Comparative studies on nebulizers for antigen inhalation in experimental asthma

Nabe, Takeshi; Shinoda, Naoki; Yamashita, Kazunori; Yamada, Masahiro; Yamamura, Hideki; Kohno, Shigekatsu

doi:10.2332/allergolint.46.261

Cited by 44 publications

(25 citation statements)

References 16 publications

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“…Resultantly, when guinea pigs were forced to inhale the mists, approx. 80% of the trapped mists in the airways was found in the lung, but no more than 50% and 20%, respectively, of the other two-generated mists arrived in the lung (24). These observations suggest not only that the LAR observed in the OA + Al(OH)3/OA group was largely reflected by responses at the lung, but also that the present LAR was elicited with the result that the inhaled Al(OH)3 together with antigen had activated some immune systems.…”

Section: Discussionmentioning

confidence: 99%

Repeated Antigen Inhalation-Induced Reproducible Early and Late Asthma in Guinea Pigs.

et al. 1997

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ABSTRACT-To develop a model of chronic experimental asthma in guinea pigs, the animal was forced to inhale the mist of a low dose of ovalbumin (OA) adsorbed on fine Al(OH)3 for sensitization once every 4 weeks. The animal was challenged by inhalation with the mist of OA on day 14 after the respective sensitizations. Either the first or the second antigen challenge markedly induced an early asthmatic response (EAR), whereas there was hardly any late asthmatic response (LAR). At the 3rd challenge, LAR also emerged with some severity. These dual responses were consistently observed until the 10th challenge. On the other hand, repeated inhalation/challenge, once every 2 weeks, with OA alone at the same dose tended to lead to the desensitization of the EAR. In addition, LAR was hardly observed throughout the experiments. In both groups, Ti and IgE levels in the serum were elevated by the repetitive antigen inhalations, yet no obvious relationship between these antibody levels and the intensity of either EAR or LAR was recognized. The present results indicate that the asthmatic model with reproducible EAR and LAR developed in this study appears to be very beneficial for the investigation of bronchial asthma and for the assessment of anti-asthma drugs.

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Section: Discussionmentioning

confidence: 99%

Repeated Antigen Inhalation-Induced Reproducible Early and Late Asthma in Guinea Pigs.

et al. 1997

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“…Cedar pollen extracts used for the sensitization were prepared as previously described (13). Al(OH) 3 gels were prepared from 0.25 N NaOH and 0.25 N Al 2 (SO 4 ) 3 , as previously described (16).…”

Section: Methodsmentioning

confidence: 99%

Acquired Nasal Hyperresponsiveness Aggravates Antigen-Induced Rhinitis in the Guinea Pig

et al. 2003

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Abstract. Whether a state of nasal hyperresponsiveness influences antigen-induced biphasic nasal blockage and sneezing were examined using a guinea pig model of allergic rhinitis. Sensitized animals were challenged with an antigen, Japanese cedar pollen, once every week. Before the 13th challenge, the animals were randomly divided into 2 groups, and then the 13th challenge was performed (Groups A-0 and B-0). The 14th challenge was done on day 2 (Group A-2) and on day 7 (Group B-7) after the 13th challenge, on which nasal hyperresponsiveness was present and absent, respectively. Biphasic nasal blockage and sneezing after the challenge in Group A-2 were more severe than those in Group A-0, while those of Group B-7 were almost the same as those of Group B-0. An anti-histaminic, mepyramine, inhibited sneezing but not the biphasic nasal blockage in Group B-7. A cysteinyl leukotriene (CysLT) antagonist, pranlukast, suppressed the late nasal blockage but not the early blockage and sneezing in Group B-7. In contrast, in Group A-2, mepyramine significantly attenuated not only sneezing but also the early nasal blockage. Pranlukast significantly inhibited both nasal blockage and sneezing in Group A-2. In conclusion, nasal hyperresponsiveness aggravated the antigen-induced nasal responses, to which histamine and CysLTs considerably contributed.

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“…Al(OH) 3 gels were prepared with 0.25 N NaOH and 0.25 N Al 2 (SO 4 ) 3 , as previously described (17).…”

Section: Antigen and Adjuvantmentioning

confidence: 99%

Effect of Oral Immunotherapy on Nasal Blockage in Experimental Allergic Rhinitis

et al. 2005

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Abstract. We previously reported that when Japanese cedar pollen was prophylactically p.o. administered before a sensitization stage in a guinea-pig model of allergic rhinitis, pollen-induced nasal blockage was suppressed. In this study, we evaluated whether the oral immunotherapy is also effective when the pollen extract was administered starting from the day when the nasal blockage was clearly induced and whether the effectiveness continued after cessation of the immunotherapy. Sensitized animals were repeatedly challenged by pollen inhalation once every week. After the 7th challenge, the extract was orally administered twice a week until the 30th challenge. At the 11th challenge, the oral immunotherapy showed inhibition of the biphasic nasal blockage. The effectiveness was consistently observed during the immunotherapy until the 30th challenge. Furthermore, the increased nasal responsiveness to intranasal application of leukotriene D 4 was markedly suppressed by the immunotherapy. Interestingly, even after cessation of the therapy, inhibition of the nasal blockage was sustained for more than 2 months. Nevertheless, neither sneezing nor antigen-specific IgE antibody production was substantially influenced by the immunotherapy. In conclusion, Oral immunotherapy may be clinically useful for allergic nasal blockage. Mechanisms underlying the effectiveness may be associated with the hyporesponsiveness of the nasal mucosa to released mediators.

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Comparative studies on nebulizers for antigen inhalation in experimental asthma

Cited by 44 publications

References 16 publications

Repeated Antigen Inhalation-Induced Reproducible Early and Late Asthma in Guinea Pigs.

Repeated Antigen Inhalation-Induced Reproducible Early and Late Asthma in Guinea Pigs.

Acquired Nasal Hyperresponsiveness Aggravates Antigen-Induced Rhinitis in the Guinea Pig

Effect of Oral Immunotherapy on Nasal Blockage in Experimental Allergic Rhinitis

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