Comparative pollen and foliar micromorphological studies using light microscopy and scanning electron microscopy of some selected species of Lamiaceae from Alpine Zone of Deosai Plateau, Western Himalayas

Abstract

The study was conducted to highlight a detailed account of morphology of pollen chosen species of Lamiaceae through scanning electron microscopy, and the anatomical characteristics of leaf epidermis of seven species using simple light microscopy.In results, Anisomeles indica and Otostegia aucheri belong to subfamily Lamioideae because it has tricolpate pollen while the rest eight species belong to subfamily Nepetoideae (hexacolpate pollen). The exine sculpturing of pollen of studied species was found to be reticulate. In the family Lamiaceae, four kinds of stomata were found anomocytic, anisocytic, diacytic, and actinocytic, respectively. The cell wall patterns of epidermal cells were irregular or polygonal with straight or undulate walls. It was noted that the variety of the epidermal trichomes seems of taxonomically important for the identification of species of Lamiaceae. Both nonglandular and glandular trichomes were analyzed. The nonglandular trichomes were characterized with long, thin, and pointed apical unicellular cells. The nonglandular trichomes were A-shaped in Thymus linearis. In Perovskia abrotanoides, stellate glandular trichomes were observed whereas in A. indica and Mentha royleana both glandular and nonglandular trichomes were found. In A. indica, the nonglandular trichomes were sessile and peltate in M. royleana. For the first time in this study, pollen and foliar micromorphological features of selected species of this area are carried out. These taxonomic characters were found to be important in discrimination of species from each other.In future, the detailed study with comprehensive morphology coupled with other important characters is required for delimitation of taxa at various levels. K E Y W O R D Slight microscopy, microscopic anatomy, pollen microscopy, scanning electron microscopy, surface analysis

Scanning electron imaging of mellitophilous and allergenic pollen grain of arid and northern irrigated agroecological zones of Pakistan

“…Harley et al (2004) further classified Nepetoideae into three tribes, Ocimeae, Mentheae, and Elsholzeae. Generally, they have radially symmetrical, isopolar (Bano et al, 2020) pollen ranging from oblate to spheroidal pollen having 3-6 colpi. In the present study, pollen are observed to belong to Hissopus genus (Figure 35a,b with six colpi, equidistantly arranged, size of the pollen range between 22 and 24 μm.…”

Changes in Foliar Functional Traits of S. pyrenaicus subsp. carpetanus under the Ongoing Climate Change: A Retrospective Survey

“…Furthermore, long-term anatomical adjustments in SD and SS ultimately target an improved stomatal sensitivity and faster adjustment of aperture in response to changing environments, thus having an effect on the cost-effectiveness of photosynthesis [18]. In turn, assessing modifications in stomatal patterning and structures in leaves from conserved specimens (a morphological trait well conserved in ex situ collections for prolonged periods) provides insight on the conditions and plant responses at the time of collection [19,20]. Additionally, given the strong link between stomata and water loss in plants, evaluating stomatal patterning modifications through time in collected individuals could also enable unveiling the response to impending warmer and drier growth seasons, particularly in water-limited environments such ass Mediterranean high-mountains [12,16,21,22].…”

Palyno‐morphological diversity of Asteraceous and Poaceous allergenic plant using microscopic techniques in lesser Himalaya‐Pakistan

“…Study of pollen grains and spores called "Palynology" (palynos and dust). Ultrastructural and morphological features of pollen grains provide a wealth characteristic to identify a specific plant taxon (Ahmad et al, 2018;Ahmed et al, 2019;Bano et al, 2012Bano et al, , 2020Simpson, 2019). To place taxa in correct taxonomic rank pollen morphological features are used by taxonomists for identification of flora (Khan et al, 2021).…”

“…Taxonomists employ a variety of tools linked to plant taxonomy to aid in the identification systematically, classification, and positioning of plants (Bano et al, 2012(Bano et al, , 2020. Morphology of pollen have paid crucial role in classification and identification of plants.…”