Comparative protein profile studies and in silicostructural/functional analysis of HMGR (ApHMGR)in Andrographispaniculata (Burm.f.) Wall. ex Nees

Bindu, Byreddi Bhavani Venkata; Srinath, Mote; Shailaja, Aayeti; Giri, Charu Chandra

doi:10.21276/ap.2017.6.1.5

Cited by 6 publications

(3 citation statements)

References 35 publications

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“…The terpenoid metabolic pathway is the most important pathway for the synthesis of special aroma substances in rose fragrance grapes [9]. Carotenoids, the product of the MEP metabolic pathway, contain multiple conjugated double bonds, which can form lycopene, capsicum red pigment, and other substances after cyclization to reveal the wide array of colors [10]. Studying the synthesis of terpenoids and their regulation mechanism is an urgent need to increase the content of terpenoids and achieve the improvement of fruit quality.…”

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confidence: 99%

Expressional diversity of grapevine 3-Hydroxy-3-methylglutaryl-CoA reductase (VvHMGR) in different grapes genotypes

Zheng

Guan

et al. 2021

BMC Plant Biol

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Background 3-Hydroxy-3-methylglutaryl-CoA reductase (HMGR) is a key enzyme in the mevalonate (MVA) pathway, which regulates the metabolism of terpenoids in the cytoplasm and determines the type and content of downstream terpenoid metabolites. Results Results showed that grapevine HMGR family has three members, such as VvHMGR1, VvHMGR2, and VvHMGR3. The expression of VvHMGRs in 'Kyoho' has tissue specificity, for example, VvHMGR1 keeps a higher expression, VvHMGR2 is the lowest, and VvHMGR3 gradually decreases as the fruit development. VvHMGR3 is closely related to CsHMGR1 and GmHMGR9 and has collinearity with CsHMGR2 and GmHMGR4. By the prediction of interaction protein, it can interact with HMG-CoA synthase, MVA kinase, FPP/GGPP synthase, diphosphate mevalonate decarboxylase, and participates in the synthesis and metabolism of terpenoids. VvHMGR3 have similar trends in expression with some of the genes of carotenoid biosynthesis and MEP pathways. VvHMGR3 responds to various environmental and phytohormone stimuli, especially salt stress and ultraviolet (UV) treatment. The expression level of VvHMGRs is diverse in grapes of different colors and aroma. VvHMGRs are significantly higher in yellow varieties than that in red varieties, whereas rose-scented varieties showed significantly higher expression than that of strawberry aroma. The expression level is highest in yellow rose-scented varieties, and the lowest in red strawberry scent varieties, especially ‘Summer Black’ and ‘Fujiminori’. Conclusion This study confirms the important role of VvHMGR3 in the process of grape fruit coloring and aroma formation, and provided a new idea to explain the loss of grape aroma and poor coloring during production. There may be an additive effect between color and aroma in the HMGR expression aspect.

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confidence: 99%

Expressional diversity of grapevine 3-Hydroxy-3-methylglutaryl-CoA reductase (VvHMGR) in different grapes genotypes

Zheng

Guan

et al. 2021

BMC Plant Biol

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“…These findings substantiated the rate limiting characteristics of DXS in the MEP pathway. Structural and functional information of rate limiting enzymes of these pathways is paramount in the study of biochemical properties for isolation of the protein, elucidating the 3D protein models for drug designing and protein interaction studies in the biosynthesis of secondary metabolites (Elizabeth et al, 2011;Singh et al, 2014;Bindu et al, 2017). The modification of metabolic flux via altering the DXS expression increases the scope for increased synthesis of diterpene lactone ultimately elevated andrographolide production (Pulido et al, 2012;Simpson et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Further, in our laboratory we have an inclusive research agenda involving studies on the distribution of A. paniculata and related species, haplotyping, selection and yield augmentation of bioactive compounds, using biotechnological and molecular approaches (Neeraja et al, 2015;Arolla et al, 2015;Parlapally et al, 2015;Zaheer and Giri, 2015;Neeraja et al, 2016;Bindu et al, 2017;Zaheer and Giri, 2017a;Zaheer and Giri, 2017b). In the present communication, we report the in silico investigation on structural, functional and evolutionary inter relationships of ApDXS and its interaction with MVA and MEP pathway proteins.…”

Section: Introductionmentioning

confidence: 99%

Characterization of 1-deoxy-D-xylulose 5-phosphate synthase (DXS) protein in Andrographis paniculata (Burm.f.) Wall. ex. Nees: A in silico appraisal

