Comparative Phytochemical Profiles and Antioxidant Enzyme Activity Analyses of the Southern Highbush Blueberry (Vaccinium corymbosum) at Different Developmental Stages

Sun, Yueting; Li, Min; Mitra, Sangeeta; Muhammad, Rizwan Hafiz; Debnath, Biswojit; Lu, Xiaocao; Jian, Hongxiang; Qiu, Dongliang

doi:10.3390/molecules23092209

Cited by 62 publications

(38 citation statements)

References 44 publications

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“…Flavonoids are a big class of polyphenolic plant secondary metabolites, including flavonols, flavones, isoflavones, anthocyanins and so on [1]. Most of the flavonoids are effective natural antioxidants [2]. In human beings, flavonoids play important roles in maintaining normal vascular permeability and protecting against diseases such as hyperglycemia, cancer, and diabetes [3].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Antioxidant Activity and Flavonoids Metabolites in Peel and Flesh of Red-Fleshed Apple Varieties

Zhang

Xu³

et al. 2020

Molecules

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In this research, we compared the phenotypical characters, total anthocyanins content, total phenols content, and antioxidant activity of red-fleshed apple cultivars 'XJ4', 'QN-5', 'DH' and 'HX1' at three fruit developmental stages. A further flavonoids metabolites study was conducted in 'XJ4' and 'DH'. We found broader variation of total anthocyanins content in the peel of the four cultivars, which might result in larger differences of free radicals scavenging rate. The most significant difference in fruit phenotype, anthocyanins content, and DPPH scavenging rate was observed between 'XJ4' and 'DH' at mature stage. Therefore, the flavonoids metabolites of 'XJ4' and 'DH' at mature stage were compared to unveil the details of anthocyanins compounds. The unique compounds pelargonidin 3-O-β-d-glucoside and cyanidin-3-O-malonylhexoside were detected only in peel and flesh of 'XJ4' but not in 'DH', which might contribute to the purple peel and dark-red flesh color of 'XJ4'. Significantly decreased upstream metabolites in the early biosynthetic genes regulated domain were found only in 'XJ4' peel but not in the flesh. This might explain why the anthocyanins content in 'XJ4' peel was decreased largely at the mature stage. Taken together, our findings will give some insight into the metabolites study in flavonoid biosynthetic pathway of red-fleshed apple. Molecules 2020, 25, 0 2 of 22The number of hydroxyl groups on two aromatic benzene rings and the double bond positions on oxygenated heterocycle have a great impact on the sensitivity of anthocyanins to oxidation [9].As strong antioxidants, anthocyanins have a strong DPPH, ·OH, and O 2 ·radicals scavenging ability and the related studies have been intensively reported in many horticultural crops [10]. Previous study found the extract of anthocyanins from purple cabbage had strong capacity to scavenge free radicals [11]. Lyophilized pomegranate anthocyanins extract had strong ability to scavenge hydroxyl and superoxide anions [12]. Purple cauliflower had high total anthocyanins contents and scavenging rate of DPPH radical [13]. Red-fleshed apples (Malussieversii f. Neidzwetzkyana (Dieck) Langenf) are valuable resources attracting much more attention because they contain extremely high levels of anthocyanins compared to white and off-white fleshed apples [14]. The enriched anthocyanins properties of red-fleshed apple made it show stronger antioxidant activity. Our previous study indicated red-fleshed apple cultivar 'QN-5' exhibited stronger capacity for DPPH, OH, and O 2 ·radicals scavenging when compared to vitamin C [15]. A recent study found red-fleshed apples can inhibit the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells through studying the antioxidant and anti-proliferation properties, compared with a traditional cultivar 'Fuji' [16]. In addition, it's reported anthocyanins extract from red-fleshed apple could eliminate ROS induced oxidative damage in porcine cells [17]. Interestly, it has been found that the antioxidant activity and phenolic conten...

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Section: Introductionmentioning

confidence: 99%

Analysis of Antioxidant Activity and Flavonoids Metabolites in Peel and Flesh of Red-Fleshed Apple Varieties

Zhang

Xu³

et al. 2020

Molecules

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“…The SOD activity showed a positive correlation with the POD activity; both these enzyme activities showed a negative correlation with the PPO activity. Superoxide dismutase is an important antioxidant enzyme that eliminates free oxygen radicals in cells, and POD plays an important role in enhancing antioxidant capacity, which promotes the oxidation of phenolics [23]. Barley grass showed relatively high SOD and POD activities in the jointing stage.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, the three developmental stage groups were clearly separated by the two principal component axes, PC1 and PC2, which suggested that the compounds in barley grasses at three developmental stages could be clearly distinguished. The changes in the antioxidant enzyme activities among the different developmental stages are attributable to the transformation of chemical substances and the interaction of antioxidant enzymes [23]. The metabolism and antioxidant activity have been determined by phytochemical profiles and the enzyme activity through complex interactions at the different developmental stages of barley grasses, rather than simply being caused by the change of certain material [23].…”

