Comparative Sensitivity of Green Algae to Herbicides Using Erlenmeyer Flask and Microplate Growth-Inhibition Assays

Pavlić, Želimira; Stjepanović, Barbara; Horvatić, Janja; Peršić, Vesna; Puntarić, Dinko; Čulig, Josip

doi:10.1007/s00128-006-1001-3

Cited by 32 publications

(17 citation statements)

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“…IPN at 0.5 M (103 g l −1 ) inhibited 68% (±3%) of algae growth (Fig. 4B), which is similar to what was reported by Pavlić et al [25] (70% inhibition at 100 g l −1 ). After the treatment in the laccase-ABTS system, more than 95% of this toxicity disappeared, showing the very low toxicity of the transformation product mixture compared to IPN.…”

Section: Toxicity Of Transformation Productssupporting

confidence: 89%

“…Long reaction times were used to assure complete reaction of the pollutants and stabilisation of the transformation products. These solutions were then diluted 200 times (with IPN) or 20 times (with SMX) in the algae growth medium prior to the toxicity tests to achieve concentrations of pollutants (0.5 and 7.5 M for IPN and SMX, respectively) in the algae medium that still allow them to grow [24,25].…”

Section: Ecotoxicity Testmentioning

confidence: 99%

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Sulfamethoxazole and isoproturon degradation and detoxification by a laccase-mediator system: Influence of treatment conditions and mechanistic aspects

Margot

Copin

Gunten

et al. 2015

Biochemical Engineering Journal

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a b s t r a c tThe potential of laccase-mediator systems (LMS) for the removal and detoxification of two wastewater micropollutants, the antibiotic sulfamethoxazole (SMX) and the herbicide isoproturon (IPN), was assessed. The influence of various parameters on micropollutant oxidation rates, such as pH, mediator, enzyme and pollutant concentrations, was investigated with three mediators: 2,2 -azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), syringaldehyde (SA) and acetosyringone (AS). Both pollutants were completely transformed within a few hours in presence of laccase and ABTS, as well as, for SMX, in presence of AS or SA. The three mediators were consumed during the reaction (no catalytic reactions observed), at a ratio mediator/pollutant between 1.1 and 16 mol/mol. Faster oxidation kinetics were observed at lower pH values, but also higher mediator/pollutant ratios were required. Several transformation products were formed, including cross-coupled products. Product mixtures were always less toxic to algae than untreated pollutants. Finally, a kinetic model that could explain the experimental observations was established. Based on the findings in this study LMS appears to be a promising option to treat concentrated and potentially toxic industrial effluents.

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Section: Toxicity Of Transformation Productssupporting

confidence: 89%

Section: Ecotoxicity Testmentioning

confidence: 99%

Sulfamethoxazole and isoproturon degradation and detoxification by a laccase-mediator system: Influence of treatment conditions and mechanistic aspects

Margot

Copin

Gunten

et al. 2015

Biochemical Engineering Journal

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“…As pointed out in the paper of Pavlić et al (2006) microplate algal assays for freshwater algae offer many advantages over standard Erlenmeyer flask tests. A similar procedure was developed for marine algae.…”

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confidence: 99%

The Effect of Ni2+, Co2+, Zn2+, Cd2+ and Hg2+ on the Growth Rate of Marine Diatom Phaeodactylum tricornutum Bohlin: Microplate Growth Inhibition Test