Srinath¹,

Shailaja²,

Bindu³

et al. 2017

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Andrographis paniculata (Burm.f.) Wall. ex Nees is as an important medicinal plant from centuries for treating infectious diseases in India and other countries. Active principles of this plant are diterpene lactones, specifically andrographolides and are synthesised via two independent biosynthetic pathways, i.e., mevalonic acid (MVA) and methyl-erythritol phosphate (MEP) pathway. Very meagre genomic and proteomic information is available about the genes and enzymes involved in these biosynthetic pathways. In this study, we have performed in silico characterization of a vital rate limiting enzyme 1-deoxy-D-xylulose 5phospahte synthase (DXS) of MEP pathway in A. paniculata. Structural and functional characterization of A. paniculata DXS (ApDXS) revealed its hydrophobic nature and a non trans-membrane protein was found to be present in chloroplast stroma. Predicted 3D structure with phyre2 tool had shown 85% of amino acids in the most favoured region as revealed by Ramachandran plot and was 96.32% structurally reliable. The phylogenetic analysis of ApDXS by MEGA7 revealed evolutionarily close relationship with Lamiaceae (Phlomis umbrosa/ Phlomoides umbrosa, Plectranthus barbatus, Lavandula angustifolia and Salvia miltiorrhiza) and Pedaliaceae (Sesamum indicum) families. Protein-protein interaction study revealed the interface of ApDXS with other MEP pathway proteins such as HDS, HDR, DXR and CDPMEK. Further, interaction was also evident with MVA pathway protein HMGS and downstream proteins, viz., GPS1, IPPI1 of diterpenoid pathway. The findings on the interactions of ApDXS with HMGS has given insight to the cross talk between MEP and MVA pathways. Prediction of bio-physico-chemical properties, secondary and tertiary structures will be of significance in protein purification processes. In addition, it will also be advantageous for drug designing applications with particular reference to manipulation of biosynthetic pathway, involving diterpene lactones in A. paniculata.

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Anti-cancer labdane diterpenoids from adventitious roots of Andrographis paniculata: augmentation of production prospect endowed with pathway gene expression

et al. 2018

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Andrographolide (AD) is the time-honoured pharmacologically active constituent of the traditionally renowned medicinal plant-Andrographis paniculata. Advancements in the target-oriented drug discovery process have further unravelled the immense therapeutic credibility of another unique molecule-neoandrographolide (NAD). The escalated market demand of these anti-cancer diterpenes is increasingly facing unrelenting hurdles of demand and supply disparity, attributable to their limited yield. Callus and adventitious root cultures were generated to explore their biosynthetic potentials which first time revealed NAD production along with AD. Optimization of the types and concentrations of auxins along with media form and cultivation time led to the successful tuning towards establishing adventitious roots as a superior production alternative for both AD/NAD. Supplementation of IBA to the NAA + Kn-containing MS medium boosted the overall growth and AD/NAD synthesis in the adventitious roots. Compared to control leaves, the adventitious root exhibited about 2.61- and 8.8-fold higher contents of AD and NAD, respectively. The qRT-PCR involving nine key pathway genes was studied, which revealed upregulation of GGPS1 and HMGR1/2 genes and downregulation of DXS1/2 and HDR1/2 genes in the adventitious root as compared to that in the control leaves. Such observations highlight that in vitro cultures can serve as efficient production alternatives for AD/NAD as the cytosolic genes (HMGR1/2 of MVA pathway) are competent enough to take over from the plastidial genes (DXS1/2 and HDR1/2 of MEP pathway), provided the accredited first branch-point regulatory gene (GGPS) expression and the culture requirements are optimally fulfilled.

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Comparative protein profile studies and in silicostructural/functional analysis of HMGR (ApHMGR)in Andrographispaniculata (Burm.f.) Wall. ex Nees

Cited by 6 publications

References 35 publications

Expressional diversity of grapevine 3-Hydroxy-3-methylglutaryl-CoA reductase (VvHMGR) in different grapes genotypes

Expressional diversity of grapevine 3-Hydroxy-3-methylglutaryl-CoA reductase (VvHMGR) in different grapes genotypes

Characterization of 1-deoxy-D-xylulose 5-phosphate synthase (DXS) protein in Andrographis paniculata (Burm.f.) Wall. ex. Nees: A in silico appraisal

Anti-cancer labdane diterpenoids from adventitious roots of Andrographis paniculata: augmentation of production prospect endowed with pathway gene expression

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