Comparative Quantitative Phosphoproteomic Analysis of the Chicken Egg during Incubation Based on Tandem Mass Tag Labeling

Abstract

Protein phosphorylation plays an important role in protein structure and function. To investigate the role of egg protein phosphorylation in chicken embryonic development, a comparative and quantitative phosphoproteomic analysis of fertilized chicken egg white and yolk was performed during incubation. Overall, 215 phosphosites mapped onto 205 phosphopeptides corresponding to 100 phosphoproteins were identified. Among these phosphoproteins, 123 phosphosites from 62 egg proteins were found significantly changed (p < 0.05) at day 12 during incubation. Furthermore, GO analysis suggested that these differentially phosphorylated proteins were associated with various molecular functions, primarily including binding, molecular function regulator, and transport activity. Such findings in this study improved our understanding of the protein molecular functions involved in chicken embryonic development from a protein phosphorylation perspective.

Phosphoproteomic analysis of duck egg white and insight into the biological functions of identified phosphoproteins

“…(2014) also had similar findings, where they reported that royal jelly proteins phosphorylation was potentially driven by the FAM20C‐like protein through the [S‐x‐E] motif. Likewise, another study from our lab also suggested that FAM20C might be responsible for the phosphorylation of eggshell phosphoproteins (Sun et al., 2019) through recognizing [S‐x‐E] motif (Mann & Mann, 2015). In addition, we noted a striking similarity between our current study and a previous study from our lab (Yang et al, 2019) that mainly the phosphoproteins were present in the extracellular region of DEW and CEW, respectively.…”

Phosphoproteomic analysis of duck egg yolk provides novel insights into its characteristics and biofunctions

“…Most iron in the yolk (90%) is bound to phosvitin, 28 whereas 90% of zinc is bound to lipovitellin, 29 which also binds copper 30 . VTGs were more highly phosphorylated in DEY than CEY (Geng F, 2021, unpublished data), and phosphorylation was reported to enhance the metal binding ability 15 . Thus it could be speculated that the highly phosphorylated VTG in DEY might play an important role in providing the desired trace element and phosphorus for the duck embryo to meet its nutrient requirement.…”

“…30 VTGs were more highly phosphorylated in DEY than CEY (Geng F, 2021, unpublished data), and phosphorylation was reported to enhance the metal binding ability. 15 Thus it could be speculated that the highly phosphorylated VTG in DEY might play an important role in providing the desired trace element and phosphorus for the duck embryo to meet its nutrient requirement. Trace mineral deficiencies lead to reduced growth rate, abnormal organ development, and embryo death in extreme cases; thus, this study will provide useful information for efficient enhancement in duck growth.…”

“…14 Our previous proteomic study revealed that phosphorylation also plays a significant regulatory role in chicken embryonic development. 15 Recently, we identified 36 phosphoproteins in duck egg white, which were mostly involved in the binding, transport activity, biological regulation, and metabolic processes. 16 Phosphorylation supposedly plays a potential role in enhancing the biological functions of duck egg white proteins, such as metabolic activity, antibacterial and antiviral activity, and antioxidant ability.…”

“…Moreover, phosphorylation was reported as a promising tool for improving the functional and nutritional properties of food proteins, including heat stability, emulsifying properties, foaming properties, and gelling properties 14 . Our previous proteomic study revealed that phosphorylation also plays a significant regulatory role in chicken embryonic development 15 . Recently, we identified 36 phosphoproteins in duck egg white, which were mostly involved in the binding, transport activity, biological regulation, and metabolic processes 16 .…”