Comparative proteomic analysis of bacterial wilt susceptible and resistant tomato cultivars

Afroz, Amber; Khan, Muhammad Rashid; Ahsan, Nagib; Komatsu, Setsuko

doi:10.1016/j.peptides.2009.06.005

Cited by 44 publications

(32 citation statements)

References 52 publications

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“…One protein (spot 39) from the plants relating to the protein folding, namely mitochondrial chaperonin-60 was also highly expressed with the increasing years of monoculture. The chaperonins, a ubiquitous family of sequence-related molecular chaperones, are essential for protein folding under both normal and stressful conditions [50], [51]. However, 6-phosphogluconate dehydrogenase (spot 60), a protein related to the pentose phosphate pathway was down-regulated in the two-year monoculture soil.…”

Section: Discussionmentioning

confidence: 99%

Comparative Metaproteomic Analysis on Consecutively Rehmannia glutinosa-Monocultured Rhizosphere Soil

et al. 2011

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BackgroundThe consecutive monoculture for most of medicinal plants, such as Rehmannia glutinosa, results in a significant reduction in the yield and quality. There is an urgent need to study for the sustainable development of Chinese herbaceous medicine.Methodology/Principal FindingsComparative metaproteomics of rhizosphere soil was developed and used to analyze the underlying mechanism of the consecutive monoculture problems of R. glutinosa. The 2D-gel patterns of protein spots for the soil samples showed a strong matrix dependency. Among the spots, 103 spots with high resolution and repeatability were randomly selected and successfully identified by MALDI TOF-TOF MS for a rhizosphere soil metaproteomic profile analysis. These proteins originating from plants and microorganisms play important roles in nutrient cycles and energy flow in rhizospheric soil ecosystem. They function in protein, nucleotide and secondary metabolisms, signal transduction and resistance. Comparative metaproteomics analysis revealed 33 differentially expressed protein spots in rhizosphere soil in response to increasing years of monoculture. Among them, plant proteins related to carbon and nitrogen metabolism and stress response, were mostly up-regulated except a down-regulated protein (glutathione S-transferase) involving detoxification. The phenylalanine ammonia-lyase was believed to participate in the phenylpropanoid metabolism as shown with a considerable increase in total phenolic acid content with increasing years of monoculture. Microbial proteins related to protein metabolism and cell wall biosynthesis, were up-regulated except a down-regulated protein (geranylgeranyl pyrophosphate synthase) functioning in diterpenoid synthesis. The results suggest that the consecutive monoculture of R. glutinosa changes the soil microbial ecology due to the exudates accumulation, as a result, the nutrient cycles are affected, leading to the retardation of plant growth and development.Conclusions/SignificanceOur results demonstrated the interactions among plant, soil and microflora in the proteomic level are crucial for the productivity and quality of R. glutinosa in consecutive monoculture system.

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Section: Discussionmentioning

confidence: 99%

Comparative Metaproteomic Analysis on Consecutively Rehmannia glutinosa-Monocultured Rhizosphere Soil

et al. 2011

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“…Proteomics provides insight into protein localization, protein–protein interactions, enzymatic complexes or post-translational modifications that are essential to a better understanding of plant-pathogen interactions. Proteomic approaches have been used in recent years to further characterize plant interactions with viruses (Casado-Vela et al, 2006; Giribaldi et al, 2011; Li et al, 2011; Di Carli et al, 2012), bacteria (Jones et al, 2006; Afroz et al, 2009, 2013; Li et al, 2012), or fungi (Kim et al, 2004a; Geddes et al, 2008; Bhadauria et al, 2010; Mukherjee et al, 2010; Shah et al, 2012). The general picture of changes occurring in the plant-host proteome highlights common features among the broad range of interaction analyzed.…”

