Comparative proteome analysis of Helicobacter pylori clinical strains by two-dimensional gel electrophoresis

Abstract

Abstract:Objective: To investigate the pathogenic properties of Helicobacter pylori by comparing the proteome map of H. pylori clinical strains. Methods: Two wild-type H. pylori strains, YN8 (isolated from biopsy tissue of a gastric cancer patient) and YN14 (isolated from biopsy tissue of a gastritis and duodenal ulcer patient), were used. Proteomic analysis, using a pH range of 3-10 and 5-8, was performed. The individual proteins were identified by quadrupole time-of-flight (Q-TOF) mass spectrometer and protein database search. Results: Variation in spot patterns directed towards differential protein expression levels was observed between the strains. The gel revealed prominent proteins with several protein "families". The comparison of protein expressions of the two strains reveals a high variability. Differentially present or absent spots were observed. Nine differentially expressed protein spots identified by Q-TOF included adenosine triphosphate (ATP)-binding protein, disulfide oxidoreductase B (DsbB)-like protein, N utilization substance A (NusA), ATP-dependent protease binding subunit/heat shock protein, hydantoin utilization protein A, seryl-tRNA synthetase, molybdenum ABC transporter ModD, and hypothetical proteins. Conclusions: This study suggests that H. pylori strains express/repress protein variation, not only in terms of the virulence proteins, but also in terms of physiological proteins, when they infect a human host. The difference of protein expression levels between H. pylori strains isolated from gastric cancer and gastritis may be the initiator of inflammation, and result in the different clinical presentation. In this preliminary study, we report seven differential proteins between strains, with molecule weights from approximately 10 kDa to approximately 40 kDa. Further studies are needed to investigate those proteins and their function associated with H. pylori colonization and adaptation to host environment stress.

Expression of three essential antioxidants of Helicobacter pylori in clinical isolates

“…In a previous study, we investigated the comparative proteome of clinical H. pylori strains by two-dimensional gel electrophoresis. We reported differential proteins of H. pylori strains isolated from gastritis and gastric cancer, including antioxidants (Zhang et al, 2011). In this study, we investigated the expression of three essential antioxidants of H. pylori in clinical isolates to show the relationship between the expression of these antioxidants and different gastric diseases.…”

Proteome variability amongHelicobacter pyloriisolates clustered according to genomic methylation

“…; Zhang et al . , ). Our study is not an exception, and therefore, to achieve our goal, we sought to minimize this issue by selecting for the analysis the 2DE maps [previously obtained in (Vitoriano et al .…”

“…strains within each cluster. Given the complexity and high costs of the technology, proteomic studies are, in general, limited in respect to sample size (Smith et al 2007;Franco et al 2009;Zhang et al 2009Zhang et al , 2011. Our study is not an exception, and therefore, to achieve our goal, we sought to minimize this issue by selecting for the analysis the 2DE maps [previously obtained in (Vitoriano et al 2011a)] of the group of 11 epidemiologically and genetically unlinked H. pylori strains used in the restricted dendrogram ( Fig.…”

Education, Prevention, and Treatment of Helicobacter pylori Infection by General Practitioners

“…For patients with positive screening results, general practitioners will conduct a comprehensive assessment based on the patient's symptoms, signs, and possible risk factors. When necessary, they will recommend more accurate diagnostic methods such as gastroscopy and biopsy [17] . However, due to the limited popularity of gastroscopy in primary care institutions, general practitioners often need to rely on their clinical experience and judgment, as well as collaboration with higher-level medical institutions, to provide optimal treatment recommendations for patients.…”