Comparative proteomic analysis of NaCl stress-responsive proteins in Arabidopsis roots

Abstract

NaCl stress is a major abiotic stress limiting the productivity and the geographical distribution of many plant species. Roots are the primary site of salinity perception. To understand better NaCl stress responses in Arabidopsis roots, a comparative proteomic analysis of roots that had been exposed to 150 mM NaCl for either 6 h or 48 h was conducted. Changes in the abundance of protein species within roots were examined using two-dimensional electrophoresis. Among the >1000 protein spots reproducibly detected on each gel, the abundance of 112 protein spots decreased and 103 increased, at one or both time points, in response to NaCl treatment. Through liquid-chromatography-tandem mass spectrometry, identity was assigned to 86 of the differentially abundant spots. The proteins identified included many previously characterized stress-responsive proteins and others related to processes including scavenging for reactive oxygen species; signal transduction; translation, cell wall biosynthesis, protein translation, processing and degradation; and metabolism of energy, amino acids, and hormones. At the resolution of individual genes and proteins, poor statistical correlation (6 h, r= -0.13; 48 h, r=0.11) of these protein expression data with previous microarray results was detected, supporting the concept that post-transcriptional regulation plays an important role in stress-responsive gene expression, and highlighting the need for combined transcriptomic and proteomic analyses.

Nuclear Accumulation of Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase in Cadmium-Stressed Arabidopsis Roots

“…Perhaps the oxidative stress conditions imposed by cadmium activate a signaling cascade that enhances GAPC1 expression to compensate its ongoing inactivation. Interestingly, similarly to our results with cadmium, salt stress has been shown to induce, in Arabidopsis root cells, the accumulation of GAPC1 and proteins involved in ROS scavenging (Jiang et al, 2007).…”

Changes in the root proteome of Triticosecale grains germinating under osmotic stress

“…Similar results were also obtained in the study on Arabidopsis roots (Jiang et al 2007). Actin is a component of the microfilaments which are involved in transport of organelles as well as in the regulation of cell division and elongation (Liu 2011).…”

“…The plants' acclimation is related to the changes in gene expression and composition of the proteome and metabolome (Kosová et al 2011;Swigonska and Weidner 2013). Proteome studies allow the examination of the actual products of gene expression and post-translational modifications of proteins in plants tissues (Jiang et al 2007). The influence of salt or osmotic stress on the alterations in proteome was examined in tobacco (Dani et al 2005) and rice leaves (Salekdeh et al 2002).…”

EMBRYONIC FLOWER1 and ULTRAPETALA1 Act Antagonistically on Arabidopsis Development and Stress Response

“…To elucidate the molecular mechanism of the EMF1-mediated salt response, we studied the expression levels of two salt-responsive genes, a gene belonging to the peroxidase superfamily, AT2G38380, and a NAC domain protein gene, AT1G52890 (Tran et al, 2004;Jiang et al, 2007), in wild-type, LFYasEMF1, ult1-3, and LFYasEMF1 ult1-3 seedlings. As expected, salt increased, albeit to varying degrees, the transcript levels of these two genes in all four types of seedlings ( Fig.…”