid were collected. GALT regions (numbers, size, density and population of lymphocytes) were analyzed. Clinical signs such as abnormal swimming pattern and death, and gross lesions including scale loss, ocular opacity, and skin ulceration were observed in both groups. At the end of the study, the incidence rate between both groups were significantly different (p < 0.05). The gut IgM level and lysozyme activity, lymphocyte population, number, size and density of GALT regions of Group 1 were significantly (p < 0.05) higher than Group 2. Therefore, this study concludes that the feed-based vaccine reduces the incidence of vibriosis by stimulating the gut immunity of the vaccinated fish with an enhanced GALT region, specific IgM production against Vibrio harveyi, and lysozyme responses.

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Comparative Pathogenicity of Aeromonas spp. in Cultured Red Hybrid Tilapia (Oreochromis niloticus × O. mossambicus)

Cited by 25 publications

References 46 publications

Aeromonas from farmed tambaqui from North Brazil: molecular identification and pathogenic potential

Aeromonas from farmed tambaqui from North Brazil: molecular identification and pathogenic potential

The Quality and Bacterial Community Changes in Freshwater Crawfish Stored at 4 °C in Vacuum Packaging

Evaluating the Intestinal Immunity of Asian Seabass (Lates calcarifer, Bloch 1790) following Field Vaccination Using a Feed-Based Oral Vaccine

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