Comparative proteomic analysis of liver antioxidant mechanisms in Megalobrama amblycephala stimulated with dietary emodin

Abstract

Oxidative stress is a toxicological endpoint that correlates with the nutrition status of fish through cellular damage, inflammation, and apoptosis. In order to understand the antioxidant mechanism induced by dietary emodin in Megalobrama amblycephala liver, a comparative proteomic analysis was performed to investigate the proteome alteration under emodin administration. 27 altered protein spots were separated under 30 mg kg−1 emodin stimulation based on 2-DE, and were all successfully identified using MALDI-TOF/TOF, representing 17 unique proteins. These proteins were functionally classified into antioxidant, metabolism, cytoskeleton, chaperone, signal transduction and cofactor groups. Network interaction and Gene Ontology annotation indicated 10 unique proteins were closely related to antioxidation and directly regulated by each other. Compared with the control group, administration of 30 mg kg−1 emodin significantly increased the antioxidant-related mRNA expressions of GPx1, GSTm and HSP70, but decreased the mRNA expressions of GAPDH and Sord, which was consistent with the protein expression. Nevertheless, Pgk1 and Aldh8a1 were up- and down-regulated, and ALDOB was down- and up-regulated at the mRNA and protein levels, respectively. These results revealed that the altered proteins enhanced antioxidation via complex regulatory mechanisms, and 30 mg kg−1 emodin was a suitable immunostimulant for M. amblycephala.

Dose and time effect of CdTe quantum dots on antioxidant capacities of the liver and kidneys in mice

“…23,24 The liver and kidneys are the most important organs for obtaining an overall assessment of the immune response and antioxidant activity. 25 We also studied QD-mediated toxicity across different time points and explored the roles of free Cd ions (Cd 2+ ) and ⋅OH in the liver and kidney damage. Our results showed that QD-induced histopathological changes were time dependent with elevated Cd 2+ and ⋅OH and could recover after a period of time.…”

Royal jelly enhanced the antioxidant activities and modulated the gut microbiota in healthy mice

“…As the most important organs for assessing overall antioxidation levels (Song et al, 2017), the antioxidation in both the liver and kidneys was increased by RJ. Numerous reports have demonstrated that RJ affects antioxidation in disease models (El-Hanoun et al, 2014).…”

Effects of emodin on inflammatory bowel disease-related osteoporosis

“…Previous studies have shown that natural compounds derived from herbs are available for the treatment of osteoporosis by promoting bone formation or inhibiting bone resorption [6,8,12]. Emodin is a natural compound and has shown great potential in anti-inflammatory [7], anti-cancer [18], anti-hyperglycemic [19] and anti-oxidant activities [11]. Several studies also reported the therapeutic effect of emodin on bone loss.…”

“…Natural compounds with anti-inflammatory effects may be promising alternative agents for the treatment of IBD-related bone loss. Emodin, a type of anthraquinone compound, derived from the roots and bark of numerous plants of the genus Rhamnus, has potential pharmacological effects, such as anti-cancer and anti-inflammatory activities [7,11]. A few studies have also indicated that emodin may have potential for the treatment of osteoporosis by inhibiting bone resorption or promoting bone formation [12,13].…”