Comparative proteome analysis of drought-sensitive and drought-tolerant rapeseed roots and their hybrid F1 line under drought stress

Abstract

Rapeseed (Brassica napus L.), which is the third leading source of vegetable oil, is sensitive to drought stress during the early vegetative growth stage. To investigate the initial response of rapeseed to drought stress, changes in the protein expression profiles of drought-sensitive (RGS-003) and drought-tolerant lines (SLM-003), and their F1 hybrid, were analyzed using a proteomics approach. Seven-day-old rapeseed seedlings were treated with drought stress by restricting water for 7 days, and proteins were extracted from roots and separated by two-dimensional polyacrylamide gel electrophoresis. In the sensitive rapeseed line, 35 protein spots were differentially expressed under drought stress, and proteins related to metabolism, energy, disease/defense, and transport were decreased. In the tolerant line, 32 protein spots were differentially expressed under drought stress, and proteins involved in metabolism, disease/defense, and transport were increased, while energy-related proteins were decreased. Six protein spots in F1 hybrid were common among expressed proteins in the drought-sensitive and -tolerant lines. Notably, tubulin beta-2 and heat shock protein 70 were decreased in the drought-sensitive line and hybrid F1 plants, while jasmonate-inducible protein and 20S proteasome subunit PAF1 were increased in the F1 hybrids and drought-tolerant line. These results indicate that (1) V-type H(+) ATPase, plasma-membrane associated cation-binding protein, HSP 90, and elongation factor EF-2 have a role in the drought tolerance of rapeseed; (2) The decreased levels of heat shock protein 70 and tubulin beta-2 in the drought-sensitive and hybrid F1 lines might explain the reduced growth of these lines in drought conditions.

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

“…The amount of this enzyme significantly decreased in the sample treated with moderate osmotic stress. A similar effect was also reported in other proteomic (Yan et al 2005;Toorchi et al 2009;Mohammadi et al 2012b) and transcriptomic (Espartero et al 1994) studies. This synthetase is probably involved in the control of gene expression under osmotic stress conditions due to its ability to methylate nucleic acids and, therefore, may constitute an interesting target for genetic engineering of plant resistance to this stress (Mohammadi et al 2012b).…”

“…HSPs play a protective function towards other proteins during various abiotic stresses. HSPs are involved in such processes as protein-protein interactions, protein folding, secretion and transport as well as protection against protein degradation and aggregation (Timperio et al 2008;Mohammadi et al 2012b). The quantity of a small HSP (sHSP22) significantly increased in the sample treated with moderate osmotic stress.…”

Comparative Proteomic Analysis of Brassica napus in Response to Drought Stress

“…In fact, proteins related to ATP synthesis, photosynthesis/carbon fixation, glycolysis, and stress and defense were commonly reported as drought and/or salt-responsive proteins from many other organisms 85 . In this study, the levels of proteins related to metabolism were increased in drought- 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 20 stressed samples, such as malate dehydrogenase, triosephosphate isomerase, glutamine synthetase, ATP synthase, and triosephosphate isomerase 12,30,52,84,86,87 Table 4 in the Supporting Information). In contrast to the increases in protein abundance, the activities of APX, CAT, SOD, and GST decreased in response to drought stress at the 10 th and 14 th day ( Figure 2).…”

Barley Grain Yield and Protein Content Response to Deficit Irrigation and Sowing Dates in Semi-Arid Region

“…Reduction of plant height under deficit irrigation may be due to reduction in cell elongation as a result of water stress. Furthermore, plant growth might have been retarded with smaller stomatal conductance, reduction in photosynthesis rate, and ion toxicity (Ashraf and Mcneilly, 2004;Mohammadi et al, 2012). Lower plant height might be caused from the weather condition (cold weather) in time of sowing since the primary growth of a plant is affected by late a sowing date.…”