Comparative proteomic analysis of seed embryo proteins associated with seed storability in rice (Oryza sativa L) during natural aging

Gao, Jinghui; Fu, Hua; Zhou, Xinqiao; Chen, Zhongjian; Luo, Yuanyuan; Cui, Baiyuan; Chen, Guanghui; Liu, Jun

doi:10.1016/j.plaphy.2016.02.026

Cited by 55 publications

(46 citation statements)

References 33 publications

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“…With lower storage temperature, the abundance of fewer proteins changed, and cryopreservation in liquid nitrogen influenced only three proteins. Gao et al (2016) showed that seeds from rice cultivars sensitive to storage also displayed more differentially abundant proteins, in agreement with the present results.…”

Section: Discussionsupporting

confidence: 93%

“…Proteomic data has shown that reduction of seed longevity is often associated with oxidation of cellular macromolecules such as nucleic acids, proteins, and lipids (Sano et al 2016). Gao et al (2016) indicated that redox regulation proteins, mainly glutathione-related proteins, and defence proteins, including DNA-damage-repair proteins, and a late embryogenesis abundant (LEA) protein might have positive influence on seed storability. Min et al (2017) also highlighted the positive role of antioxidant enzymes dehydroascorbate reductase (DHAR), ascorbate peroxidase (APX), and superoxide dismutase (SOD), as well as peroxiredoxin, for seed longevity (Yin et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Proteomic analysis of black poplar (Populus nigra L.) seed storability

Pawłowski

Klupczyńska

Leśniewska

et al. 2019

Annals of Forest Science

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& Key message Adequate storability of black poplar (Populus nigra L.) seeds at temperatures below 0°C was associated with preservation of proteins of energy and carbohydrate metabolism, protein turnover, and proteins maintaining longterm stability of dehydrated tissue. & Context Understanding seed storability is a key factor for effective seed preservation and conservation. Black poplar is an endangered tree species and its seed loses rapidly viability during storage. & Aims The aim of this study was to determine, and functionally characterise, the proteins associated with storability of black poplar seeds. & Methods Dried seeds (7.1% MC) were stored at 3°C, − 3°C, − 20°C, and − 196°C, for a period of 12 and 24 months. Proteins were extracted and separated according to their isoelectric point (pI) and mass using 2-dimensional electrophoresis. Proteins that varied in abundance for temperature and time of storage were identified by mass spectrometry. & Results A germination test showed that seeds remained viable at − 3, − 20, and − 196°C. Storage at 3°C caused loss of seed viability. This loss in seed vigour was related to the largest changes in protein abundance. As storage temperature decreased, a smaller number of proteins displayed changed abundance. & Conclusion Good storability of black poplar seeds under freezing conditions may be associated with the following: an inhibition of synthesis of energy and carbohydrate metabolism and protein turnover proteins, LEA proteins that maintain long-term stability of dehydrated tissue, GDSL esterases/lipases that inhibit hydrolysing activity, and by chaperonins that protect protein functionality. Storage conditions influence the preservation of protein function that governs seed viability.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of black poplar (Populus nigra L.) seed storability

Pawłowski

Klupczyńska

Leśniewska

et al. 2019

Annals of Forest Science

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“…miRNAs are involved in regulating seed vigor It is well known that the storage capacity of hybrid rice seeds is not as high as that of conventional rice seeds (Gao et al 2016). Prolonging the life span of hybrid rice seeds is still a demanding task for breeders, seed producers, and seed traders.…”

Section: Discussionmentioning

confidence: 99%

miR164c and miR168a regulate seed vigor in rice

Zhou

Wang

et al. 2019

JIPB

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MicroRNAs (miRNAs) are key regulators of gene expression in many important biological processes of plants. However, few miRNAs have been shown to regulate seed vigor. Here, we conducted microarray assays to analyze miRNA expression levels in seeds of the rice (Oryza sativa L.) cultivar ZR02. Results showed significant differences in the expression of 11 miRNAs between artificially aged and untreated control seeds. Among these, osa‐miR164c was transcriptionally upregulated, while osa‐miR168a was downregulated in artificially aged seeds; this was verified by quantitative real‐time PCR analysis. Under the same aging condition, osa‐miR164c overexpression in OE164c transgenic seeds and osa‐miR168a silencing in MIM168a transgenic seeds of the rice cultivar Kasalath led to lower germination rates, whereas osa‐miR164c silencing in MIM164c and osa‐miR168a overexpression in OE168a resulted in higher seed germination rates compared with wild‐type seeds. Meanwhile, changes in cytomembrane permeability of seeds and in the expression level of osa‐miR164c target genes (OsPM27 and OsPSK5) and osa‐miR168a target genes (OsAGO1 and OsPTR2) under aging conditions coincided with changes in seed vigor induced by osa‐miR164c and osa‐miR168a. Thus, genetic manipulation of miRNAs has important implications in the development of crop cultivars with high vigor and extended life span of seeds.

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“…Moreover, seed aging also leads to ecological problems due to shortages of genetic resources and soil seed bank systems dysfunction, e.g., loss of plant biodiversity, grassland degeneration, and the enhancement of desertification [1]. Thus, loss of seed vigor is a major challenge for food and ecological security under natural conditions [2]. As a result, it is necessary to better understand the possible methods and mechanisms of maintaining seed vigor.…”

Section: Introductionmentioning

confidence: 99%

Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds

et al. 2020

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Background: Loss of vigor caused by seed aging adversely affects agricultural production under natural conditions. However, priming is an economical and effective method for improving the vigor of aged seeds. The objective of this study was to test the effectiveness of exogenous ascorbic acid (ASC) and glutathione (GSH) priming in the repairing of aged oat (Avena sativa) seeds, and to test the hypothesis that structural and functional systems in mitochondria were involved in this process. Results: Oat seeds were artificially aged for 20 days at 45°C, and were primed with solutions (1 mmol L − 1 ) of ASC, GSH, or ASC + GSH at 20°C for 0.5 h before or after their aging. Seed germination, antioxidant enzymes in the ASC-GSH cycle, cytochrome c oxidase (COX) and mitochondrial malate dehydrogenase (MDH) activities, and the mitochondrial ultrastructures of the embryonic root cells were markedly improved in aged oat seeds through post-priming with ASC, GSH, or ASC + GSH, while their malondialdehyde and H 2 O 2 contents decreased significantly (P < 0.05). Conclusion: Our results suggested that priming with ASC, GSH, or ASC + GSH after aging could effectively alleviate aging damage in oat seeds, and that the role of ASC was more effective than GSH, but positive effects of post-priming with ASC and GSH were not superior to post-priming with ASC in repairing aging damage of aged oat seeds. However, pre-priming with ASC, GSH, or ASC + GSH was not effective in oat seeds, suggesting that pre-priming with ASC, GSH, or ASC + GSH could not inhibit the occurrence of aging damage in oat seeds.

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Comparative proteomic analysis of seed embryo proteins associated with seed storability in rice (Oryza sativa L) during natural aging

Cited by 55 publications

References 33 publications

Proteomic analysis of black poplar (Populus nigra L.) seed storability

Proteomic analysis of black poplar (Populus nigra L.) seed storability

miR164c and miR168a regulate seed vigor in rice

Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds

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