Section: Discussionmentioning

confidence: 99%

“…The changes in the antioxidant enzyme activities among the different developmental stages are attributable to the transformation of chemical substances and the interaction of antioxidant enzymes [23]. The metabolism and antioxidant activity have been determined by phytochemical profiles and the enzyme activity through complex interactions at the different developmental stages of barley grasses, rather than simply being caused by the change of certain material [23]. However, the mechanisms underlying the antioxidant activity of the chemical constituents and enzymes isolated from barley grass need to be further investigated.…”

Section: Discussionmentioning

confidence: 99%

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Phytochemical Constituents and Antioxidant Enzyme Activity Profiles of Different Barley (Hordeum Vulgare L.) Cultivars at Different Developmental Stages

et al. 2019

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Barley grass possesses high nutritional value and antioxidant properties. In this study, the phytochemical constituents and antioxidant enzyme activities in six cultivars of barley grass were explored at three developmental stages: tillering, jointing, and booting stages. Total chlorophyll (Chl t) and carotenoid (Car) content, chlorophyll a/b (Chl a/b) ratio, total nitrogen nutrition (TNN), and total soluble protein (TSP) content, and superoxide dismutase (SOD), peroxidase (POD), and polyphenol oxidase (PPO) activities were assayed. The results indicated that the cultivar × development interaction was significant and that developmental stage was the main factor affecting the parameters studied. Cultivars had a negligible effect on these parameters, which varied with the developmental stages. In the tillering stage, Chl t and Car content, TNN, and POD activity achieved their highest value; in the jointing stage, SOD activity peaked; in the booting stage, Chl a/b ratio, TSP content, and PPO activity showed their highest values. TNN showed a negative correlation with TSP. Compared with those in the jointing, Chl t, Car, TSP, TNN content, Chl a/b ratio, and POD and PPO activities increased in the booting and the tillering stages, whereas SOD activity decreased. The differences in phytochemical constituents and antioxidant enzyme activities in barley grass were mainly correlated with the developmental stages. The aim of this study was to demonstrate the influence of developmental stages of barley grass on its phytochemical profile and antioxidant activities. Our results will help understand the mechanism of action of barley grass and provide theoretical support for the therapeutic application of barley grass.

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“…By contrast, Castrejón et al found that polyphenolic compounds' concentration and antioxidant activity in highbush blueberry fruits decreased from unripe green to ripe blue stages of fruit maturity [67]. Total flavonoids and vitamin C levels in blueberry fruits were also decreased as the fruits grow [68]. Those antioxidants in green fruits were highest and blue fruits were lowest.…”

Section: Degree Of Ripenessmentioning

confidence: 94%

Soil Preparation, Running Highbush Blueberry (Vaccinium corymbosum L.) Plantation and Biological Properties of Fruits

Zydlik¹,

Cieśliński²,

Chung³

et al. 2020

Modern Fruit Industry

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Due to the pro-health properties of highbush blueberry fruit, the interest in cultivation of this species has been growing significantly, which is evidenced by the current increase in world fruit production. Therefore, the aim of our review study is to present the impact of soil and climatic conditions and cultivation methods of Vaccinium corymbosum L. on fruit yield and quality in Central and Eastern Europe. In this region, one of the most important abiotic factors determining the yield level is the minimum temperature of the winter period and short-term increases in temperature, which are conducive to the damage to flower buds. Another factor determining the success of cultivation is soil. In addition, highbush blueberry has specific soil requirements, which result from its characteristic root structure. The adverse impact of soil factors can be mitigated to a certain extent by the use of mycorrhizal fungi. In this chapter, besides the cultivation conditions of V. corymbosum, the pro-health properties of fruits resulting from the presence of bioactive compounds such as polyphenolic compounds, flavonoids, especially anthocyanins, will be presented. Besides, factors, such as environmental conditions, degree of ripeness and variety, affect the content of bioactive substances.

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Comparative Phytochemical Profiles and Antioxidant Enzyme Activity Analyses of the Southern Highbush Blueberry (Vaccinium corymbosum) at Different Developmental Stages

Cited by 62 publications

References 44 publications

Analysis of Antioxidant Activity and Flavonoids Metabolites in Peel and Flesh of Red-Fleshed Apple Varieties

Analysis of Antioxidant Activity and Flavonoids Metabolites in Peel and Flesh of Red-Fleshed Apple Varieties

Phytochemical Constituents and Antioxidant Enzyme Activity Profiles of Different Barley (Hordeum Vulgare L.) Cultivars at Different Developmental Stages

Soil Preparation, Running Highbush Blueberry (Vaccinium corymbosum L.) Plantation and Biological Properties of Fruits

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