Comparative proteome analysis of amniotic fluids and placentas from patients with idiopathic polyhydramnios

Cen, Jiao; Lv, Liqin; Wei, Yiyun; Deng, Linjie; Huang, Le; Deng, Xiangyuan; Qin, Qinhong; Yan, Sun; Pang, Lihong

doi:10.1016/j.placenta.2019.10.010

Cited by 7 publications

(3 citation statements)

References 55 publications

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“…Moreover, the comparisons between proteomic profiles from diseased and healthy pregnancies may also elucidate the etiologies of obstetrical syndromes 5 . Indeed, several studies have examined the AF proteome in fetal genetic disorders, such as trisomy 21 (i.e., Down syndrome) 9 and Turner syndrome 10 , as well as in pregnancy complications that may include polyhydramnios 11 , intra-amniotic inflammation 12 , preterm prelabor rupture of the membranes 13 , preeclampsia 14 , and spontaneous preterm labor 15 . Consequently, several AF proteins have been proposed as biomarkers for perinatal complications 3 .…”

Section: Introductionmentioning

confidence: 99%

The amniotic fluid proteome changes with gestational age in normal pregnancy: a cross-sectional study

Bhatti

Romero

Gomez‐Lopez

et al. 2022

Sci Rep

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The cell-free transcriptome in amniotic fluid (AF) has been shown to be informative of physiologic and pathologic processes in pregnancy; however, the change in AF proteome with gestational age has mostly been studied by targeted approaches. The objective of this study was to describe the gestational age-dependent changes in the AF proteome during normal pregnancy by using an omics platform. The abundance of 1310 proteins was measured on a high-throughput aptamer-based proteomics platform in AF samples collected from women during midtrimester (16–24 weeks of gestation, n = 15) and at term without labor (37–42 weeks of gestation, n = 13). Only pregnancies without obstetrical complications were included in the study. Almost 25% (320) of AF proteins significantly changed in abundance between the midtrimester and term gestation. Of these, 154 (48.1%) proteins increased, and 166 (51.9%) decreased in abundance at term compared to midtrimester. Tissue-specific signatures of the trachea, salivary glands, brain regions, and immune system were increased while those of the gestational tissues (uterus, placenta, and ovary), cardiac myocytes, and fetal liver were decreased at term compared to midtrimester. The changes in AF protein abundance were correlated with those previously reported in the cell-free AF transcriptome. Intersecting gestational age-modulated AF proteins and their corresponding mRNAs previously reported in the maternal blood identified neutrophil-related protein/mRNA pairs that were modulated in the same direction. The first study to utilize an aptamer-based assay to profile the AF proteome modulation with gestational age, it reveals that almost one-quarter of the proteins are modulated as gestation advances, which is more than twice the fraction of altered plasma proteins (~ 10%). The results reported herein have implications for future studies focused on discovering biomarkers to predict, monitor, and diagnose obstetrical diseases.

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Section: Introductionmentioning

confidence: 99%

The amniotic fluid proteome changes with gestational age in normal pregnancy: a cross-sectional study

Bhatti

Romero

Gomez‐Lopez

et al. 2022

Sci Rep

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“…The results of this study showed that polyhydramnios (RR: 1.996, 95% CI: 1.322-3.014) was higher in pregnant women with high serum free β-hCG levels than that in pregnant women with normal levels of the hormone, consistent with previous studies that reported a correlation of second-trimester non-immune hydrops (including hydramnios) with elevated maternal hCG levels [18] as well as a correlation of placental chorioangioma with early severe polyhydramnios and elevated maternal serum hCG levels [19]. In China, researchers have used proteomic techniques to identify many biomarkers for pregnancy-related pathological conditions, such as β-hCG for Down's syndrome and AFP for trisomy 13,18 syndrome, which were almost derived from or secreted by fetal, maternal circulation, and placental tissues [20]. The cohort study of Tashfeen et al [21] showed that polyhydramnios could increase the risk of adverse perinatal outcomes, and polyhydramnios was positively correlated with maternal age, diabetes, fetal malformation and macrosomia.…”

Section: Discussionmentioning

confidence: 99%

Relationship between increased maternal serum free human chorionic gonadotropin levels in the second trimester and adverse pregnancy outcomes: a retrospective cohort study

