Comparative Plant Transcriptome Profiling of Arabidopsis thaliana Col-0 and Camelina sativa var.<i>Celine</i>Infested with Myzus persicae Aphids Acquiring Circulative and Noncirculative Viruses Reveals Virus- and Plant-Specific Alterations Relevant to Aphid Feeding Behavior and Transmission

Chesnais, Quentin; Golyaev, Victor; Velt, Amandine; Rustenholz, Camille; Brault, Véronique; Pooggin, Mikhail M.; Drucker, Martin

doi:10.1128/spectrum.00136-22

Cited by 14 publications

(23 citation statements)

References 97 publications

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“…The discrepancy in the results for g15329 expression was likely due its weak expression changes that in general are difficult to detect by RT-qPCR because of the exponential amplification kinetics of this technique. We observed the same phenomenon in a previous validation experiment (Chesnais, Golyaev, et al, 2022).…”

Section: Quality Control and Validation Of Rna-seq Datasupporting

confidence: 86%

“…This might favor aphid mobility and survival and in turn virus dispersion. Genes involved in stress responses and saliva functions were mostly downregulated (except the saliva protein ACE1 contributing to lifespan), indicating that viral infection facilitates aphid infestation of the host plants, for example by dampening anti-herbivore plant defenses as observed in our previous study (Chesnais, Golyaev, et al, 2022).…”

Section: Common Deregulated Genes In Aphids Feeding On Camv-and Tuyv-...supporting

confidence: 66%

“…In the case of mock-inoculated Camelina, two of the three replicates clustered together and were well separated from the data for virus-infected Camelina, while the third replicate clustered with the aphid data from infected plants and was therefore excluded from further analysis (Figure 1b). The transcriptomes of the plants used here for aphid infestation were analyzed in another study (Chesnais, Golyaev, et al, 2022). There the three replicates from mock-infected Camelina clustered closely together in principal component analysis.…”

Section: Quality Control and Validation Of Rna-seq Datamentioning

confidence: 99%

“…stress-related genes (Table 1). Previously, we have demonstrated that plant infection with CaMV and TuYV alters primary and secondary metabolism in Arabidopsis and Camelina strongly (Chesnais, Golyaev, et al, 2022). Consequently, aphids could also be stressed on the infected plants because of these important alterations of the plants.…”

Section: Common Deregulated Genes In Aphids Feeding On Camv-and Tuyv-...mentioning

confidence: 99%

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Transcriptome responses of the aphid vector Myzus persicae are shaped by identities of the host plant and the virus

Chesnais¹,

Golyaev²,

Velt³

et al. 2022

Peer Community Journal

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Background: Numerous studies have documented modifications in vector orientation behavior, settling and feeding behavior, and/or fecundity and survival due to virus infection in host plants. These alterations are often expected to enhance virus transmission, which has led to the hypothesis that such effects are vector manipulations by the virus. However, until now, the gene expression changes correlating with these effects and indicative of modified vector pathways and mechanisms are mostly unknown. Results: Transcriptome profiling of Myzus persicae aphids feeding on turnip yellows virus (TuYV) and cauliflower mosaic virus (CaMV) infected Arabidopsis thaliana and Camelina sativa revealed a substantial proportion of commonly deregulated genes, amongst them many with general functions in plant-virus-aphid interactions. We identified also aphid genes specifically deregulated by CaMV or TuYV infection, which might be related to the viral transmission mode. Furthermore, we observed strong host-specific differences in the gene expression patterns with plant virus infection causing more deregulations of aphid genes on A. thaliana than on C. sativa, likely related to the differences in susceptibility of the plant hosts to these viruses. Finally, stress-related aphid genes were downregulated in M. persicae on both infected plants, regardless of the virus. Conclusions: TuYV, relying on the circulative persistent mode of transmission, tended to affect developmental genes. This could increase the proportion of alate aphids, but also affect their locomotion, neuronal activity, and lifespan. CaMV, using the non-circulative nonpersistent mode of transmission, had a strong impact on feeding-related genes and in particular those related to salivary proteins. In general, these transcriptome alterations targeted pathways that seem to be particularly adapted to the transmission mode of the corresponding virus and could be evidence of vector manipulation by the virus.

