Comparative Performance of Herpes Simplex Virus Type 2-Specific Serologic Assays from Meridian Diagnostics and MRL Diagnostics

Ribes, Julie A.; Hayes, Marie; Smith, Angela; Winters, Jeffrey L.; Baker, Doris J.

doi:10.1128/jcm.39.10.3740-3742.2001

Cited by 31 publications

(21 citation statements)

References 17 publications

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“…These false positives were attributed to an antibody cross-reactivity to glycoprotein G1 that lacked any HSV common antigen specificity. A similar type of cross-reactivity has been noted in our laboratory for the MRL HSV-2 type-specific EIA (12). The majority of specimens seen as false positives with the MRL assay produced EIA values just above the equivocal range.…”

supporting

confidence: 76%

Comparative Performance of Herpes Simplex Virus Type 1-Specific Serologic Assays from MRL and Meridian Diagnostics

Ribes

Smith

Hayes³

et al. 2002

J Clin Microbiol

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Two companies, MRL and Meridian Diagnostics, have developed Food and Drug Administration-approved herpes simplex virus type 1 type-specific enzyme immunoassays. The sensitivity, specificity, and overall testing efficiency of these assays were 98.2, 93.8, and 96.6% for MRL and 98.8, 99.0, and 98.1% for Meridian, making both of these kits suitable for use in the clinical lab.The herpes simplex viruses (herpes simplex virus type 1 [HSV-1] and HSV-2) are important causes of disease worldwide. HSV-1 is the primary cause of oral-facial and pharyngeal herpes infections and may cause herpetic whitlow, eye infections, and disseminated disease (1,2,8,15). HSV-1 also accounts for 10 to 15% of all genital herpetic infections (3, 6, 11). The proportion of genital infections with HSV-1 is reportedly even higher in some locations (4, 10, 13).Transmission of HSV-1 occurs following contact with fluid from vesicular lesions or contact with infected body fluids, such as saliva and genital secretions (4,8,9). Although HSV-1 has historically been acquired primarily in childhood, acquisition of the virus is now often seen during early adulthood (4,8,9). Seroprevalence studies from several sites worldwide have indicated that approximately 60 to 70% of adolescents have not developed antibodies to this virus and are therefore still susceptible to infection with HSV-1 (5, 7, 8).HSV-1 serostatus is also important with regard to the vaccine developed for HSV-2. The usefulness of the vaccine appears to be linked to the HSV-1 serostatus of the individual being immunized. Only female recipients with a negative HSV-1 serostatus prior to immunization appear to derive benefit from this vaccine (S. Spruance and The Herpes Vaccine Efficacy Study Group, Abstr. Addendum 40th Intersci. Conf. Antimicrob. Agents Chemother., abstr. L-6, p. 22, 2000). This, coupled with the role of HSV-1 as a significant etiologic agent for herpes genitalis, produces the need for a reliable HSV-1 immunoglobulin G (IgG) type-specific assay. This report focuses on the head-to-head comparison of two Food and Drug Administration (FDA)-approved HSV-1 type-specific EIAs.Serum from 532 blood donor specimens was collected from the Central Kentucky Blood Center, Lexington, Ky., and frozen in 2-ml aliquots at Ϫ70°C until testing. Serologic evaluation of HSV-1 antibodies was performed using glycoprotein G type-specific enzyme immunoassays from Meridian Diagnostics Inc. (Cincinnati, Ohio) and from MRL Diagnostics (Cypress, Calif.). All testing was performed according to the manufacturers' specifications. Absorbances were read on an automated ELx800 Universal Microplate Reader (Bio-Tek Instruments Inc.) at 405 nm for the Meridian assays and 450 nm for the MRL assays. For both manufacturers, absorbency cutoff values were those established by validation studies with a mean absorbency value: those with Ͼ0.99 times the reference absorbency were interpreted as positive, those with 0.91 to 0.99 times the reference absorption were interpreted as equivocal, and those with less than 0.91 time...

