Comparative proteomic analysis of peripheral blood mononuclear cells from atopic dermatitis patients and healthy donors

Kim, Won Kon; Cho, Hyun Ju; Ryu, Su In; Hwang, Hyang Ran; Kim, Do-Hyung; Ryu, Hye Young; Chung, Jin Woong; Kim, Tae Yoon; Park, Byoung Chul; Bae, Kwang‐Hee; Ko, Yong Sang; Lee, Sang Chul

doi:10.5483/bmbrep.2008.41.8.597

Cited by 18 publications

(12 citation statements)

References 37 publications

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“…Note A proteomic analysis of peripheral blood leukocytes demonstrated significant downregulation of NAPA expression in patients with atopic dermatitis as compared to control subjects (Kim et al, 2008). This decrease was validated by Western blotting analysis and NAPA level was suggested as a possible biomarker for the disease.…”

Section: Atopic Dermatitismentioning

confidence: 81%

NAPA (N-ethylmaleimide-sensitive factor attachment protein, alpha)

Naydenov¹,

Ivanov²

2014

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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Section: Atopic Dermatitismentioning

confidence: 81%

NAPA (N-ethylmaleimide-sensitive factor attachment protein, alpha)

Naydenov¹,

Ivanov²

2014

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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“…In order to provide further insight into the possible PMBCs alterations in MDS patients additional to those suggested by the 2D electrophoresis data, western blot analysis was performed for the two of identified proteins: fermitin-3 and vinculin. Both the proteins were uniquely identified in the corresponding spots (without coidentified proteins) and they are both involved in particular in cell adhesion and integrin signaling [ 56 , 57 ]. While vinculin was identified in five different spots with both increasing and decreasing spot normalized volumes (which strongly indicates the presence of posttranslational modifications and the alterations of individual proteoforms), fermitin-3 was observed in one spot only.…”

Section: Resultsmentioning

confidence: 99%

Peripheral Blood Mononuclear Cell Proteome Changes in Patients with Myelodysplastic Syndrome

Pecankova

Májek

Čermák

et al. 2015

BioMed Research International

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Our aim was to search for proteome changes in peripheral blood mononuclear cells (PBMCs) of MDS patients with refractory cytopenia with multilineage dysplasia. PBMCs were isolated from a total of 12 blood samples using a Histopaque-1077 solution. The proteins were fractioned, separated by 2D SDS-PAGE (pI 4–7), and double-stained. The proteomes were compared and statistically processed with Progenesis SameSpots; then proteins were identified by nano-LC-MS/MS. Protein functional association and expression profiles were analyzed using the EnrichNet application and Progenesis SameSpots hierarchical clustering software, respectively. By comparing the cytosolic, membrane, and nuclear fractions of the two groups, 178 significantly (P < 0.05, ANOVA) differing spots were found, corresponding to 139 unique proteins. Data mining of the Reactome and KEGG databases using EnrichNet highlighted the possible involvement of the identified protein alterations in apoptosis, proteasome protein degradation, heat shock protein action, and signal transduction. Western blot analysis revealed underexpression of vinculin and advanced fragmentation of fermitin-3 in MDS patients. To the best of our knowledge, this is the first time that proteome changes have been identified in the mononuclear cells of MDS patients. Vinculin and fermitin-3, the proteins involved in cell adhesion and integrin signaling, have been shown to be dysregulated in MDS.

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“…First-strand complementary DNA (cDNA) was synthesized using 2 µg of total RNA as the template, 500 ng of random primers, and cDNA synthesis kit components (Promega) in a total volume of 25 µL in accordance with the manufacturer’s recommendations [30]; [31]. The targeted fragment of cDNA for each of the brown adipocyte differentiation-associated genes (Table 1) was amplified by PCR with 2 µL of the reverse transcription (RT) product, 10 pmol of each primer, and a PCR premix (Intron, Daejeon, Korea).…”

Section: Methodsmentioning

confidence: 99%

Dual-Specificity Phosphatase 10 Controls Brown Adipocyte Differentiation by Modulating the Phosphorylation of P38 Mitogen-Activated Protein Kinase

Choi

Kim

et al. 2013

PLoS ONE

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BackgroundBrown adipocytes play an important role in regulating the balance of energy, and as such, there is a strong correlation between obesity and the amount of brown adipose tissue. Although the molecular mechanism underlying white adipocyte differentiation has been well characterized, brown adipocyte differentiation has not been studied extensively. Here, we investigate the potential role of dual-specificity phosphatase 10 (DUSP10) in brown adipocyte differentiation using primary brown preadipocytes.Methods and ResultsThe expression of DUSP10 increased continuously after the brown adipocyte differentiation of mouse primary brown preadipocytes, whereas the phosphorylation of p38 was significantly upregulated at an early stage of differentiation followed by steep downregulation. The overexpression of DUSP10 induced a decrease in the level of p38 phosphorylation, resulting in lower lipid accumulation than that in cells overexpressing the inactive mutant DUSP10. The expression levels of several brown adipocyte markers such as PGC-1α, UCP1, and PRDM16 were also significantly reduced upon the ectopic expression of DUSP10. Furthermore, decreased mitochondrial DNA content was detected in cells expressing DUSP10. The results obtained upon treatment with the p38 inhibitor, SB203580, clearly indicated that the phosphorylation of p38 at an early stage is important in brown adipocyte differentiation. The effect of the p38 inhibitor was partially recovered by DUSP10 knockdown using RNAi.ConclusionsThese results suggest that p38 phosphorylation is controlled by DUSP10 expression. Furthermore, p38 phosphorylation at an early stage is critical in brown adipocyte differentiation. Thus, the regulation of DUSP10 activity affects the efficiency of brown adipogenesis. Consequently, DUSP10 can be used as a novel target protein for the regulation of obesity.

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Comparative proteomic analysis of peripheral blood mononuclear cells from atopic dermatitis patients and healthy donors

Abstract: Atopic dermatitis (AD) is a chronic inflammatory skin disease that induces changes in various inflammatory

Cited by 18 publications

References 37 publications

NAPA (N-ethylmaleimide-sensitive factor attachment protein, alpha)

NAPA (N-ethylmaleimide-sensitive factor attachment protein, alpha)

Peripheral Blood Mononuclear Cell Proteome Changes in Patients with Myelodysplastic Syndrome

Dual-Specificity Phosphatase 10 Controls Brown Adipocyte Differentiation by Modulating the Phosphorylation of P38 Mitogen-Activated Protein Kinase

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