Section: Introductionmentioning

confidence: 99%

Uncovering plant-pathogen crosstalk through apoplastic proteomic studies

et al. 2014

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Plant pathogens have evolved by developing different strategies to infect their host, which in turn have elaborated immune responses to counter the pathogen invasion. The apoplast, including the cell wall and extracellular space outside the plasma membrane, is one of the first compartments where pathogen-host interaction occurs. The plant cell wall is composed of a complex network of polysaccharides polymers and glycoproteins and serves as a natural physical barrier against pathogen invasion. The apoplastic fluid, circulating through the cell wall and intercellular spaces, provides a means for delivering molecules and facilitating intercellular communications. Some plant-pathogen interactions lead to plant cell wall degradation allowing pathogens to penetrate into the cells. In turn, the plant immune system recognizes microbial- or damage-associated molecular patterns (MAMPs or DAMPs) and initiates a set of basal immune responses, including the strengthening of the plant cell wall. The establishment of defense requires the regulation of a wide variety of proteins that are involved at different levels, from receptor perception of the pathogen via signaling mechanisms to the strengthening of the cell wall or degradation of the pathogen itself. A fine regulation of apoplastic proteins is therefore essential for rapid and effective pathogen perception and for maintaining cell wall integrity. This review aims to provide insight into analyses using proteomic approaches of the apoplast to highlight the modulation of the apoplastic protein patterns during pathogen infection and to unravel the key players involved in plant-pathogen interaction.

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“…Recently, functional approaches, including proteomics, have greatly contributed to the molecular dissection of plantepathogen interactions, allowing to identify a number of defence-related candidate proteins and helping to clarify specific gene expression patterns (Metha et al, 2008). In tomato, proteomic studies have been performed to investigate response to bacteria (Afroz et al, 2009;Dahal et al, 2010;Savidor et al, 2012), virus (Casado-Vela et al, 2006) and fungi (Houtermann et al, 2007;Mazzeo et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Tomato susceptibility to Fusarium crown and root rot: Effect of grafting combination and proteomic analysis of tolerance expression in the rootstock

Vitale

Rocco

Arena

et al. 2014

Plant Physiology and Biochemistry

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a b s t r a c tGrafting can enhance the tolerance of vegetable crops to soilborne diseases. The aim of this study was to investigate whether different tomato scionerootstock combinations may affect the plant susceptibility to Fusarium oxysporum f. sp. radicis-lycopersici (FORL), the causal agent of crown and root rot. A proteomic approach was used to investigate whenever the protein repertoire of the rootstock can be modified by FORL infection, in relation to cultivar susceptibility/tolerance to the disease. To this purpose, plants of tomato hybrids with different vigor, "Costoluto Genovese" (less vigorous) and "Kadima" (more vigorous), were grafted onto "Cuore di Bue" and "Natalia" hybrids, sensitive and tolerant versus FORL infections, respectively. Disease symptoms, plant biomasses, and protein expression patterns were evaluated 45 days after FORL inoculation. The extent of vascular discoloration caused by FORL in tomato plants grafted on "Natalia" rootstock (0.12e0.37 cm) was significantly lower than that of plants grafted on sensitive "Cuore di Bue" (1.75e6.50 cm). FORL symptoms significantly differed between "Costoluto Genovese" and "Kadima" scions only when grafted on sensitive rootstock. Shoot FW of non-inoculated "Kadima"/"Cuore di Bue" combination was 35% lower than "Kadima"/"Natalia", whereas no difference was manifested in inoculated plants. Shoot FW of inoculated "Costoluto Genovese"/"Cuore di Bue" combination was decreased of 39%, whereas that of "Costoluto Genovese"/"Natalia" of 11%, compared to control plants. Proteomic results showed a higher representation of proteins associated with pathogen infection in the tolerant rootstock, compared to the sensitive one, meaning a direct involvement of plant defence mechanisms in the tomato response to the pathogen challenge.

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Comparative proteomic analysis of bacterial wilt susceptible and resistant tomato cultivars

Cited by 44 publications

References 52 publications

Comparative Metaproteomic Analysis on Consecutively Rehmannia glutinosa-Monocultured Rhizosphere Soil

Comparative Metaproteomic Analysis on Consecutively Rehmannia glutinosa-Monocultured Rhizosphere Soil

Uncovering plant-pathogen crosstalk through apoplastic proteomic studies

Tomato susceptibility to Fusarium crown and root rot: Effect of grafting combination and proteomic analysis of tolerance expression in the rootstock

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