Comparative Phytochemical Analysis and Antioxidant Activities of Tamalakyadi Decoction with Its Modified Dosage Forms

Abstract

Background and Objective. Tamalakyadi decoction (TD) is a classical formulation mentioned in authentic traditional medicine text Sarasankshepaya under nasal diseases and used as a remedy for allergic rhinitis. It consists of 12 plant ingredients. Decoction preparations are widely used in Sri Lankan traditional system and considered effective and safe for treating many disorders. However, decoctions have to be used only in fresh state due to shorter shelf life and loss of stability. This decoction preparation method leads to decreasing the patient compliance and is also time consuming. Hence, the objective of the present study was to convert TD to consumer friendly novel dosage form, namely, freeze dried, spray dried, and traditional ganasara forms. Methodology. Therefore, we compared the phytochemical constituents and antioxidant activities of TD with its modified dosage forms. The chemical comparison of four dosage forms comprises phytochemical screening, TLC and HPTLC fingerprint profiles and the antioxidant activities by DPPH free radical scavenging activity, Ferric reducing antioxidant power (FRAP), total polyphenol content (TPC), and total flavonoid content (TFC). Results. Phytochemical screening revealed the presence of alkaloids, saponins, tannins, steroids, flavonoids, phenols, and terpenoids in all dosage forms. However, the saponins, alkaloids, flavonoids, terpenoids, and steroids were more prominent in TD and freeze dried preparation than the other two preparations. HPTLC fingerprint pattern of freeze dried dosage was more similar with HPTLC fingerprint pattern of TD in terms of number of peaks and their intensity compared to that of spray dried and ganasara dosage forms. Antioxidant activities such as DPPH, FRAP, TPC, and TFC were higher in decoction and freeze dried preparation than in spray dried and ganasara preparation. Conclusion. Freeze dried TD is the most suitable ready to use preparation having similar chemical properties and antioxidant activities to TD.

A Comparative Analytical Study on Two Types of Sharibadi Decoctions: An Ayurveda Preparation

“…TLC is one of the simple and cheap techniques available to detect phytochemical profiles in herbal drugs [6,13] or plants [4,15,16]. Differences in phytochemical constituents were revealed when compared the TLC fingerprint profile of Sharibadi decoction A with that of Sharibadi decoction B (Table 2 and Fig.…”

“…In brief, 20 g from each Sharibadi decoction A and B were taken into separate round bottoms and refluxed with distilled water (100°C) for 4 h and filtered. Each filtrate was subjected to phytochemical screening using standard protocols [5,6] with some modifications.…”

Efficacy and safety of two Ayurvedic dosage forms for allergic rhinitis: Study protocol for an open-label randomized controlled trial

“…This reconstituted powder also contains the above-mentioned 12 ingredients in almost the same quantities. In order to develop a ready-to-use formulation of the TMD 12, with composition similar to the TMD12 after preparation, three different formulations were developed and analyzed [12]. These included freeze-dried formulation, spray-dried formulation, and Ganasara formulation and detailed physicochemical and phytochemical analysis were performed.…”

“…These included freeze-dried formulation, spray-dried formulation, and Ganasara formulation and detailed physicochemical and phytochemical analysis were performed. The results are now published and showed that the freeze-dried formulation is quantitatively and qualitatively closest to TMD 12 [12]. Therefore the freeze-dried formulation was selected for evaluation of clinical efficacy and safety in this clinical trial.…”

Efficacy and safety of two Ayurvedic dosage forms for Allergic rhinitis: Study protocol for an open-label randomized controlled trial

“…This reconstituted powder also contains the above mentioned 12 ingredients in almost the same quantities. In order to develop a ready to use formulation of the TMD 12, with composition similar to the TMD12 after preparation, three different formulations were developed and analyzed [9]. These included freeze dried formulation, spray dried formulation and Gnanasara formulation and detailed physicochemical and phytochemical analysis were performed.…”