Comparative studies on bull and stallion seminal DNase activity and interaction with semen extender and spermatozoa

Alghamdi, Abdorrahman S.; Funnell, Bethany J.; Bird, Scott; Lamb, G. C.; Rendahl, Aaron; Taube, Patrick C.; Foster, Douglas N.

doi:10.1016/j.anireprosci.2010.06.003

Cited by 21 publications

(26 citation statements)

References 44 publications

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“…This is especially important because it suggests a testicular origin of sperm DNA alteration. It has been suggested that peptides with a DNASE domain in seminal plasma from bovines and equines are fertility markers [47]. With our study, we demonstrate that alterations seminal levels of DNASE1 and IGFBP7 can identify testicular alterations in varicocele; however, it remains to be verified if this is true only for varicocele, or if these proteins identify alterations in testicular function in other conditions related to male infertility as well.…”

Section: Discussionmentioning

confidence: 49%

Alterations in the proliferative/apoptotic equilibrium in semen of adolescents with varicocele

Belardin

Giudice

Camargo

et al. 2016

J Assist Reprod Genet

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Purpose To verify if the presence of varicocele (grades II and III) with and without seminal alterations, using the 5 th centile cutoff values in table A1.1 of the World Health Organization (WHO, 2010) manual, alters the seminal plasma levels of proteins DNASE1 (deoxyribonuclease-1) and IGFBP7 (Insulin-like growth factor-binding protein 7), which are related to apoptosis regulation and cell proliferation, respectively, demonstrating that these proteins are important for correct spermatogenesis. Methods This cross sectional study was performed at the Sao Paulo Federal University Paulo between May 2014 and April 2016. A total of 61 male adolescents were included in this study, of which 20 controls without varicocele (C), 22 with varicocele and normal semen analysis (VNS) and 19 with varicocele and altered semen analysis (VAS). Seminal plasma from each patient was used for Western blotting analysis of individual protein levels. Values of each protein were normalized to a testicular housekeeping protein (PARK7-protein deglycase DJ-1). Results Levels of IGFBP7 protein are increased in varicocele. Levels of DNASE1 are progressively decreased in varicocele (lower in varicocele and normal semen analysis, lowest in varicocele and altered semen analysis) when compared to adolescents without varicocele. DNASE1 levels are positively correlated with sperm concentration and morphology (correlation values of 0.400 and 0.404, respectively; p values of 0.001 and 0.001, respectively). Conclusion In conclusion, in adolescents, seminal plasma levels of IGFBP7, responsible for proliferative activity, are increased in varicocele grades II and III, and DNASE1, responsible for apoptosis regulation, are lower in varicocele, lowest in varicocele and low semen quality. These proteins demonstrate molecular alterations brought upon by varicocele. Moreover, DNASE1 is capable of discriminating a varicocele that causes alterations to semen quality from one that does not. We propose that the initial response of varicocele is to increase proliferative activity which, if followed by regulation of apoptosis, may lead to the ejaculation of a population of sperm that are in accordance with WHO cutoff values but, in the presence of dysregulated apoptosis, leads to lower sperm concentration and morphology.

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Section: Discussionmentioning

confidence: 49%

Alterations in the proliferative/apoptotic equilibrium in semen of adolescents with varicocele

Belardin

Giudice

Camargo

et al. 2016

J Assist Reprod Genet

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“…DNase I is an extracellular protein involved in regulation of apoptosis (Kishi et al ., ). DNases in seminal plasma have been shown to free spermatozoa from neutrophil binding after insemination in animals (Alghamdi & Foster, ) and activity of DNases (or DNase‐like peptides) are considered markers of fertility (Alghamdi et al ., ). In our study, its presence as a marker of controls may be indicative of maintenance of fertility potential in humans.…”

