Comparative Peptidomics Analysis of Fermented Milk by Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus delbrueckii ssp. lactis

Ye, Hongji; Zhang, Xinyi; Jiang, Yang; Guo, Min; Liu, Xiaoming; Zhao, Jianxin; Zhang, Hao; Chen, Wei

doi:10.3390/foods10123028

Cited by 8 publications

(4 citation statements)

References 32 publications

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“…This high metabolic property enhances the fermentation ability of these strains with the production of essential metabolites (e.g., bioactive peptides, lactate, SCFA, and vitamins). These compound’s synthesis requires specific enzymes (e.g., proteinases and peptidases, glyceraldehyde-3-phosphate dehydrogenase, pyruvate kinase, phosphoketolase, acetate kinase, lactate dehydrogenase, riboflavin kinase, thiamine pyrophosphokinase, among others) of these strains, involved in proteolysis, and both phosphoketolase or Embden-Meyerhof (EMP) metabolic pathways ( Kandler, 1983 ; Ye et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Genomic Characterization of Lactobacillus delbrueckii Strains with Probiotics Properties

et al. 2022

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Probiotics are health-beneficial microorganisms with mainly immunomodulatory and anti-inflammatory properties. Lactobacillus delbrueckii species is a common bacteria used in the dairy industry, and their benefits to hosting health have been reported. This study analyzed the core genome of nine strains of L. delbrueckii species with documented probiotic properties, focusing on genes related to their host health benefits. For this, a combined methodology including several software and databases (BPGA, SPAAN, BAGEL4, BioCyc, KEEG, and InterSPPI) was used to predict the most important characteristics related to L. delbrueckii strains probiose. Comparative genomics analyses revealed that L. delbrueckii probiotic strains shared essential genes related to acid and bile stress response and antimicrobial activity. Other standard features shared by these strains are surface layer proteins and extracellular proteins-encoding genes, with high adhesion profiles that interacted with human proteins of the inflammatory signaling pathways (TLR2/4-MAPK, TLR2/4-NF-κB, and NOD-like receptors). Among these, the PrtB serine protease appears to be a strong candidate responsible for the anti-inflammatory properties reported for these strains. Furthermore, genes with high proteolytic and metabolic activity able to produce beneficial metabolites, such as acetate, bioactive peptides, and B-complex vitamins were also identified. These findings suggest that these proteins can be essential in biological mechanisms related to probiotics’ beneficial effects of these strains in the host.

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Section: Discussionmentioning

confidence: 99%

Genomic Characterization of Lactobacillus delbrueckii Strains with Probiotics Properties

et al. 2022

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“…A method previously described [ 19 ] was followed with minor modification. Samples were analyzed using an EASY nLC 1200 system equipped with an Acclaim PepMapTM RSLC (50 μm × 15 cm, 2 μm, 100 Å).…”

Section: Methodsmentioning

confidence: 99%

Comparative Peptidomics Analysis of Milk Fermented by Lactobacillus helveticus

Gao

Jiang

Zhang

et al. 2022

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Lactobacillus helveticus is one of the commonly used starter cultures for manufacturing various fermented dairy products. However, only a few studies have explored the cleavage region preference of L. helveticus with different cell envelope proteinase (CEP) genes. In the present study, we profiled the peptide composition of milk samples fermented by three different L. helveticus strains by means of peptidomics to illustrate their different proteolysis patterns. The result revealed that the differences in peptide profiles of milk samples fermented by different L. helveticus strains were mainly a result of variations in the peptide patterns of the casein fractions, which were correlated with CEP genotypes. This was mainly reflected in the extensiveness of the hydrolysis region of αS1-casein and the degree of β-casein hydrolysis. Bioactive peptides were mostly derived from the hydrolysis region common to the three L. helveticus strains, and DQHXN-Q32M42 fermentation resulted in the highest diversity and abundance of bioactive peptides and a significant antihypertensive effect in spontaneous hypertension rats.

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“…Ye et al (2021) [ 4 ] reported the peptidomics of milk fermented by Lactobacillus delbrueckii both before and after the simulated gastrointestinal digestion. The peptides derived from β-casein were abundant in the samples of L. delbreuckii ssp.…”

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confidence: 99%