Comparative proteomic analysis of key proteins during abscisic acid-hydrogen peroxide-induced adventitious rooting in cucumber (Cucumis sativus L.) under drought stress

Li, Changxia; Bian, Biting; Gong, Tingyu; Liao, Weibiao

doi:10.1016/j.jplph.2018.07.012

Cited by 30 publications

(20 citation statements)

References 51 publications

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“…In our study, auxin-induced adventitious root formation was completely inhibited by preventing O 2 − production using DPI (Fig 4). These results are supported by observations in cucumber seedling explants, in which ethylene-, ABA-or auxin-induced adventitious root formation was eliminated by DPI [3,18,59]. In addition, our findings that the amounts of O 2 accumulated mainly in the differentiating adventitious root primordia (Fig 6F), but almost undetected when adventitious root primordia were completely differentiated ( Fig 6G) suggest that ROS are essential for adventitious root primordia induction.…”

Section: Plos Onesupporting

confidence: 91%

Genome-wide identification and characterization of Respiratory Burst Oxidase Homolog genes in six Rosaceae species and an analysis of their effects on adventitious rooting in apple

Cheng

Che

et al. 2020

PLoS ONE

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Adventitious root formation is essential for plant propagation, development, and response to various stresses. Reactive oxygen species (ROS) are essential for adventitious root formation. However, information on Respiratory Burst Oxidase Homolog (RBOH), a key enzyme that catalyzes the production ROS, remains limited in woody plants. Here, a total of 44 RBOH genes were identified from six Rosaceae species (Malus domestica, Prunus avium, Prunus dulcis 'Texas', Rubus occidentalis, Fragaria vesca and Rosa chinensis), including ten from M. domestica. Their phylogenetic relationships, conserved motifs and gene structures were analyzed. Exogenous treatment with the RBOH protein inhibitor diphenyleneiodonium (DPI) completely inhibited adventitious root formation, whereas exogenous H 2 O 2 treatment enhanced adventitious root formation. In addition, we found that ROS accumulated during adventitious root primordium inducing process. The expression levels of MdRBOH-H, MdRBOH-J, MdRBOH-A, MdRBOH-E1 and MdRBOH-K increased more than twofold at days 3 or 9 after auxin treatment. In addition, cis-acting element analysis revealed that the MdRBOH-E1 promoter contained an auxin-responsive element and the MdRBOH-K promoter contained a meristem expression element. Based on the combined results from exogenous DPI and H 2 O 2 treatment, spatiotemporal expression profiling, and cis-element analysis, MdRBOH-E1 and MdRBOH-K appear to be candidates for the control of adventitious rooting in apple.

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Section: Plos Onesupporting

confidence: 91%

Genome-wide identification and characterization of Respiratory Burst Oxidase Homolog genes in six Rosaceae species and an analysis of their effects on adventitious rooting in apple

Cheng

Che

et al. 2020

PLoS ONE

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“…Water shortages along with hydrogen peroxide and respiratory burst were involved in defense response and carbohydrate transport. These were significantly found and have confirmed to be associated with abiotic stresses in many plants [67]. Existing reports confirm that abscisic acid is involved in the metabolism of hydrogen peroxide (H 2 O 2 ) and calcium-dependent protein kinase (AtCPK1) to improve the drought resistance of plants [67,68].…”

Section: Discussionmentioning

confidence: 87%

“…These were significantly found and have confirmed to be associated with abiotic stresses in many plants [67]. Existing reports confirm that abscisic acid is involved in the metabolism of hydrogen peroxide (H 2 O 2 ) and calcium-dependent protein kinase (AtCPK1) to improve the drought resistance of plants [67,68]. Drought responses of carbohydrate metabolism may be important for plant acclimation to stress-this was later verified in Oryza sativa [53].…”

Section: Discussionmentioning

confidence: 87%

Identification of Exogenous Nitric Oxide-Responsive miRNAs from Alfalfa (Medicago sativa L.) under Drought Stress by High-Throughput Sequencing

