Comparative Structural Characterization of Jujube Polysaccharides by Different Levels of Purification

Abstract

The objective of this paper was to analyze and contrast the features of the structure that jujube polysaccharides extracted and purified using various techniques, four fractions, namely hot water extraction and 0.3 M NaCl‐PBS purification (PZMP3‐1 and PZMP3‐2), alkaline extraction and 0.3 M NaCl purification (SAZMP4), alkaline extraction and 0.3 M‐PBS NaCl purification (PZJRP), were obtained by column chromatography (Sephacryl S‐300) and Fast Flow (DEAE‐Sepharose). Chemical testing, gas chromatography, scanning electron microscopy, nuclear magnetic resonance spectroscopy, methylation analysis, high‐performance gel permeation chromatography, and atomic force microscopy were used to examine and evaluate their properties. Different purification methods had certain effects on the structural characteristics of a series of jujube polysaccharides. The results could provide fundamental knowledge to select desirable extraction and purification technologies for the preparation of pure jujube polysaccharides.This article is protected by copyright. All rights reserved