Comparative studies on analytical, antioxidant, and antimicrobial activities of a series of vegetal extracts prepared from eight plant species growing in Romania

Abstract

SummaryGiven the large applicability in both pharmaceutical and cosmetic field, this work was aimed at analytical (high-performance thin-layer chromatography [HPTLC] method), antioxidant (2,2-diphenyl-1-picrylhydrazyl [DPPH] method), and antimicrobial (diffusion method on Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, and Candida albicans ATCC 10231) studies on a series of whole vegetal extracts and corresponding aqueous, ethyl acetate and chloroform fractions (selective extracts) prepared from eight plant species growing in Romania. Briefly, it was revealed moderate to certain activity against S. aureus ATCC 6538 and E. coli ATCC 8739 in the case of greater burdock leaves (Arctium lappa), beech leaves (Fagus sylvatica) and great willowherb aerial part (Epilobium hirsutum) whole extracts. Purple loosestrife aerial part (Lythrum salicaria) and sea-buckthorn leaves (Hippophae rhamnoides) whole extracts showed only weak activity against these bacteria, while tarragon aerial part (Artemisia dracunculoides), chokeberries leaves

Polyphenols Content, Antioxidant and Antimicrobial Activity of Ethanol Extracts from the Aerial Part of Rock Rose (Helianthemum nummularium) Species

“…1, gallic acid has been confirmed occurrence in both Hnet70 sample (see T3 track from chromatogram B1, the blue-indigo fluorescent spot at Rf ≈ 0.38) and related hydrolysed sample HnH (see T3H track from chromatogram B2, the blue-indigo fluorescent spot at Rf ≈ 0.42). Furthermore, the hydrolysed sample HnH (T3H track, chromatogram B2) have revealed important quantities of kaempferol aglycone (green fluorescent spot at Rf ≈ 0.88) [10], supporting s2, s3 and s4 spots attribution to kaempferol derivates (sub) class as well.…”

“…Polyphenols analysis was performed by HPTLC method according to Wagner and Bladt [8] and Reich and Schibli [9], and the standard setting for polyphenols (1, ethyl acetate: formic acid: glacial acetic acid: water/100:11:11:26) and saponins (2, chloroform: glacial acetic acid: methanol: water /64:32:12:8) assay, as described in previous work [10].…”

“…Total phenols were evaluated by using Folin-Ciocalteau reagent according to the standard method of "Romanian Pharmacopoeias (FRX)" [11], which was also described in previous work [10]. The results were expressed as gallic acid equivalents (GAE).…”

“…Microbiological tests were performed using cylinder method in plates according to "Romanian Pharmacopoeias" [11] standard method, which was also detailed in the authorts' previous work [10]. Four standard microbial strains, three gram-negative (Escherichia coli ATCC 8739, Salmonella typhimurium ATCC 14028 and Salmonella enterritidis ATCC 13076) and one gram-positive (Staphylococcus aureus ATCC 6538) bacteria were used.…”

“…The saponins system (series 2) was demonstrated as very useful for polyphenols aglycones separation, too [10]. In series 2 of rock rose ethanolic extracts (Hnet70), as shown in chromatograms B in Fig.…”