Comparative Proteomic Analysis of Streptococcus suis Biofi lms and Planktonic Cells That Identifi ed Biofi lm Infection-Related Immunogenic Proteins

Abstract

is work aims at characterizing endoscope bio�lm-isolated �PAI� and reference strain P. aeruginosa �PA� adhesion, bio�lm formation and sensitivity to antibiotics. e recovery ability of the bio�lm-growing bacteria sub�ected to intermittent antibiotic pressure �cipro�oxacin �CIP� and gentamicin �GM��, as well as the development of resistance towards antibiotics and benzalkonium chloride �BC�, were also determined. e capacity of both strains to develop bio�lms was greatly impaired in the presence of CIP and GM. Sanitization was not complete allowing bio�lm recovery a�er the intermittent cycles of antibiotic pressure. e environmental pressure exerted by CIP and GM did not develop P. aeruginosa resistance to antibiotics nor cross-resistance towards BC. However, data highlighted that none of the antimicrobials led to complete bio�lm eradication, allowing the recovery of the remaining adhered population possibly due to the selection of persister cells. is feature may lead to bio�lm recalcitrance, reinforcement of bacterial attachment, and recolonization of other sites.

Evaluation of Some Common Disinfectants Against Some Gram-Negative Bacteria

“…10 Pseudomonas aeruginosa is an important opportunistic pathogen, and it is one of the major causes of hospital-acquired infections. 11 Considering the evaluation of exposing bacteria to the disinfectants for ten minutes, the aim was to increase the probability of disinfectant efficacy on bacteria by the benefit of time factor, through mixing the disinfectant with bacteria with a proportion 1:1 for ten minutes to give enough time to increase the disinfectant activity, but Lenol was excluded in this experiment based on first experiment which has proved that this disinfectant was already active on bacterial isolates used. The other disinfectants were used in this experiment.…”