Comparative RNA-Seq profiling of a resistant and susceptible peanut (Arachis hypogaea) genotypes in response to leaf rust infection caused by Puccinia arachidis

Genic microsatellite marker characterization and development in little millet (Panicum sumatrense) using transcriptome sequencing

“…As they originate from coding regions, eSSRs markers are preferred for studying plant species 11 . Genic SSRs are found within gene sequences and are conserved; hence they can be applied to define alleles associated with important agronomical traits 12 , 13 .…”

Comparative Transcriptome Analysis Identified Candidate Genes for Late Leaf Spot Resistance and Cause of Defoliation in Groundnut

“…Of the seven stages, four stages belong to disease development (1DPI, 2 DPI, 3DPI and 7 DPI) and three stages (at 21DPI, 35DPI and 50DPI) as symptom development. Recently, an attempt was made for transcriptome analysis discovered DEGs for rust caused by Cercospora arachidicola [ 28 ] and early leaf spot (ELS) caused by Puccinia arachidis [ 29 ] while no such study for LLS resistance. In the present study for LLS resistance, a total of 1484 million RNA sequencing reads were generated for 48 samples and mapped on A- and B-subgenome with average mapping percentage 77.5% and 79.8%, respectively.…”

“…Where, the Aradu.Z87JB showed upregulation (25.5-fold) more during disease development stage and more (56.7-fold) during symptom development stage in GPBD 4 and ICGV 13208 as compared with TAG 24. It is important to note that the disease resistant NBS-LRR genes were also reported upregulated in ELS resistant GPBD 4 (4.3-fold) when compared with susceptible JL 24 for ELS disease under C. arachidicola infection [ 28 ] in groundnut. Similarly, under infection of rust causing P. arachidis infection the NBR-LRR class showed upregulation (3.3-fold) in rust resistant GPBD 4 when compare with susceptible JL 24 [ 28 ] groundnut.…”

“…It is important to note that the disease resistant NBS-LRR genes were also reported upregulated in ELS resistant GPBD 4 (4.3-fold) when compared with susceptible JL 24 for ELS disease under C. arachidicola infection [ 28 ] in groundnut. Similarly, under infection of rust causing P. arachidis infection the NBR-LRR class showed upregulation (3.3-fold) in rust resistant GPBD 4 when compare with susceptible JL 24 [ 28 ] groundnut. Therefore, the disease resistance NBS-LRR class from QTL regions can be used for improving the late leaf spot resistance in important groundnut cultivars in addition to two other foliar fungal diseases, rust and ELS resistance.…”

“…Transcriptome analysis also gives an advantage to study the expression of genes in previously reported QTL region [ 27 ]. Recently, a comparative transcriptome analysis between JL24 (susceptible) and GPBD 4 (resistance) was performed to identify the differentially expressed genes (DEGs) for rust resistance under infection of Puccinia arachidis [ 28 ] and early leaf spot resistance under infection of Cercospora arachidicola [ 29 ]. An introgression line, ICGV 13208, used in the present study was developed by introgressing the resistance genomic region from GPBD 4 in TAG 24 [ 30 ].…”