Chen,

Dai,

et al. 2024

BMC Women's Health

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Background A retrospective cohort study was conducted to collect the data of pregnant women who received hospital delivery in Hangzhou Women's Hospital from January 2018 to December 2020, and who participated in the second trimester (15–20+6 weeks) of free beta human chorionic gonadotropin (free β-hCG). And the study was conducted to explore the relationship between maternal serum free β-hCG and adverse pregnancy outcomes (APO). Methods We retrospectively analyzed the clinical data of 1,978 women in the elevated maternal serum free β-hCG group (free β-hCG ≥ 2.50 multiples of the median, MoM) and 20,767 women in the normal group (0.25 MoM ≤ free β-hCG < 2.50 MoM) from a total of 22,745 singleton pregnancies, and modified Poisson regression analysis was used to calculate risk ratios (RRs) and 95% confidence intervals (CI) of the two groups. Results The gravidity and parity in the elevated free β-hCG group were lower, and the differences between the groups were statistically significant (all, P < 0.05). The risks of polyhydramnios, preeclampsia, and hyperlipidemia, were increased in women with elevated free β-hCG levels (RRs: 1.996, 95% CI: 1.322–3.014; 1.469, 95% CI: 1.130–1.911 and 1.257, 95% CI: 1.029–1.535, respectively, all P < 0.05), intrauterine growth restriction (IUGR) and female infants were also likely to happen (RRs = 1.641, 95% CI: 1.103–2.443 and 1.101, 95% CI: 1.011–1.198, both P < 0.05). Additionally, there was an association between elevated AFP and free β-hCG levels in second-trimester (RR = 1.211, 95% CI: 1.121–1.307, P < 0.001). Conclusions APOs, such as polyhydramnios, preeclampsia, and hyperlipidemia, were increased risks of elevated free β-hCG levels, IUGR and female infants were also likely to happen. Furthermore, there was an association between elevated AFP levels and elevated free β-hCG levels in second-trimester. We recommend prenatal monitoring according to the elevated maternal serum free β-hCG level and the occurrence of APO.

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“…Previous studies have reported proteomics analysis of the human amnion for the identification of clinically significant biomarkers of problematic pregnancies (Hopkinson, McIntosh, Shanmuganathan, Tighe, & Dua, ; Park et al, ). A recent study utilized proteome analysis of the human placenta and amniotic fluid to explore the protein expression pattern in patients with polyhydramnios (Cen et al, ). However, there has been no study on proteomics analysis of the human amnion in conditions of oligohydramnios.…”

Section: Introductionmentioning

confidence: 99%

Altered proteomics profile in the amnion of patients with oligohydramnios

Cheung

Brace

2020

Physiol Rep

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In pregnancy, idiopathic oligohydramnios is an obstetrical complication that compromises maternal health with poor perinatal outcome. Effective therapeutic treatment of this condition has been hampered by the unknown etiology and lack of understanding of cellular and molecular mechanisms that underlie idiopathic oligohydramnios. Amniotic fluid volume (AFV) is determined by intramembranous (IM) transport of amniotic fluid across the amnion and this pathway is regulated to maintain AFV within the normal range. To gain understanding of the causes of idiopathic oligohydramnios, we performed proteomics analysis of the human amnion to investigate the changes in protein expression profiles of cellular transport pathways and regulators in patients with oligohydramnios. Placental amnions from five patients with normal pregnancies and five patients with oligohydramnios were subjected to proteomics experiments followed by bioinformatics analysis. Using Ingenuity Pathway Analysis (IPA) software, five categories of biological functions and multiple canonical pathways within each category were revealed. The top differentially expressed proteins that participate in mediating these pathways were identified. The functional pathways activated include: (a) cellular assembly and organization, (b) cell signaling and energy metabolism, and (c) immunological, infectious, and inflammatory functions. Furthermore, the analysis identified the category of pathways that facilitate molecular endocytosis and vesicular uptake. Under oligohydramniotic conditions, the mediators of clathrin vesicle‐mediated uptake and transport as well as intracellular trafficking mediators were up‐regulated. These findings suggest that idiopathic oligohydramnios may be associated with alternations in cellular organization and immunological functions as well as increases in activity of vesicular transport pathways across the amnion.

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Comparative proteome analysis of amniotic fluids and placentas from patients with idiopathic polyhydramnios

Cited by 7 publications

References 55 publications

The amniotic fluid proteome changes with gestational age in normal pregnancy: a cross-sectional study

The amniotic fluid proteome changes with gestational age in normal pregnancy: a cross-sectional study

Relationship between increased maternal serum free human chorionic gonadotropin levels in the second trimester and adverse pregnancy outcomes: a retrospective cohort study

Altered proteomics profile in the amnion of patients with oligohydramnios

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