Comparative proteomics analysis of Spodoptera frugiperda cells during Autographa californica multiple nucleopolyhedrovirus infection

Abstract

BackgroundIncreasing evidence sugggest that in addition of balculovirus controling insect host, host cells also responds to balculovirus infection. However, compared to existing knowledge on virus gene, host cell responses are relatively poorly understood.MethodsIn this study, Spodoptera frugiperda (Sf9) cells were infected with Autographa californica multiple nucleopolyhedrovirus (AcMNPV). The protein composition and protein changes of Spodoptera frugiperda (Sf9) cells of different infection stages were analysed by isobaric tag for relative and absolute quantification (iTRAQ) techniques.ResultsA total of 4004 Sf9 proteins were identified by iTRAQ and 413 proteins were found as more than 1.5-fold changes in abundance. The 413 proteins were categorised according to GO classification for insects and were categorised into: biological process, molecular function and cellular component.ConclusionsThe determination of the protein changes in infected Sf9 cells would help to better understanding of host cell responses and facilitate better design of this virus-host cell interaction in pest insect control and other related fields.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-015-0346-9) contains supplementary material, which is available to authorized users.

Proteomics as a tool for tapping potential of entomopathogens as microbial insecticides

“…The study reported that the increase in expression of energy metabolism‐related proteins lead to production of ATP which is coherent with the previous study (Carinhas et al, ; Yu et al, ). However, Yu et al () found no overexpression of proapoptotic signal as reported from Carinhas et al (). This is due to the difference of sampling methods of both studies.…”

“…It can be postulated that more‐resistant host perhaps is lacking of recognition sites on their membrane for NPVs pathogenesis. Another proteomics study used isobaric tag for relative and absolute quantitation (iTRAQ) method to elucidate from the same S. frugiperda cell line (Yu et al, ). The study reported that the increase in expression of energy metabolism‐related proteins lead to production of ATP which is coherent with the previous study (Carinhas et al, ; Yu et al, ).…”

“…Another proteomics study used isobaric tag for relative and absolute quantitation (iTRAQ) method to elucidate from the same S. frugiperda cell line (Yu et al, ). The study reported that the increase in expression of energy metabolism‐related proteins lead to production of ATP which is coherent with the previous study (Carinhas et al, ; Yu et al, ). However, Yu et al () found no overexpression of proapoptotic signal as reported from Carinhas et al ().…”

iTRAQ-based proteomic technology revealed protein perturbations in intestinal mucosa from manganese exposure in rat models

“…Mn is a cofactor in numerous enzymatic processes, so it is essential for brain biology and physiology. But excessive levels of Mn in two neurodegenerative syndromes (Huntington and Parkinson's disease) result in neuronal loss in the basal ganglia, 51,52 pathological changes in the striatum, 53 as well as having effects on mitochondrial dysfunction and disruption of cellular energy metabolism. 7,54 Specically, we found that four proteins were involved in Huntington's disease, Alzheimer's disease and Parkinson's disease: Gapdh, Atp5g1, OC690675, and Creb1.…”

Quantitative proteomics of Sf21 cells during Baculovirus infection reveals progressive host proteome changes and its regulation by viral miRNA

“…(2011) and Yu et al . (2015) have studied the differential proteome of S. frugiperda cells after baculovirus infection at 6 hpi and 12 hpi intervals and identified differential regulation of 648 and 413 proteins respectively 14 , 15 . Recently, Xing et al .…”