ABSTRACT-Mosquitoes are important groups of arthropods that inhabit freshwater habitats. The distribution pattern of adult mosquitoes is related to habitat preference of the immature stages. These habitats may be natural or man-made and temporary or permanent. Mosquitoes are carriers of number of diseases; mostly in the tropics, causing illness and death on a large scale. The survey was carried out during June 2015 to May 2016 from different ten villages of Kopargaon teshil (M.S.). Mosquito larvae were collected at different habitats, these are temporary and permanent, larvae collections were carried out regular month wise. A total 3627 mosquito larvae were collected of which were density of Culicinae were 90.21% and Anophelinae were 9.79%. During the study period seven mosquito species were identified, which are Anopheles stephensi, Culex vishnui, Culex pseudovishnui, Culex quinquefasciatus, Aedes aegypti, Aedes albopictus and Armigeres subalbatus. Their densities are Anopheles stephensi 9.79%, Culex vishnui 13.51%, Culex pseudovishnui 10.34%, Culex quinquefasciatus 7.2%, Aedes aegypti 26.16%, Aedes albopictus 15.08% and Armigeres subalbatus 17.92% respectively. The mosquito larval fauna providing primary checklist of mosquito vector diversity from study area.