Comparative studies of the effects of copper sulfate and zinc sulfate on serum albumins

Abstract

The work is devoted to the study of the interaction of heavy metals with bovine serum albumin (BSA) and human serum albumin (HSA), by quenching of the intrinsic fluorescence of proteins and fluorescent probe pyrene by heavy metal ions. Sulfates of copper and zinc (CuSO 4 , ZnSO 4 ) were taken as the metal salts. The value of the Stern-Volmer constants of quenching of intrinsic fluorescence of proteins and fluorescence probe pyrene reduced from Cu (II) to the Zn (II). It was experimentally found that the copper ions have a greater ability to fluorescence quenching, which is probably associated with the greater availability of protein chromophore groups to copper ions and with adsorbed fluorescent probe pyrene in the protein globule.

Immobilization of transaminase from Bacillus licheniformis on copper phosphate nanoflowers and its potential application in the kinetic resolution of RS-α-methyl benzyl amine

“…TA@Cu 3 (PO 4 ) 2 NF did not show any notable λ em shift, which showed a preservation of the tertiary enzyme structure inside the immobilized matrix. The quenching effect was observed due to the Cu (II) metal ion (Plotnikova et al 2016 ).…”