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Section: Quality Control and Validation Of Rna-seq Datasupporting

confidence: 86%

Section: Common Deregulated Genes In Aphids Feeding On Camv-and Tuyv-...supporting

confidence: 66%

Section: Quality Control and Validation Of Rna-seq Datamentioning

confidence: 99%

Section: Common Deregulated Genes In Aphids Feeding On Camv-and Tuyv-...mentioning

confidence: 99%

See 2 more Smart Citations

Transcriptome responses of the aphid vector Myzus persicae are shaped by identities of the host plant and the virus

Chesnais¹,

Golyaev²,

Velt³

et al. 2022

Peer Community Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…The copyright holder for this preprint this version posted December 10, 2022. ; https://doi.org/10.1101/2022.12.09.519780 doi: bioRxiv preprint (E) Log2foldchange of ABA responsive genes ("upregulated after ABA treatment", n=651, (Hoth et al, 2002)) in CaMV infected compared to mock plants in the transcriptome dataset of (Chesnais et al, 2022) (F) Log2foldchange of ABA responsive genes ("downregulated after ABA treatment", n=680, (Hoth et al, 2002)) in CaMV infected compared to mock plants in the transcriptome dataset of (Chesnais et al, 2022) (G) relative transcript accumulation of ABA responsive genes (from category "up") in Col-0 plants at 21 dpi mock or CaMV infection normalized to PP2a. n=3 Accessions within the circles are written below the graph and named by their accession identifier.…”

Section: Discussionmentioning

confidence: 99%

Cauliflower mosaic virusdisease spectrum uncovers novel susceptibility factor NCED9 inArabidopsis thaliana

Hoffmann

Shukla

López‐González

et al. 2022

Preprint

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Viruses are intimately linked with their hosts and especially dependent on gene-for-gene interactions to establish successful infections. The genotype of their hosts thus has a strong influence on the outcome virus disease. On the host side, defence mechanisms like tolerance and resistance can occur within the same species leading to differing virus accumulation in relation to symptomology and plant fitness. The identification of novel resistance genes and susceptibility factors against viruses is an important part in understanding viral pathogenesis and securing food production. The model plant Arabidopsis thaliana displays a wide symptom spectrum in response to RNA virus infections and unbiased genome-wide association studies have proven a powerful tool to identify novel disease-genes. In this study we infected natural accessions of Arabidopsis thaliana with the pararetrovirus Cauliflower mosaic virus to study the phenotypic variations between accessions and their correlation with virus accumulation. Through genome-wide association mapping of viral accumulation differences, we identified several susceptibility factors for CaMV, the strongest of which was the abscisic acid synthesis gene NCED9. Further experiments confirmed the importance of abscisic acid homeostasis and its disruption for CaMV disease.

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Facilitation of Sclerotinia sclerotiorum infestation by aphid feeding behaviour is not affected by aphid resistance in oilseed rape

Hao,

Feng,

Sheng

et al. 2024

Heliyon

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Comparative Plant Transcriptome Profiling of Arabidopsis thaliana Col-0 and Camelina sativa var.CelineInfested with Myzus persicae Aphids Acquiring Circulative and Noncirculative Viruses Reveals Virus- and Plant-Specific Alterations Relevant to Aphid Feeding Behavior and Transmission

Cited by 14 publications

References 97 publications

Transcriptome responses of the aphid vector Myzus persicae are shaped by identities of the host plant and the virus

Transcriptome responses of the aphid vector Myzus persicae are shaped by identities of the host plant and the virus

Cauliflower mosaic virusdisease spectrum uncovers novel susceptibility factor NCED9 inArabidopsis thaliana

Facilitation of Sclerotinia sclerotiorum infestation by aphid feeding behaviour is not affected by aphid resistance in oilseed rape

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