Comparative Studies on Antioxidant and Anti-Inflammatory Activities of Ethanolic Extracts of True Mangrove and Mangrove Associate Located in Bhatye Beach Areas of Maharashtra, India

Abstract

The present study showed that among the phytochemicals both the mangroves revealed presence of flavonoids, tannins, triterpenes, alkaloids and saponins. Antioxidant and Anti-inflammatory activity of leaves, stems, pods and fruits of ethanolic extract of Derris trifoliata and Sonneratia alba mangrove species were studied. Total flavonoid content (TFC) was found to be maximum in S.alba fruits of ethanolic extract (792.6 mg quercetin equivalent QE/g) as well as the total phenolic content (TPC) was found to be highest (630.39 mg gallic acid equivalent GAE/g). Ethanolic extract at a concentration range (20-100 μg/mL) showed antioxidant and anti-inflammatory assay where free radical and hydrogen peroxide scavenging potentials of S. alba fruits IC50 value (62.62 and 66.73 μg/mL) was greater as compared to other extracts respectively. The reducing power of all the extract of both the plant increased dose-dependently in which S.alba fruits showed maximum reducing power. Anti-inflammatory activity protects membrane stabilization, protein denaturation and also protease inhibition. In membrane stabilization, S.alba fruits revealed higher activity which represented 64.67 ± 0.05% protection and lower in D.trifoliata pods 46.97 ± 0.06% protection. All the ethanolic extracts displayed inhibition where S.alba fruits exhibited 80.07 ± 0.02% inhibition of proteinase activity. A concentration dependent inhibition of protein (albumin) denaturation where maximum amount of inhibition was found in S.alba fruits (84.67±0.04% inhibition). Present results confirmed that among the selected parts of mangroves ethanolic fruits extracts of S.alba showed more potent antioxidant and anti-inflammatory activities.

Exploring the Anti-inflammatory Potential of Mangrove Flora: A Comprehensive Review

“…Kumari et al70 dealt with meth-anolic leaf extract of R. mucronata of southeast coast of India, which notably demonstrated anti-inflammatory action at the concentration of 400 mg equivalent to the conventional medicine diclofenac sodium. There was a comparable investigation done by Ray et al [71], who gathered mangrove samples of Sundarban mangrove environment and observed that R. mucronata leaves displayed an in-vitro anti-inflammatory activity in RAW264.7 cell line by MTT test. The impact of the ethanolic extract of R. mucronata leaves (25,50,100,200 and 400µg/ml) on the viability of unstimulated RAW264.7 cells was examined using the MTT assay.…”

“…Mangrove plants have been employed for their therapeutic characteristics by the coastal inhabitants of India for the treatment of inflammation, painful arthritis, antioxidant, diabetes, hepatoprotective activities, asthma, tu-mor, rheumatism, anti-nociceptive and antipyretic activity [63][64][65][66]71]. However, in the Indian environment, on-ly restricted investigations have been undertaken for the separation and purification of bioactive chemicals from mangroves plants.…”

In Vitro Antidiabetic Activity and Phytochemical Analysis of Lumnitzera Racemosa Leaves

“…The medicinal properties of the plant depend upon its phytochemical composition. The mangroves have shown diverse classes of secondary metabolites therefore possess several medicinal properties like antimicrobial 25 , antiviral activities 26 , antioxidant 27 , and anti-inflamatory 28 . The antidiabetic properties of mangrove plants are related to alkaloid, flavonoid, saponin and terpenoids 29 .…”