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supporting

confidence: 76%

Comparative Performance of Herpes Simplex Virus Type 1-Specific Serologic Assays from MRL and Meridian Diagnostics

Ribes

Smith

Hayes³

et al. 2002

J Clin Microbiol

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“…Herpes simplex-2 plasma antibodies The presence of type-specific plasma IgG antibodies against HSV-2 (anti-HSV-2) was tested using an HSV-2 ELISA assay developed by Focus Technology/ formerly MRL (Cypress, California) (Ribes et al, 2001). All HSV-2 positive findings were confirmed by means of a second test.…”

Section: Human Papillomavirus and Herpes Simplex-2 (Hsv-2) Detection mentioning

confidence: 99%

Prevalence of papillomavirus infection in women in Ibadan, Nigeria: a population-based study

et al. 2004

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To investigate the prevalence of and the risk factors for cervical infection with human papillomavirus (HPV) in an inner-city area of Ibadan, Nigeria, we interviewed and obtained a sample of cervical cells from 932 sexually active women aged 15 years or older. A total of 32 different HPV types were identified with an HPV prevalence of 26.3% overall and 24.8% among women without cervical lesions; or age-standardised to the world standard population of 28.3 and 27.3%, respectively. High-risk HPV types predominated, most notably HPV 16, 31, 35 and 58. In all, 33.5% of infections involved more than one HPV type. Unlike most populations studied so far, HPV prevalence was high not only among young women, but also in middle and old age. Single women (odds ratio, OR ¼ 2.1; 95% confidence interval, CI ¼ 1.1 -3.9) and illiterate women (OR ¼ 1.7; 95%CI ¼ 1.1 -2.5) showed increased HPV positivity. Associations were also found with anti-Herpes simplex-2 antibodies (OR ¼ 1.6; 95% CI: 1.1 -2.1) and with the husband's extramarital relationships (OR ¼ 1.6: 95% CI: 1.0 -2.6). High prevalence of HPV in all age groups may be a distinctive feature of populations where HPV transmission continues into middle age and cervical cancer incidence is very high.

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“…Serologic testing was conducted blindly in a central laboratory in Seattle, Washington, using a HSV-2 ELISA assay developed by Focus Technology (formerly MRL, Cypress, CA; ref. 26). All HSV-2-positive sera were retested to confirm results.…”

Section: Introductionmentioning

confidence: 99%

Sexual Behavior, Condom Use, and Human Papillomavirus: Pooled Analysis of the IARC Human Papillomavirus Prevalence Surveys

Vaccarella

Franceschi

Herrero

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

167

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Human papillomavirus (HPV) is a sexually transmitted infection but it is unclear whether differences in transmission efficacy exist between individual HPV types. Information on sexual behavior was collected from 11 areas in four continents among population-based, age-stratified random samples of women of ages z15 years. HPV testing was done using PCRbased enzyme immunoassay. Unconditional logistic regression was used to estimate odds ratios (OR) of being HPV positive and corresponding 95% confidence intervals (95% CI). Variables were analyzed categorically. When more than two groups were compared, floating confidence intervals were estimated by treating ORs as floating absolute risks. A total of 11,337 women (mean age, 41.9 years) were available. We confirmed that lifetime number of sexual partners is associated with HPV positivity (OR for z2 versus 1, 1.86; 95% CI, 1.63-2.11) but the association was not a linear one for HPV18, 31, and 33 (i.e., no clear increase for z3 versus 2 sexual partners). Women who had multiple-type infection and highrisk HPV type infection reported a statistically nonsignificant higher number of sexual partners than women who had single-type and low-risk type infections, respectively. Early age at sexual debut was not significantly related to HPV positivity. Husband's extramarital sexual relationships were associated with an OR of 1.45 (95% CI, 1.24-1.70) for HPV positivity in their wives after adjustment for age and lifetime number of women's sexual partners. We did not observe a significant association with condom use. Our study showed an effect of both women's and their husbands' sexual behavior on HPV positivity. Furthermore, it suggests some differences in the pattern of the association between sexual behavior and different HPV types. (Cancer Epidemiol Biomarkers Prev 2006;15(2):326 -33)

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Comparative Performance of Herpes Simplex Virus Type 2-Specific Serologic Assays from Meridian Diagnostics and MRL Diagnostics

Cited by 31 publications

References 17 publications

Comparative Performance of Herpes Simplex Virus Type 1-Specific Serologic Assays from MRL and Meridian Diagnostics

Comparative Performance of Herpes Simplex Virus Type 1-Specific Serologic Assays from MRL and Meridian Diagnostics

Prevalence of papillomavirus infection in women in Ibadan, Nigeria: a population-based study

Sexual Behavior, Condom Use, and Human Papillomavirus: Pooled Analysis of the IARC Human Papillomavirus Prevalence Surveys

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