Comparative sequence analysis of American, European and Asian isolates of viruses in the genus Coltivirus.

Abstract

In this study, the basis for the classification of virus isolates grouped within the genus Coltivirus, family Reoviridae, is discussed. Sequences of dsRNA segments from American (segments 9-12), European (segment 12) and Asian (segments 7-12) isolates were characterized and polythetic criteria were defined for their taxonomic classification. These criteria (including sequence analysis) permitted the different species to be distinguished and classified into two groups. In both groups, subgroups were defined according to the degree of homology between the genomic sequences. American and European isolates are classified within

Isolates of Liao Ning Virus from Wild-Caught Mosquitoes in the Xinjiang Province of China in 2005

“…The level of nt identity detected in Seg-10 between the 27 new isolates ranges from 94.3% to 100%, with 99.0% to 100%aa identity in VP10. These levels are consistent with isolates from a single serotype of LNV [14] or BAV [11]. Comparisons of Seg-10 from the 27 new isolates, with the prototype strain of LNV-1 (LNV-NE9712), identified nucleotide substitutions in a total of ninety positions within the sequenced region (nt: 21–864), 76.6% of which were 3rd position changes, 7.8% 2 nd position and 15.6% 1 st position.…”

“…Comparisons of Seg-10 from the 27 new isolates, with the prototype strain of LNV-1 (LNV-NE9712), identified nucleotide substitutions in a total of ninety positions within the sequenced region (nt: 21–864), 76.6% of which were 3rd position changes, 7.8% 2 nd position and 15.6% 1 st position. Overall, the prototype LNV-1 and the new isolates showed 91.9% to 93.1% nt, and 94.2% to 96.6% aa identity, which is still consistent with membership of the same virus serotype (members of the same LNV serotype show >80% amino acid identity in VP10 [14], while members of the same BAV serotype show >89% amino acid identity in VP9 [11]). …”

“…Banna virus and Kadipiro virus replicate in both insect cells and in mice, although their replication in mammalian cells is restricted to BSR cells [11], [12], [13]. Liao ning virus, replicates in insect cells, in a wide range of primary and transformed mammalian cells, and in mice, causing lethal haemorrhages after two successive injections [14].…”

Coltiviruses and Seadornaviruses in North America, Europe, and Asia

“…The genome contains ≈29,000 bp and segment size ranges from 675 bp to 4,350 bp ( 18 , 19 ). The genomic dsRNA of CTFV has an electropherotype (Figure 2A) similar to that of CTFV-Ca.…”

Development of a DNA microarray platform for the detection of viruses transmitted by small mammals and arthropods

“…Colorado tick fever virus reported in USA and Eaych virus in Europe and Asia (Attoui et al, 1998;Charrel et al, 2004). …”