Section: Discussionmentioning

confidence: 97%

Determination of testicular function in adolescents with varicocoele – a proteomics approach

et al. 2016

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SUMMARYThe goal of this study was to determine seminal plasma biomarkers of testicular function in adolescents with varicocoele and to verify enriched gene ontology terms associated to these differential proteomes. An observational study was carried out in an academic research environment. A total of 77 adolescent patients were recruited from a local public school, of which 23 were without varicocoele and with normal semen analysis (control group), 37 were with varicocoele and normal semen (VNS) parameters, and 17 were with varicocoele and altered semen (VAS) parameters. Two semen collections were provided with a 1-week interval, after 2-5 days of ejaculatory abstinence. Seminal plasma proteins were identified and quantified utilizing a label-free shotgun proteomics approach, generating (i) proteins differentially expressed in each group (control, VNS, and VAS) and putative biomarkers using multivariate statistics followed by discriminant analysis. Confirmatory analysis was performed for two proteins by western blotting. Enriched biological processes and molecular functions were determined using gene ontology analysis. In total, 541 proteins were identified and quantified: 108 exclusive or overexpressed in controls, 26 in the VNS group, and 13 in the VAS group. The suggested biomarkers are Cab45/SDF4 (Q9BRK5), protein lefty-1 (O75610), DNase I (P24855), PAP2-alpha (O14494), IBP-7 (Q16270), HDC (P01860), and CRISP-3 (P54108). Western blotting results showed that Cab45 was significantly underexpressed in both varicocoele groups, and CRISP-3 was significantly overexpressed in seminal plasma of adolescents with VAS. In conclusion, specific biomarkers of spermatogenesis and homeostasis are observed in adolescents without varicocoele, and the presence of a palpable varicocoele progressively shifts these adolescents toward initially an immune response, and finally toward a chronic inflammatory profile. This shift is accompanied by decreased semen quality.

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“…; Alghamdi et al. ). Despite this, the potential effects of seminal plasma in the uterus are pertinent, given the common practice of intrauterine AI to yield a higher reproductive efficiency.…”

Section: Evidence For Seminal Fluid Signalling In Various Mammalian Smentioning

confidence: 98%

Seminal Fluid and Immune Adaptation for Pregnancy – Comparative Biology in Mammalian Species

Schjenken

Robertson

2014

Reprod Domestic Animals

109

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Seminal fluid delivered to the female reproductive tract at coitus not only promotes the survival and fertilizing capacity of spermatozoa, but also contains potent signalling agents that influence female reproductive physiology to improve the chances of conception and reproductive success. Male to female seminal fluid signalling occurs in rodents, domestic and livestock animals, and all other mammals examined to date. Seminal plasma is instrumental in eliciting the female response, by provision of cytokines and prostaglandins synthesized in the male accessory glands. These agents bind to receptors on target cells in the cervix and uterus, activating changes in gene expression leading to functional adaptations in the female tissues. Sperm also interact with female tract cells, although the molecular basis of this interaction is not yet defined. The consequences are increased sperm survival and fertilization rates, conditioning of the female immune response to tolerate semen and the conceptus, and molecular and cellular changes in the endometrium that facilitate embryo development and implantation. Studies in porcine, equine, bovine, ovine and canine species all show evidence of male-female signalling function for seminal fluid. There are variations between species that relate to their different reproductive strategies and behaviours, particularly the site of seminal fluid deposition and female reproductive tract anatomy. Although the details of the molecular mechanisms require more study, the available data are consistent with both the sperm and plasma fractions of seminal fluid acting in a synergistic fashion to activate inflammation-like responses and downstream female tract changes in each of these species. Insight into the biological function and molecular basis of seminal fluid signalling in the female will inform new interventions and management practices to support optimal reproductive outcomes in domestic, livestock and endangered animal species.

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Comparative studies on bull and stallion seminal DNase activity and interaction with semen extender and spermatozoa

Cited by 21 publications

References 44 publications

Alterations in the proliferative/apoptotic equilibrium in semen of adolescents with varicocele

Alterations in the proliferative/apoptotic equilibrium in semen of adolescents with varicocele

Determination of testicular function in adolescents with varicocoele – a proteomics approach

Seminal Fluid and Immune Adaptation for Pregnancy – Comparative Biology in Mammalian Species

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