Comparative Phytochemical Analyses and in-vitro Antioxidant Activity of Aqueous and Ethanol Extracts of Simarouba glauca (Paradise Tree)

Osagie-Eweka, S. D. E.; Orhue, Noghayin E. Jerry; Ekhaguosa, Diamond

doi:10.9734/ejmp/2016/24736

Cited by 12 publications

(16 citation statements)

References 21 publications

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“…It was observed that the methanol extract remarkably demonstrated a high reducing potential, tracked by aqueous and ethanol extracts, and lastly by the control BHT with IC 50 4.70, 4.80, 4.90 and 5.00 μg, respectively. The results of the study is in line with the report of Osagie-Eweka et al (2016); although, they neither reported studies on stem bark nor methanol extracts, but of aqueous and ethanol leaf extracts of S. glauca. The results of the present study, however disagrees with the report of Parul et al (2013), obviously due to the difference in plants applied and methodology.…”

Section: Total Antioxidant Activity (Frap)supporting

confidence: 89%

“…The parts of the plant commonly reported to be used locally are the leaves, wood and stem back (Technical Data Report for Simarouba, 2002). The leaf extracts of S. glauca have previously been reported to possess some essential phytochemicals and anti-free radical potentials (Osagie-Eweka et al, 2016;Umesh, 2015). On the bases of the previous research conducted, further studies of thephytochemicals and anti-free radical potential of the stem bark extracts of S. glauca was done.…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical analyses and comparative in vitro antioxidant studies of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca DC. (Paradise tree)

Osagie-Eweka¹

2018

Afr. J. Plant Sci.

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This study was conducted to evaluate selected phytochemicals (antioxidant potentials of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca) relative to standard antioxidants. Sample was harvested, air dried, pulverized and extracted with aqueous and absolute methanol and ethanol; freeze dried at the National Energy Commission Centre, University of Benin. Alkaloids, phenols and tannins were identified, a n d also flavonoid was detected. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, reducing power activity, total antioxidant activity, hydroxyl radical activity, trolox equivalent antioxidant activity and nitric oxide (NO • ) radical scavenging activity of stem bark extracts of S. glauca were evaluated, and all t h e experiment were conducted in a dose dependent manner. Butylated hydroxytuolene (BHT), ascorbate and trolox were introduced as positive controls (antioxidant). DPPH radical scavenging activity of stem bark extracts did not exhibit anti-radical activity at 50% inhibition but demonstrated less anti-radical activity at percentage inhibition lower than 50%. Extracts yielded significant reducing power and total antioxidant activities (FRAP). Hydroxyl radical scavenging activities of extracts was substantial; extracts exhibited significant anti-radical activity when trolox was introduced as positive antioxidant control, while nitric oxide scavenging activities was unprecedented. The presence of phytochemicals and antioxidant principles proffer high medicinal value to S. glauca.

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Section: Total Antioxidant Activity (Frap)supporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Phytochemical analyses and comparative in vitro antioxidant studies of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca DC. (Paradise tree)

Osagie-Eweka¹

2018

Afr. J. Plant Sci.

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“…The antioxidant activity of these extracts was measured using the assays: DPPH, hydrogen peroxide, reducing power, and NO. Researchers in the past have explored the phytochemical constituents and antioxidant activity in the leaf extracts of S. glauca [38][39][40]. The in vitro studies have further demonstrated the physiological properties of the leaf extract [39].…”

Section: No Radical Scavenging Assaymentioning

confidence: 99%

Comparative Phytochemical Profile and Antioxidant Property of Bark, Flowers and Leaves Extracts of Simarouba Glauca