Zhao

Wei

et al. 2019

Genes

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Alfalfa (Medicago sativa L.) is a high quality leguminous forage. Drought stress is one of the main factors that restrict the development of the alfalfa industry. High-throughput sequencing was used to analyze the microRNA (miRNA) profiles of alfalfa plants treated with CK (normal water), PEG (polyethylene glycol-6000; drought stress), and PEG + SNP (sodium nitroprusside; nitric oxide (NO) sprayed externally under drought stress). We identified 90 known miRNAs belonging to 46 families and predicted 177 new miRNAs. Real-time quantitative fluorescent PCR (qRT-PCR) was used to validate high-throughput expression analysis data. A total of 32 (14 known miRNAs and 18 new miRNAs) and 55 (24 known miRNAs and 31 new miRNAs) differentially expressed miRNAs were identified in PEG and PEG + SNP samples. This suggested that exogenous NO can induce more new miRNAs. The differentially expressed miRNA maturation sequences in the two treatment groups were targeted by 86 and 157 potential target genes, separately. The function of target genes was annotated by gene ontology (GO) enrichment and kyoto encyclopedia of genes and genomes (KEGG) analysis. The expression profiles of nine selected miRNAs and their target genes verified that their expression patterns were opposite. This study has documented that analysis of miRNA under PEG and PEG + SNP conditions provides important insights into the improvement of drought resistance of alfalfa by exogenous NO at the molecular level. This has important scientific value and practical significance for the improvement of plant drought resistance by exogenous NO.

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“…This promising region contains 46 important genes, and four of these genes were found with important functions namely SOUL heme-binding family protein (Araip.X9V0W), RING-H2 finger protein (Araip.YHU92), far1-related sequence 6-like (Araip.LKN71), ascorbate oxidase (Araip.K7Y13) and heavy metal transport/detoxification superfamily protein (Araip.2F1GS). Sequencing data suggested synonymous SNPs among genes encoding RING-H2 finger protein, far1-related sequence 6-like and SOUL heme-binding family protein, which are key regulator of developmental processes (Bu et al, 2009;Ko et al, 2006;Li et al, 2018). Likewise, a 0.74-Mb SNP-enriched genomic region was identified on the pseudomolecule A09 of A-subgenome, containing a nonsynonymous SNP on an ABA biosynthetic gene zeaxanthin epoxidase (ZEP, Aradu.D94AQ).…”

Section: Discussionmentioning

confidence: 99%

Whole‐genome resequencing‐based QTL‐seq identified candidate genes and molecular markers for fresh seed dormancy in groundnut

Kumar

Janila

Vishwakarma

et al. 2019

Plant Biotechnology Journal

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Summary The subspecies fastigiata of cultivated groundnut lost fresh seed dormancy (FSD) during domestication and human‐made selection. Groundnut varieties lacking FSD experience precocious seed germination during harvest imposing severe losses. Development of easy‐to‐use genetic markers enables early‐generation selection in different molecular breeding approaches. In this context, one recombinant inbred lines (RIL) population (ICGV 00350 × ICGV 97045) segregating for FSD was used for deploying QTL‐seq approach for identification of key genomic regions and candidate genes. Whole‐genome sequencing (WGS) data (87.93 Gbp) were generated and analysed for the dormant parent (ICGV 97045) and two DNA pools (dormant and nondormant). After analysis of resequenced data from the pooled samples with dormant parent (reference genome), we calculated delta‐SNP index and identified a total of 10,759 genomewide high‐confidence SNPs. Two candidate genomic regions spanning 2.4 Mb and 0.74 Mb on the B05 and A09 pseudomolecules, respectively, were identified controlling FSD. Two candidate genes—RING‐H2 finger protein and zeaxanthin epoxidase—were identified in these two regions, which significantly express during seed development and control abscisic acid (ABA) accumulation. QTL‐seq study presented here laid out development of a marker, GMFSD1, which was validated on a diverse panel and could be used in molecular breeding to improve dormancy in groundnut.

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Comparative proteomic analysis of key proteins during abscisic acid-hydrogen peroxide-induced adventitious rooting in cucumber (Cucumis sativus L.) under drought stress

Cited by 30 publications

References 51 publications

Genome-wide identification and characterization of Respiratory Burst Oxidase Homolog genes in six Rosaceae species and an analysis of their effects on adventitious rooting in apple

Genome-wide identification and characterization of Respiratory Burst Oxidase Homolog genes in six Rosaceae species and an analysis of their effects on adventitious rooting in apple

Identification of Exogenous Nitric Oxide-Responsive miRNAs from Alfalfa (Medicago sativa L.) under Drought Stress by High-Throughput Sequencing

Whole‐genome resequencing‐based QTL‐seq identified candidate genes and molecular markers for fresh seed dormancy in groundnut

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