Comparative studies on [Pasteurella] testudinis and [P.] testudinis-like bacteria and proposal of Chelonobacter oris gen. nov., sp. nov. as a new member of the family Pasteurellaceae

Gregersen, R. H.; Neubauer, C.; Bojesen, Anders Miki; Heß, Michael; Bisgaard, Magne

doi:10.1099/ijs.0.007203-0

Cited by 35 publications

(10 citation statements)

References 21 publications

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“…All Chelonobacter oris strains were isolated from the oral cavity with the exception of one that was isolated from an abscess, one from conjunctiva and two from the nares (Gregersen et al, 2009). One strain isolated from a skin infection in a yellow-bellied slider (Trachemys scripta scripta) showed a genus-like relationship with Pasteurella testudinis (Gregersen et al, 2009). This strain is, to our knowledge, the only described Pasteurellaceae strain previously isolated from a freshwater turtle while there is no description of Pasteurellaceae isolates from sea turtles in the literature.…”

Section: Elnt2xmentioning

confidence: 99%

“…Pasteurella testudinis has exclusively been isolated from Hermann's tortoises (Testudo hermanni) (Snipes & Biberstein, 1982) while Chelonobacter oris was isolated from diseased Hermann's and desert tortoises (Gopherus agassizi) (Gregersen et al, 2009). Pasteurella testudinis was isolated from the oral cavity of tortoises showing clinical signs of respiratory disease and from the cloacae and oral cavity of healthy tortoises (Snipes & Biberstein, 1982).…”

Section: Elnt2xmentioning

confidence: 99%

“…Pasteurella testudinis was isolated from the oral cavity of tortoises showing clinical signs of respiratory disease and from the cloacae and oral cavity of healthy tortoises (Snipes & Biberstein, 1982). All Chelonobacter oris strains were isolated from the oral cavity with the exception of one that was isolated from an abscess, one from conjunctiva and two from the nares (Gregersen et al, 2009). One strain isolated from a skin infection in a yellow-bellied slider (Trachemys scripta scripta) showed a genus-like relationship with Pasteurella testudinis (Gregersen et al, 2009).…”

Section: Elnt2xmentioning

confidence: 99%

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Testudinibacter aquarius gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of freshwater turtles

Hansen

Pennanen

Bojesen

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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Section: Elnt2xmentioning

confidence: 99%

Section: Elnt2xmentioning

confidence: 99%

Section: Elnt2xmentioning

confidence: 99%

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Testudinibacter aquarius gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of freshwater turtles

Hansen

Pennanen

Bojesen

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…Using the pyrosequencing results, we sought to determine whether Pasteurella testudinis, a possible pathogen in Gopherus tortoises [21], was present in each sample. A study by Gregersen et al [55] separated the genus Chelonobacter from other Pasteurellaceae, with Chelonobacter and P. testudinis forming a monophyletic group sister to other Pasteurellaceae including other Pasteurella. The reference database used in the present study, Greengenes [47,49] identified OTUs in our analyses as belonging in the Chelonobacter genus.…”

Section: Bacterial Communities Among Host Speciesmentioning

confidence: 99%

Host species, pathogens and disease associated with divergent nasal microbial communities in tortoises

2018

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Diverse bacterial communities are found on every surface of macro-organisms, and they play important roles in maintaining normal physiological functions in their hosts. While the study of microbiomes has expanded with the influx of data enabled by recent technological advances, microbiome research in reptiles lags behind other organisms. We sequenced the nasal microbiomes in a sample of four North American tortoise species, and we found differing community compositions among tortoise species and sampling sites, with higher richness and diversity in Texas and Sonoran desert tortoises. Using these data, we investigated the prevalence and operational taxonomic unit (OTU) diversity of the potential pathogen Pasteurella testudinis and found it to be common, abundant and highly diverse. However, the presence of this bacterium was not associated with differences in bacterial community composition within host species. We also found that the presence of nasal discharge from tortoises at the time of sampling was associated with a decline in diversity and a change in microbiome composition, which we posit is due to the harsh epithelial environment associated with immune responses. Repeated sampling across seasons, and at different points of pathogen colonization, should contribute to our understanding of the causes and consequences of different bacterial communities in these long-lived hosts.

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“…The family Pasteurellaceae currently contains 14 recognized genera and over 60 species with validly published names (Christensen & Bisgaard, 2008;Gregersen et al, 2009). These bacteria are commonly isolated from a broad range of vertebrate species, where they belong to the normal flora of mucosal membranes, some also acting as opportunistic pathogens (Kuhnert & Christensen, 2008).…”

mentioning

confidence: 99%

Basfia succiniciproducens gen. nov., sp. nov., a new member of the family Pasteurellaceae isolated from bovine rumen

Kuhnert

Scholten

Haefner

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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Gram-negative, coccoid, non-motile bacteria that are catalase-, urease- and indole-negative, facultatively anaerobic and oxidase-positive were isolated from the bovine rumen using an improved selective medium for members of the Pasteurellaceae. All strains produced significant amounts of succinic acid under anaerobic conditions with glucose as substrate. Phenotypic characterization and multilocus sequence analysis (MLSA) using 16S rRNA, rpoB, infB and recN genes were performed on seven independent isolates. All four genes showed high sequence similarity to their counterparts in the genome sequence of the patent strain MBEL55E, but less than 95 % 16S rRNA gene sequence similarity to any other species of the Pasteurellaceae. Genetically these strains form a very homogeneous group in individual as well as combined phylogenetic trees, clearly separated from other genera of the family from which they can also be separated based on phenotypic markers. Genome relatedness as deduced from the recN gene showed high interspecies similarities, but again low similarity to any of the established genera of the family. No toxicity towards bovine, human or fish cells was observed and no RTX toxin genes were detected in members of the new taxon. Based on phylogenetic clustering in the MLSA analysis, the low genetic similarity to other genera and the phenotypic distinction, we suggest to classify these bovine rumen isolates as Basfia succiniciproducens gen. nov., sp. nov. The type strain is JF4016T (=DSM 22022T =CCUG 57335T).

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Comparative studies on [Pasteurella] testudinis and [P.] testudinis-like bacteria and proposal of Chelonobacter oris gen. nov., sp. nov. as a new member of the family Pasteurellaceae

Cited by 35 publications

References 21 publications

Testudinibacter aquarius gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of freshwater turtles

Testudinibacter aquarius gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of freshwater turtles

Host species, pathogens and disease associated with divergent nasal microbial communities in tortoises

Basfia succiniciproducens gen. nov., sp. nov., a new member of the family Pasteurellaceae isolated from bovine rumen

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