Comparative studies of selective media for recovery of Staphylococcus aureus from natural waters

Abstract

Several selective media currently used for the enumeration of Staphylococcus aureus from different sources were evaluated in order to establish their quantitative recovery, specificity and degree of selectivity, using different types of water samples. The highest selectivity and reliability in the enumeration of Staph. aureus from the samples was obtained on Borrego-Florido-Romero-0 (BFR-0) and KRANEP agars. The method that produced the highest recovery of Staph. aureus was BFR-0 agar with membrane filter and incubation at 36 degrees C for 48-72 h.

Estudio De Microorganismos Patógenos en La Fuente Termal De O Tinteiro en Ourense Study of Microbial Pathogens in the Hot Spring of Tinteiro at Ourense Estudo De Microorganismos Patóxenos Na Fonte Termal Do Tinteiro en Ourense

“…Ante la aparición de colonias sospechosas de color blanco o crema, fueron sembradas en medio Vogel-Johnson a 37ºC durante 24 horas tomándose como colonias típicas aquéllas de color negro rodeadas de halo amarillo. Posteriormente se procedió a su aislamiento e identificación utilizando las pruebas bioquímicas de Gram, Movilidad, O/F, Oxidasa, Catalasa, DNAasa Nitratos, Arginina, Xilosa, Arabinosa y Sacarosa (Alico y Dragonjac, 1986;APHA et al, 1985;Borrego, 1992;Borrego et al, 1988;Kloss y Schleifer, 1986).…”