Comparative Studies of Bacillus Cereus Strains Isolated From Various Foods and Food Poisoning Outbreaks

Raeυuori, M.; Kiutamo, Tuomo; Niskanen, Aimo

doi:10.1186/bf03548437

Cited by 11 publications

(1 citation statement)

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“…All B. cereus involved in emetic foodborne infections produce cereulide and form a very homogenous group with distinct phenotypic properties (Pirttijarvi et al, 1999;Raevuori et al, 1977). They presumably represent a clonal lineage (Pirttijarvi et al,.…”

Section: Emetic Foodborne Intoxicationmentioning

confidence: 99%

Opinion of the Scientific Panel on biological hazards (BIOHAZ) on Bacillus cereus and other Bacillus spp in foodstuffs

2005

EFS2

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SUMMARYBacillus cereus is the cause of two kinds of foodborne diseases, an emetic (vomiting) intoxication due to the ingestion of a toxin (cereulide) pre-formed in the food and a diarrhoeal infection due to the ingestion of bacterial cells/spores which produce enterotoxins in the small intestine. Other Bacillus spp., B. subtilis, B. licheniformis, B. pumilus have more rarely beeen identified as agents of foodborne diseases characterized by diarrhoea and/or vomiting. Emetic intoxication is caused by a very homogeneous group of strains of B. cereus identified by their ability to produce cereulide. In contrast, B. cereus strains able to cause diarrhoea are not easy to identify because the mechanisms leading to infection are complex and diverse. Very little is known on the virulence mechanisms of other Bacillus spp and therefore it is not possible to identify the strains able to cause foodborne poisoning.In most instances, foodborne diseases caused by B. cereus were associated with 5 log to 8 log cells/spores per g of the food vehicle. However, in some outbreaks, lower numbers in the food (3 -4 log per g) were reported. Foodborne poisoning caused by other Bacillus spp. has always been linked to high numbers of cells/spores in the food vehicle (equal or more to 6 log per g).Bacillus cereus is ubiquitous and low numbers of its spores, too low to cause foodborne poisoning, can be found in a wide range of foodstuffs. Spores can germinate and multiply in humid, low acid foods, from 4-5°C to 55°C. However, strains able to multiply below 7°C, and strains able to multiply above 45°C, are not the most common. Emetic B. cereus are presumably unable to grow and produce their toxin cereulide below 10°C, or in the absence of oxygen. Other Bacillus spp. involved in foodborne poisoning cases are also frequent causes of food spoilage. Almost all kind of foods have been implicated in B. cereus foodborne poisoning. However, a majority of reported outbreaks were linked to the consumption of heat treated foods and frequently occurred in restaurant and catering establishments. Failure in refrigeration was frequently suspected. Cooked dishes containing pasta or rice were the main, but not the only, foods implicated in emetic intoxications.The major control measures are to control temperature and to establish HACCP system. Only heat treatments used for canning of low acid foods will ensure a complete destruction of spores of B. cereus. The number of spores in other processed foods must be kept as low as possible by proper cleaning and disinfection of equipments. Rapid cooling is necessary to prevent germination and growth of B. cereus spores. Low pH (below 4.5), reduction in a w (below 0. Community legislation on food hygiene is currently under revision. In this framework a revision of the microbiological criteria in Community legislation is taking place. The revised hygiene legislation provides also, among other things, a legal basis to set specific temperature control requirements for foodstuffs, when appropriate. QuestionThe European ...

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Section: Emetic Foodborne Intoxicationmentioning

confidence: 99%