Horvatić

Peršić

2007

Bull Environ Contam Toxicol

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Algae are predominantly aquatic organisms that must be able to discriminate between essential and non-essential heavy metal ions (Perales-Vela et al. 2006). Many of the trace metals are essential but toxic at elevated concentrations. Cobalt (Co 2+ ), although an essential element (Lustigman et al. 1995), seems to have a direct effect on P680 (El-Sheekh et al. 2003). According to El-Sheekh et al. (2003) higher Co 2+ concentrations had an inhibitory effect on O 2 uptake by the two algal species Monoraphidium minutum and Nitzchia perminuta. Zinc (Zn 2+ ) is also an essential metal and at elevated concentrations can be toxic and cause algal cell death. Hirata et al. (2001) established that in marine green algae Dunaliella tertiolecta glutathione and phytochelatins can be involved in Zn detoxification. Mercury (Hg 2+ ), a toxic but non-essential element, is a metal with redox capacity that can enhance the pro-oxidant status by reducing the antioxidant glutathion pool (Okamoto et al. 2001). Cadmium (Cd 2+ ) has no known biological functions and is a highly toxic metal to aquatic organisms. Some previous studies demonstrated that Cd can be a substitute of Zn in marine phytoplankton (Price and Morel 1990). Nickel is one of the toxic metals found in industrial wastewaters (Lustigman et al. 1995). Nickel caused reduction in dry weight and at the same time highly stimulated biosynthesis of sulfur containing amino acids in N. perminuta probably due to some chelating mechanisms against heavy metal toxicity (Osman et al. 2004).As pointed out in the paper of Pavlić et al. (2006) microplate algal assays for freshwater algae offer many advantages over standard Erlenmeyer flask tests. A similar procedure was developed for marine algae. Lukavský and Simmer (2001) miniaturized the ISO 10253 bioassay with the marine diatom Phaeodactylum tricornutum by using immunological plates with wells of 0.25 and 2.5 ml as cultivation vessels. Diatoms occupy a variety of habitats and are often the most abundant photosynthetic organisms in marine waters. P. tricornutum is one of the most used algal species in the marine bioassays because of its easy cultivation, it is also a prospective alga in aquaculture as food for, e.g., crustacean Artemia (Kvíderová and Lukavský 2003). Therefore, in this study we have investigated the influence of different heavy metals (Ni 2+ , Co 2+ , Zn 2+ , Cd 2+ and Hg 2+ ) on the growth rate of marine phytoplankton diatom P. tricornutum Bohlin (Bacillariophyceae) by miniaturized bioassay method. Materials and MethodsThe toxicity tests were carried out with the unicellular marine diatom P. tricornutum Bohlin 1897 strain CCAP 1052/1A obtained by Culture Collection of Algae and Protozoa, UK. The alga was cultivated at the Department of Biology, J. J. Strossmayer University in Osijek in the f/2 medium at 20°C under continuous white light exposed to irradiance by PAR 120 lmol m -2 s -1 measured with flat sensor. The basic design of toxicity tests followed the International Standard ISO 10253 marine algal growth inhibition te...

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“…In this work, brine shrimp and green algae, as representative aquatic organisms, were selected in order to evaluate the ecotoxicity of [Ch][MA] ILs …”

Section: Methodsmentioning

confidence: 99%

The ecotoxicity and tribological properties of choline monocarboxylate ionic liquid lubricants

Fan

Zhang

et al. 2019

Lubrication Science

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In this work, a series choline monocarboxylate ionic liquids ([Ch][MA] ILs) were synthesized. The physicochemical properties and lubricating efficiency of the ILs on copper and aluminum discs were systematically investigated. At the same time, their ecotoxicity was explored on aquatic organisms. In comparison with 1‐butyl‐3‐methyl imidazolium tetrafluoroborate (L‐B104), which is a kind of traditional IL, [Ch][MA] ILs exhibited excellent lubricating properties and low toxicities, thus can be considered as environmentally friendly lubricants in tribology.

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Comparative Sensitivity of Green Algae to Herbicides Using Erlenmeyer Flask and Microplate Growth-Inhibition Assays

Cited by 32 publications

References 0 publications

Sulfamethoxazole and isoproturon degradation and detoxification by a laccase-mediator system: Influence of treatment conditions and mechanistic aspects

Sulfamethoxazole and isoproturon degradation and detoxification by a laccase-mediator system: Influence of treatment conditions and mechanistic aspects

The Effect of Ni2+, Co2+, Zn2+, Cd2+ and Hg2+ on the Growth Rate of Marine Diatom Phaeodactylum tricornutum Bohlin: Microplate Growth Inhibition Test

The ecotoxicity and tribological properties of choline monocarboxylate ionic liquid lubricants

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