Sajeeda

et al. 2019

Asian J Pharm Clin Res

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Objective: The current study was to evaluate and compare the phytochemical constituents and antioxidant activity of bark, flowers and leaves of the tree Simarouba glauca. Methods: The solvent extraction of phytochemicals was carried out using Soxhlet apparatus with ethanol, chloroform, methanol, and water. The antioxidant property was determined by 2,2-Diphenyl-1-picrylhydrazyl, hydrogen peroxide free radical scavenging, reducing power assay, and nitric oxide radical scavenging assay using gallic acid and ascorbic acid as the standards. Results: The extraction yield was found maximum in the water extract of flower (3.7% w/w). Qualitative and quantitative analysis of phytoconstituents showed that the highest amount of alkaloids and flavonoid content (2.1% w/w) and (3.9% w/w), respectively, was in the chloroform extract of the flower. Phenol and carbohydrate constituent was found to be highest in the methanol extract of leaves 2.5% w/w and 2.2% w/w, respectively. The antioxidant assays showed that the bark possessed maximum antioxidant activity. The water extracts of S. glauca bark exhibited scavenging property (90%) with an IC50 value 39.63 μg/ml, and the least activity (56%) was observed in the methanol extracts of leaves with an IC50 value of 62.96 μg/ml of S. glauca. Conclusion: The study concluded that the water extract of the bark is a potent antioxidant compared to leaves and flowers. Further, in vivo studies are essential to enumerate its medicinal use and prove its efficacy in therapeutic applications.

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“…Simarouba glauca DC is rain-fed wasteland tree commonly called as ‘Laxmitaru’ or ‘Paradise tree’ belonging to the family Simaroubaceae. Different parts of the plant are being used traditionally as, antifungal, antibacterial, antioxidant ( Osagie-Eweka et al., 2016 ; Santhosh et al., 2016 ; Umesh, 2015 ), antiparasitic, antipyretic and anticancer agents ( Jose et al., 2019 ; Manasi and Gaikwad, 2011 ). The bark extract of this tree is commonly used for dysentery hence the bark is also called ‘dysentery-bark’ ( Santhosh et al., 2016 ; Manasi and Gaikwad, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Alpha glucosidase inhibition activity of phenolic fraction from Simarouba glauca: An in-vitro, in-silico and kinetic study

Mugaranja

Kulal

2020

Heliyon

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A phenolic rich fraction purified from Simarouba glauca leaves was effective in alpha glucosidase inhibition. The purified fraction named ‘fraction-14’ had shown significant inhibition of yeast alpha glucosidase enzyme activity (IC 50 = 2.4 ± 0.4 μg/mL) when compared to anti-diabetic drug acarbose (IC 50 = 2450 ± 24 μg/mL). The purified fraction also had reasonable DPPH (IC 50 = 14.4 ± 0.1 μg/mL) and ABTS (IC 50 = 7.6 ± 0.5 μg/mL) free radical scavenging activity when compared to the standard ascorbic acid. The LC-MS analysis of bioactive ‘fraction-14’ revealed four compounds, eclalbasaponin-v ( 1 ), cyanidin-3-O-(2′galloyl)-galactoside ( 2 ), kaempferol-3-O-glucoside ( 3 ) and kaempferol-3-O-pentoside ( 4 ) for the first time in S. glauca in this study. The kinetic study of the ‘fraction-14’ indicates a mixed type of inhibition on the alpha glucosidase enzyme with K i , 6.2 μg/mL. Docking studies showed promising binding energy for the compounds 2 (-7.769 kJ/mol), 3 (-7.04 kJ/mol) and 4 (-7.127 kJ/mol) against yeast alpha glucosidase which was better than acarbose (-6.867 kJ/mol). In conclusion, the phenolic rich fraction from S. glauca possessing good in-vitro antioxidant property and alpha glucosidase enzyme inhibition potential along with mixed inhibition kinetics. Also, better binding energy of compounds ( 1, 2 & 3 ) appears to contain potential lead-molecule for antidiabetic therapy.

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Comparative Phytochemical Analyses and in-vitro Antioxidant Activity of Aqueous and Ethanol Extracts of Simarouba glauca (Paradise Tree)

Cited by 12 publications

References 21 publications

Phytochemical analyses and comparative in vitro antioxidant studies of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca DC. (Paradise tree)

Phytochemical analyses and comparative in vitro antioxidant studies of aqueous, methanol and ethanol stem bark extracts of Simarouba glauca DC. (Paradise tree)

Comparative Phytochemical Profile and Antioxidant Property of Bark, Flowers and Leaves Extracts of Simarouba Glauca

Alpha glucosidase inhibition activity of phenolic fraction from Simarouba glauca: An in-vitro, in-silico and kinetic study

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